Rhodamine 6g hydrazide derivative, its preparation method, application and method for fluorescent analysis of hypochlorous acid as a fluorescent probe

A hydrazide derivative, fluorescence analysis technology, applied in the direction of fluorescence/phosphorescence, biological testing, material excitation analysis, etc., can solve the problems of cumbersome process, complicated technology, restricting practical application, etc., to achieve wide application prospects, high sensitivity, fluorescence Detecting the effect of signal stabilization

Inactive Publication Date: 2016-06-29
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the synthesis process of these reagents is cumbersome and technically complex, which restricts their practical application in environmental analysis and biological analysis.

Method used

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  • Rhodamine 6g hydrazide derivative, its preparation method, application and method for fluorescent analysis of hypochlorous acid as a fluorescent probe
  • Rhodamine 6g hydrazide derivative, its preparation method, application and method for fluorescent analysis of hypochlorous acid as a fluorescent probe
  • Rhodamine 6g hydrazide derivative, its preparation method, application and method for fluorescent analysis of hypochlorous acid as a fluorescent probe

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Embodiment 1

[0032] The preparation method of rhodamine 6G hydrazide derivative of the present invention comprises the following steps:

[0033] 1) Weighing a certain amount of rhodamine 6G, ethanol and hydrazine monohydrate with a mass concentration of 80%, and dissolving rhodamine 6G in ethanol, then adding hydrazine monohydrate dropwise, and then refluxing and filtering to obtain After precipitation, washing and drying, rhodamine 6G hydrazide was obtained as a pink solid, wherein the ratio of rhodamine 6G, ethanol and hydrazine monohydrate was 1.0g: 20mL: 3mL;

[0034] 2) Take a certain amount of 1-naphthoyl chloride and acetonitrile, then dissolve 1-naphthoyl chloride in acetonitrile to obtain an acetonitrile solution of 1-naphthoyl chloride, wherein 1-naphthalene in the acetonitrile solution of 1-naphthoyl chloride The ratio of formyl chloride to acetonitrile is 100 μL: 15 mL;

[0035] 3) Weigh a certain amount of rhodamine 6G hydrazide and acetonitrile, then dissolve rhodamine 6G hy...

Embodiment 2

[0041] The preparation method of rhodamine 6G hydrazide derivative of the present invention comprises the following steps:

[0042] 1) Weighing a certain amount of rhodamine 6G, ethanol and hydrazine monohydrate with a mass concentration of 80%, and dissolving rhodamine 6G in ethanol, then adding hydrazine monohydrate dropwise, and then refluxing and filtering to obtain After precipitation, washing and drying, rhodamine 6G hydrazide was obtained as a pink solid, wherein the ratio of rhodamine 6G, ethanol and hydrazine monohydrate was 1.0g: 30mL: 2mL;

[0043] 2) Take a certain amount of 1-naphthoyl chloride and acetonitrile, then dissolve 1-naphthoyl chloride in acetonitrile to obtain an acetonitrile solution of 1-naphthoyl chloride, wherein 1-naphthalene in the acetonitrile solution of 1-naphthoyl chloride The ratio of formyl chloride to acetonitrile is 90 μL: 13 mL;

[0044] 3) Weigh a certain amount of rhodamine 6G hydrazide and acetonitrile, then dissolve rhodamine 6G hydra...

Embodiment 3

[0050] The preparation method of rhodamine 6G hydrazide derivative of the present invention comprises the following steps:

[0051] 1) Weighing a certain amount of rhodamine 6G, ethanol and hydrazine monohydrate with a mass concentration of 80%, and dissolving rhodamine 6G in ethanol, then adding hydrazine monohydrate dropwise, and then refluxing and filtering to obtain After precipitation, washing and drying, rhodamine 6G hydrazide was obtained as a pink solid, wherein the ratio of rhodamine 6G, ethanol and hydrazine monohydrate was 1.0g: 25mL: 4mL;

[0052] 2) Take a certain amount of 1-naphthoyl chloride and acetonitrile, then dissolve 1-naphthoyl chloride in acetonitrile to obtain an acetonitrile solution of 1-naphthoyl chloride, wherein 1-naphthalene in the acetonitrile solution of 1-naphthoyl chloride The ratio of formyl chloride to acetonitrile is 115 μL: 11 mL;

[0053] 3) Weigh a certain amount of rhodamine 6G hydrazide and acetonitrile, then dissolve rhodamine 6G hy...

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Abstract

The invention discloses a rhodamine 6G hydrazide derivative, a preparation method of the derivative and an application of the derivative, and a method for carrying out fluorescence analysis on hypochlorous acid by using the derivative as a fluorescence probe. The rhodamine 6G hydrazide derivative is selectively reacted with hypochlorous acid to rapidly generate an obvious fluorescence enhancement effect. The rhodamine 6G hydrazide derivative disclosed by the invention is capable of realizing high-sensitivity and high-selectivity quantitative detection on trace hypochlorous acid in water and cells, and wide in application prospect in the fields of environment, biology and the like.

Description

technical field [0001] The present invention relates to a derivative for detecting hypochlorous acid, its preparation method, application and detection method, in particular to a rhodamine 6G hydrazide derivative, its preparation method, application and its use as a fluorescent probe to perform fluorescence on hypochlorous acid method of analysis. Background technique [0002] Hypochlorous acid (HOCl) is a strong oxidizing agent that can play an antimicrobial role in environmental water treatment processes and living organisms. In daily life, hypochlorous acid has been widely used as a tap water disinfectant because of its good bactericidal effect, low price and convenient use. However, high-concentration hypochlorous acid solution is highly irritating, harmful to the respiratory system, and easily reacts with organic matter in water to generate serious carcinogens such as chloroform and carbon tetrachloride, which are potentially harmful to human health. In living organis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/107C09K11/06G01N21/64
CPCC07D491/107C09K11/06C09K2211/1088G01N21/64G01N33/52
Inventor 张祯邓成权孟列素颜晓梅
Owner XI AN JIAOTONG UNIV
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