The invention discloses a preparation method of membrane-filtrated concentrated fermented milk. The preparation method of the membrane-filtrated concentrated fermented milk specifically comprises thefollowing steps: S1, performing quality inspection on raw-material milk; S2, treating the inspected raw-material milk by using membrane concentration equipment; S3, blending materials, and carrying out
standardization; S4, carrying out degassing, namely performing degassing on the pre-
heated milk material by using a degassing tank; S5, carrying out homogenization, namely performing homogenizationon the degassed milk material under a pressure of 170-180 Bar; S6, carrying out sterilization, namely performing sterilization on the homogenized milk material at 93-97 DEG C for 300 seconds; S7, carrying out cooling, namely cooling the sterilized material to be 40-43 DEG C by using a
heat exchanger, and sending the cooled material into a
fermentation tank; S8, carrying out
inoculation, namely adding confirmed bacterial strains, under aseptic operation, into an on-line adding
system, wherein the adding starts when the sterilization on the material is 1 / 3 done; S9, carrying out
thermal insulation, namely performing thermally insulated
fermentation on the inoculated material, at 40-42 DEG C, for 4-6 hours; S10, carrying out cooling; and S11, carrying out filling.