51results about How to "Strong fluorescence response" patented technology

The invention relates to a use method for a creatinine content detection kit, which includes the following steps: adding creatinine of different concentrations to a buffer solution with the pH value of 7.3 and containing 100 mM of MHEPES, 150 mM of sodium chloride, 5 mM of potassium chloride and 1.3 mM of calcium chloride; obtaining and metering 150 muL sample solution through a capillary tube, inserting the tube in the kit and measuring through a capillary tube. The method provided by the invention has high selectivity; creatininase as proteinase has high specificity to a reactant, only catalyzes the hydrolysis reaction of creatinine, and has no catalytic activity to other oligomers; as three high selectivity enzymes are related during the detection, the selectivity in the method is increased exponentially, the final response of the kit is fluorescent response, and compared with other methods for detecting color change, the method adopting the fluorescent response has the advantage that the inherent characteristic of fluorescent response endows the method with higher sensitivity and anti-interference capability.

The invention discloses a kit for detecting bleomycin based on a double-ring hairpin probe and an enzyme-mediated cascade amplification strategy and a detection method of the kit. The double-ring hairpin probe can effectively silence a trigger sequence and inhibit non-specific hybridization, and therefore a background signal of the detection method is reduced; an enzyme-mediated cascade amplification reaction can achieve multiple amplification of a detection signal, therefore, the sensitivity of the detection method is improved, high-sensitivity detection of bleomycin is achieved, the linear range is 40 pM to 10 nM, and the detection limit is 35 pM. The sensitivity is improved by about two orders of magnitudes compared with a previously established label-free fluorescence detection method, and the requirements of clinical sample detection can be met.

The invention relates to a fluorescent probe molecule for detecting explosive RDX and its preparation method and application. The method uses pyrrole and p-tolualdehyde as reactants to first obtain chlorofluoroboron dipyrrole derivatives, and then combine them with Hydrazine hydrate was reacted at room temperature for 12 hours, and then separated by column chromatography to obtain a fluorescent probe molecule substituted with a hydrazine group. Furthermore, RDX can be detected indirectly through the response of the fluorescent probe to the photolysis product of explosive RDX. The method has mild reaction conditions and simple test conditions, and the probe molecules have high sensitivity and good selectivity to explosives RDX, which is a feasible attempt in the application of fluorescent sensors to explosives detection.

The invention discloses an organic ligand for synthesizing metal organic frames. The chemical nomenclature of the organic ligand is 2-(3, 5-bis (4-pyridyl) phenyl) benzoglioxaline. The chemical formula of the organic ligand is represented in the description. The invention also discloses a synthetic method of the organic ligand and a Cu (I) ion-based bright yellow metal organic frame (MOF-1) and a Cu (I) ion-based dark red metal organic frame (MOF-2), and the chemical formulas are {[Cu(L)I].0.5CH3CN.0.5CH3OH.0.75H2O}n, (n=1,2,3...'8') and {[Cu(L)I].2H2O}n(n=1,2,3...'8'). The invention also provides a synthetic method for dark red Cu (I) ion metal organic frame (MOF-2) and a synthetic method for organic matters of the Cu (I) ion metal organic frame. The invention also provides application of the organic ligand and metal organic frames serving as alcohols small organic molecule fluorescence sensing materials. The application operation is simple, the cost is low, the respond is quick, and the obvious fluorescence response for methanol, ethanol, isopropanol , n-propanol under the liquid phase and the gas phase can be achieved.

The present invention relates to polymerisable fluorescent functional monomers in the structure as shown, and they have strong fluorescent reaction and part capable of reacting with biomacromolecular protein and non-covalent bond of DNA mutually. They may be polymerized with hydrophilic monomer to obtain fluorescent amphiphilic polymer with excellent biocompatibility, medicine carrying capacity and target administration capacity, and the polymer may find wide application foreground in high sensitivity fluorescent tracing detection technology, biomacromolecule separation and distinction, biosensor, medical diagnosis, medicine conveying, target material and other aspects. The present invention has reasonable synthesis path, simple synthesis process, easy-to-obtain material and high application value.

The invention discloses a rhodamine 6G hydrazide derivative, a preparation method of the derivative and an application of the derivative, and a method for carrying out fluorescence analysis on hypochlorous acid by using the derivative as a fluorescence probe. The rhodamine 6G hydrazide derivative is selectively reacted with hypochlorous acid to rapidly generate an obvious fluorescence enhancement effect. The rhodamine 6G hydrazide derivative disclosed by the invention is capable of realizing high-sensitivity and high-selectivity quantitative detection on trace hypochlorous acid in water and cells, and wide in application prospect in the fields of environment, biology and the like.

The invention discloses a rare earth organic framework material for fluorescence detection of trace water and a preparation method of the rare earth organic framework material. The rare earth organic framework material is an orderly microporous crystalline material, and the general structural formula of the material is [(CH3)2NH2]2[Ln6(mu3-OH)8(L-NH2)6(H2O)6]*(G)7, wherein Ln in the formula is Eu<3+>, Tb<3+> or Gd<3+>, L-NH2 is a chainlike phthalic acid organic ligand with an amino group function group and G represents a solvent in pores. The rare earth organic framework material is synthesized with a solvothermal method, the synthesis method adopts a simple process, the condition is mild, the yield is higher, and the product stability is good. The rare earth organic framework material has the properties of characteristic emission of rare earth and fluorescence of the ligand, the specific value of two emission peaks and the content of water in the solvent are in a better linear relation, and ratio type detection of trace water can be realized; the light color remarkably changes with the change of the water content, and the material can be used for colorimetric type detection of trace water. The sensitivity of a probe is high, the response speed is high, the detection limit is low, and the material can be recycled and is expected to be used practically in fields of detection and monitoring of trace water.

The invention discloses N-(2,4-dinitrophenyl)-rhodamine B hydrazide and a preparation method and an application of the N-(2,4-dinitrophenyl)-rhodamine B hydrazide. The rhodamine B derivative provided by the invention is N-(2,4-dinitrophenyl)-rhodamine B hydrazide, the structural formula of which is I. The N-(2,4-dinitrophenyl)-rhodamine B hydrazide, is colorless and free from fluorescence, but can selectively react with Cu<2+> ions in chromogenic and fluorescent opening reaction. The colorless substance generates a system with excellent optical performance, so that the detection sensitivity and selectivity of Cu<2+> ions are greatly improved. Therefore, the N-(2,4-dinitrophenyl)-rhodamine B hydrazide is appropriate for high selectivity and high sensitivity detection for the Cu<2+> ions. Detection can be carried out by a light absorption spectrum and a fluorescence spectrum. (Formula I is shown in the specification).