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Capillary micro-channel assistant wire base microfluidic bipolar electrode electrochemical luminescence device and application

A bipolar electrode and light-emitting device technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve problems that have not been reported before, and achieve fast sample loading, good flexibility, and simple processing. Effect

Active Publication Date: 2017-01-25
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the sensing method and device combining bipolar electrode electrochemiluminescence and line-based microfluidics have not been reported.

Method used

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  • Capillary micro-channel assistant wire base microfluidic bipolar electrode electrochemical luminescence device and application
  • Capillary micro-channel assistant wire base microfluidic bipolar electrode electrochemical luminescence device and application
  • Capillary micro-channel assistant wire base microfluidic bipolar electrode electrochemical luminescence device and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] A preparation method of capillary microchannel-assisted line-based microfluidic bipolar electrode electrochemiluminescence device, comprising the following steps:

[0069] (1) Use Adobe Illustrate CS5 drawing software to design the size of the wire base electrode and the bare wire area of ​​the patterned wire electrode, and then process it into a 300-mesh polyester stencil by Guangzhou Lianchang Printing Equipment Store;

[0070] (2) Take a hydrophilic white cotton thread ball (the thread diameter is about 0.5mm), cut out 16 lines with a length of 100mm with scissors; Flatly fixed on the smooth glass processing table, and the screen is attached to the line array;

[0071] (3) Conductive carbon paste (figure 1 shown).

[0072] From figure 1 It can be seen that the method of the present invention can be well processed into 16 patterned wire electrodes, each of which has 2 bare wire regions 3 (about 12 mm in length), 2 driving electrodes 1 (about 7 mm in length), and 1 b...

Embodiment 2

[0080] The application of the capillary microchannel-assisted line-based microfluidic device prepared in Example 1 in the electrochemiluminescence detection of TPA at the amphoteric electrode includes the following steps:

[0081] (a) Use alligator clips to connect the positive drive electrode and the negative drive electrode of the capillary microchannel auxiliary line-based microfluidic device to the positive and negative poles of a DC power supply (model PS-305D), respectively, and the power supply is turned off;

[0082] (b) Use a pipette gun to drop a certain volume of test solution near the port of the capillary microchannel, and wait for a few seconds to make the line between the driving electrodes and the capillary full of test solution. The test solution is PBS (0.1M) with a pH value of 6.9 to prepare a 10mM TPA solution and a 2.0mM Ru(bpy) 3 2+ The solutions are obtained by mixing in a volume ratio of 1:1.

[0083](c) Then put the bracket and the light-emitting dev...

Embodiment 3

[0090] 2 important factors (capillary microchannel length L CMC , driving voltage E tot ) for optimization

[0091] a) Preferred capillary microchannel length L CMC

[0092] (1) other conditions are the same as embodiment 2, i.e. T screen =17,L BPE = ~ 7mm; E tot =22V; V assay = 13-17 μL.

[0093] (2) Set up several experimental groups: capillary microchannel length L CMC Set to several different values ​​(12mm, 16mm, 20mm, 24mm, 28mm).

[0094] (3) detection and analysis process are with embodiment 2, and experimental result is as follows Figure 6 shown.

[0095] It can be seen from the experimental results that the luminous intensity increases with the L CMC It is gradually enhanced when changing from 12mm to 24mm. The possible reason is that in the same E tot Under the condition, the longer capillary microchannel can load more test liquid, resulting in a larger internal electric field intensity, which leads to a larger potential difference between the two ends...

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Abstract

The invention discloses a capillary micro-channel assistant wire base microfluidic bipolar electrode electrochemical luminescence device, and a preparation method and the application thereof. The luminescence device consists of a capillary and a patterning line electrode, wherein the patterning line electrode consists of not less than three wire base electrodes and bare wire areas; the wire base electrodes are manufactured by conductive carbon paste; the wire base electrodes on two sides are driving electrodes; the wire base electrode between the driving electrodes is a bipolar electrode; an area between the every two wire base electrodes is the bare wire area; the capillary coats the patterning line electrode, needs to coat all bipolar electrodes, and simultaneously can not be in contact with the driving electrode. According to the luminescence device, the capillary micro-channel is applied to the line of the patterning carbon electrode for the first time to manufacture the capillary micro-channel assistant wire base microfluidic bipolar electrode electrochemical luminescence device. The integration device has the remarkable advantages that the loading speed of a sample is high and the samples can be evenly distributed on the patterning wire electrode.

Description

technical field [0001] The invention belongs to the sensing field of microfluidic devices, and in particular relates to a capillary microchannel auxiliary line-based microfluidic bipolar electrode electrochemiluminescent device, a preparation method and an application thereof. Background technique [0002] In recent years, microfluidic devices have been widely used in the fields of biochemistry and medical testing. It has the advantages of significantly reducing reagent consumption, low price, and high analysis efficiency. It has become one of the frontier research hotspots in the field of modern analysis and has a huge market demand. and good development prospects. [0003] Microfluidic analysis methods and devices based on various fiber materials (such as paper, cloth, cotton thread, etc.) have attracted much attention. The gaps and wettability of the internal fibers of these materials can cause the liquid to flow capillary in the fiber material only by capillary force, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 章春笋刘瑞
Owner SOUTH CHINA NORMAL UNIVERSITY
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