32results about How to "Solve hard-to-get puzzles" patented technology

The invention belongs to the field of technical methods for preventing tumors, and in particular relates to a tumor microenvironment induced cancerous human umbilical vein endothelial cell vaccine andan application thereof in resisting angiogenesis of colon carcinoma. The vaccine is prepared by the following steps: collecting supernatant of CT26 cells, inducing HUVEC cells via the CT26 cell supernatant, collecting the HUVEC cells, immobilizing glutaraldehyde and the like. According to the invention, tumor vessel endothelial cells function as a target in tumor immnuotherapy, belonging to active immunotherapy. The cancerous HUVEC vaccine is prepared through inducing by an in-vitro simulated tumor microenvironment. Based upon preliminary experimental results, it is indicated that the prepared cancerous HUVEC vaccine can cause a more effective action in inhibiting tumor angiogenesis, and subsequently, an effect of resisting colon carcinoma growth can be developed; therefore, a relativelygood application effect can be achieved; and meanwhile, a new possibility is also provided for the application of a treatment method based on resisting tumor angiogenesis.

The invention relates to a long-focus transmission-type quasi telecentric three-linear-array stereoscopic mapping optical system. The long-focus transmission-type quasi telecentric three-linear-array stereoscopic mapping optical system comprises three optical subsystems with completely consistent structure forms, wherein each optical subsystem comprises a front lens group (1), a diaphragm (6), a middle lens group (2), a rear lens group (3), a transflective prism (4) and a receiving image surface (5), all of which are sequentially arranged along the light ray incidence direction, the caliber of a radiation beam of a mapping target is regulated after the radiation beam passes through the front lens group (1), the radiation beam is full of the diaphragm (6) to exit, an angle amplification ratio is regulated after the outgoing beam passes through the middle lens group (2) and the rear lens group (3), and the outgoing beam exits to the transflective prism (4), so that the outgoing beam is divided and then is imaged on the receiving image surface (5). The optical system disclosed by the invention has the advantages of long-focus transmission type, quasi telecentricity, zero distortion, good stability of element of interior orientation, high optical machine structure integration degree and the like, and can realize higher-proportion stable mapping function in the terrain and the geomorphology of a target scene through the reasonable arrangement of a front-vision camera, a positive-vision camera and a back-vision camera.

The invention discloses a three-dimensional roughness description method for a rock mass structural surface based on borehole camera technology. The method comprises the steps of obtaining a hole walldeveloped panoramic view; obtaining the contour line of the lower wall surface of the rock mass structural surface; fitting a structural surface fitted line, and obtaining the reference plane of thelower wall surface of the rock mass structural surface, and the inclination orientation and the inclination angle of the rock mass structural surface; acquiring a contour line developed curve; acquiring a spliced contour line; obtaining the reference contour line of the set sampling length and the set sampling precision; according to the fractal dimension of each reference contour line segment, forming a structural surface roughness coefficient rose diagram and describing the three-dimensional roughness of the rock mass structural surface based on the same. The method can well describe the three-dimensional roughness information of the rock mass structural surface, and solves the problem that the three-dimensional roughness information of a deep rock mass structural surface is difficult toobtain.

The invention discloses a Peziza repanda Pers. strain separation method which is characterized by comprising the following steps: preparing a culture medium, respectively pouring the culture medium into culture bottles, capping, autoclaving, cooling, carrying out preculture in a culture chamber for 3 days, and carrying out material inoculation if no infectious microbe pollution occurs; flushing the material with tap water, sterilizing, absorbing water with sterile filter paper, cutting the required position, taking a 5mm block, inoculating the material to the obtained culture bottles, and culturing; putting the culture bottles in a culture chamber with the temperature of 25 DEG C or so, and culturing for 7-10 days to form the callus; and dicing the formed callus, inoculating to a fresh PDA (potato dextrose agar) culture medium, putting the PDA culture medium in a constant-temperature environment of 25 DEG C or so, and culturing, wherein the hyphae can grow out after 3-5 days, and can be full of the test tube after 15 days. The method solves the problem that the Peziza repanda Pers. strain is difficult to obtain, and obtains the high-purity Peziza repanda Pers. strain.

The invention discloses an aerial moving target sequence image simulation method based on a remote sensing platform, which can enable features of the extracted moving target to be more real. A pure black base map is generated; an already-set target track is added to the map, and the initial point of the target track serves as the current point M<g>; a moving target template is made and a background image is selected, a point M<g+1> with a distance of V1 is selected, and the heading angle at the point M<g+1> is calculated; the moving target template is placed at the point M<g+1> and is rotated to be consistent with the heading angle at the point M<g+1> and is then saved; and the center point of the well-saved moving target template is placed at the point M<g+1> in the background image; and corresponding processing is carried out on pixels whose gray values are no smaller than 0 in the moving target template, and a simulation map sequence for the moving target can be further acquired.