32results about How to "Solve hard-to-get puzzles" patented technology

The invention belongs to the field of technical methods for preventing tumors, and in particular relates to a tumor microenvironment induced cancerous human umbilical vein endothelial cell vaccine andan application thereof in resisting angiogenesis of colon carcinoma. The vaccine is prepared by the following steps: collecting supernatant of CT26 cells, inducing HUVEC cells via the CT26 cell supernatant, collecting the HUVEC cells, immobilizing glutaraldehyde and the like. According to the invention, tumor vessel endothelial cells function as a target in tumor immnuotherapy, belonging to active immunotherapy. The cancerous HUVEC vaccine is prepared through inducing by an in-vitro simulated tumor microenvironment. Based upon preliminary experimental results, it is indicated that the prepared cancerous HUVEC vaccine can cause a more effective action in inhibiting tumor angiogenesis, and subsequently, an effect of resisting colon carcinoma growth can be developed; therefore, a relativelygood application effect can be achieved; and meanwhile, a new possibility is also provided for the application of a treatment method based on resisting tumor angiogenesis.

The invention discloses a hyperspectral fusion computing imaging method and system, the invention includes low-resolution hyperspectral image X lr Perform subspace representation to obtain low-rank spectral basis S and low-resolution subspace coefficient A lr ; The low-resolution subspace coefficient A lr After upsampling, it is stacked with the multispectral image Y and input to the pre-trained convolutional neural network to obtain the high-resolution subspace coefficient A; the high-resolution subspace coefficient A and the low-rank spectral basis S are fused to obtain the fused high-resolution hyperspectral Image X. The invention can effectively achieve high-precision quantitative establishment and solution of imaging models of hyperspectral and multispectral images, and generate training data from images to be fused through the established imaging models, solve the problem of insufficient training data, and integrate convolutional neural networks and sub- The combination of spatial representation models reduces network parameters and improves learning efficiency. It has the advantages of lightweight models, high computational efficiency, and high fusion accuracy.

The invention relates to a mobile robot variable pesticide applying intelligent decision-making method and a system. The mobile robot variable pesticide applying intelligent decision-making system comprises a camera, an ultrasonic sensor, a flow sensor and a computer, the camera is used for sending an acquired greenhouse crop image to the computer through a universal serial bus (USB) interface, the ultrasonic sensor is used for measuring the distance of a sprayed target and sending the information of the distance to the computer, the flow sensor is used for measuring actual sprayed pesticide flow, the computer is connected with the camera, the ultrasonic sensor and the flow sensor, a fuzzy neural network decision-making device is configured in the computer, the fuzzy neural network decision-making device is used for obtaining target area, spraying distance and plant diseases and insect pests grade information, preset pesticide applying amount is calculated according to the obtained target area, spraying distance and plant diseases and insect pests grade information, a control amount is calculated and output according to the preset pesticide applying amount, the actual pesticide applying amount is obtained, the actual pesticide applying amount is compared with the preset pesticide applying amount, and the control amount is adjusted.

The invention discloses a power penetration flat-plate anchor test device for a supergravity centrifuge. The test device consists of five parts: the test model box, the magnetic induction positioning system, the anchor release device, the load measurement device and the dynamic penetration plate anchor; there is a test foundation in the test model box, and a The slide rail of the model box is equipped with an anchor release device and a loading measurement device; the magnetic induction positioning system is installed on the anchor plate of the dynamic penetration flat plate and the test model box. The invention solves the problem that it is difficult to obtain the motion information of the anchor body due to the invisibility of the soil, and can accurately and effectively perform the power penetration plate anchor test of the supergravity centrifuge.

Disclosed is a new method for obtaining ornamental arrowroot tissue culture germfree systems, which is an improved method for obtaining the germfree systems through the secondary transition planting of tissue culture female parent plant and the comprehensive sterilization of explants, by adding the sanitizers in the culture medium for co-cultivation; the plant tissue culture is made under sterile conditions; the process comprises the following steps: the female parent plant goes through secondary transition planting, namely, the female parent plant is cultivated in perlite or ceramic particles for 2-3 weeks and then removed into water for water culture to induce new leaves with less endophytes, and then the new leaves are taken as explants for tissue culture; the material goes through preliminary disinfection and low-temperature pretreatment; two disinfectant fungicides of different dosage are added into the induced culture medium to prepare the induced culture medium which can restrain bacteria; and the explants are treated through surface disinfection with an integrated approach. The method is unique, simple, practical and highly valued, which can obtain a large amount of aseptic systems in a short time, so as to save time, labor and expenses. The method enables the yield rate of the aseptic explants to reach 65-70%.

The invention relates to a long-focus transmission-type quasi telecentric three-linear-array stereoscopic mapping optical system. The long-focus transmission-type quasi telecentric three-linear-array stereoscopic mapping optical system comprises three optical subsystems with completely consistent structure forms, wherein each optical subsystem comprises a front lens group (1), a diaphragm (6), a middle lens group (2), a rear lens group (3), a transflective prism (4) and a receiving image surface (5), all of which are sequentially arranged along the light ray incidence direction, the caliber of a radiation beam of a mapping target is regulated after the radiation beam passes through the front lens group (1), the radiation beam is full of the diaphragm (6) to exit, an angle amplification ratio is regulated after the outgoing beam passes through the middle lens group (2) and the rear lens group (3), and the outgoing beam exits to the transflective prism (4), so that the outgoing beam is divided and then is imaged on the receiving image surface (5). The optical system disclosed by the invention has the advantages of long-focus transmission type, quasi telecentricity, zero distortion, good stability of element of interior orientation, high optical machine structure integration degree and the like, and can realize higher-proportion stable mapping function in the terrain and the geomorphology of a target scene through the reasonable arrangement of a front-vision camera, a positive-vision camera and a back-vision camera.

The invention discloses an X-ray grazing-incidence reflecting-mirror effective area calibration method. The method comprises the following steps of adjusting a diaphragm aperture and a distance between the diaphragm aperture and an X-ray tube, and starting a narrow-beam X-ray source; measuring an X-ray narrow beam intensity; aligning an X-ray grazing-incidence reflecting mirror and the narrow-beam X-ray source; using a two-dimensional translation platform to scan the X-ray grazing-incidence reflecting mirror; measuring a photon number C of a reflecting-mirror focal point; calculating an on-axis effective area of the X-ray grazing-incidence reflecting mirror; and rotating the X-ray grazing-incidence reflecting mirror, repeating the step4 to the step6 and then acquiring an off-axis effective area of the X-ray grazing-incidence reflecting mirror. In the invention, through the adjustable aperture diaphragm, a diameter of a X-ray beam is limited, a high-collimation X-ray calibration beam is realized, and a problem that a traditional high-collimation X-ray calibration beam is difficult to acquire is solved; and through arranging the two-dimensional translation platform, a technical effect that a narrow beam scans and calibrates an effective area of the grazing-incidence reflecting mirror is reached and a defect that calibration accuracy is not high in a traditional method is overcome.

The invention discloses a three-dimensional roughness description method for a rock mass structural surface based on borehole camera technology. The method comprises the steps of obtaining a hole walldeveloped panoramic view; obtaining the contour line of the lower wall surface of the rock mass structural surface; fitting a structural surface fitted line, and obtaining the reference plane of thelower wall surface of the rock mass structural surface, and the inclination orientation and the inclination angle of the rock mass structural surface; acquiring a contour line developed curve; acquiring a spliced contour line; obtaining the reference contour line of the set sampling length and the set sampling precision; according to the fractal dimension of each reference contour line segment, forming a structural surface roughness coefficient rose diagram and describing the three-dimensional roughness of the rock mass structural surface based on the same. The method can well describe the three-dimensional roughness information of the rock mass structural surface, and solves the problem that the three-dimensional roughness information of a deep rock mass structural surface is difficult toobtain.

The invention relates to a method for measuring a pH value of carbonated concrete pore solution, belonging to the technical field of civil engineering materials. The method for measuring the pH value of the carbonated concrete pore solution provided by the invention comprises the following steps of: producing thin concrete test pieces, accelerating carbonation, filter pressing the concrete pore solution, preparing suspending liquid by concrete powder and the like. The pH value of the concrete pore solution is calculated by measuring the pH value of the concrete pore solution in different carbonation degrees and the pH value of the carbonated concrete powder suspending liquid, establishing the relationship between the pH value pH value of the concrete pore solution in different carbonationdegrees and the pH value of the suspending liquid, and measuring the pH value of the concrete suspending liquid. The method for measuring the pH value of the carbonated concrete pore solution provided by the invention is simple in equipment, low in cost, excellent in effect and the like; and the method is suitable for durability test research work such as concrete carbonation, pH measurement and the like.

The invention discloses a Peziza repanda Pers. strain separation method which is characterized by comprising the following steps: preparing a culture medium, respectively pouring the culture medium into culture bottles, capping, autoclaving, cooling, carrying out preculture in a culture chamber for 3 days, and carrying out material inoculation if no infectious microbe pollution occurs; flushing the material with tap water, sterilizing, absorbing water with sterile filter paper, cutting the required position, taking a 5mm block, inoculating the material to the obtained culture bottles, and culturing; putting the culture bottles in a culture chamber with the temperature of 25 DEG C or so, and culturing for 7-10 days to form the callus; and dicing the formed callus, inoculating to a fresh PDA (potato dextrose agar) culture medium, putting the PDA culture medium in a constant-temperature environment of 25 DEG C or so, and culturing, wherein the hyphae can grow out after 3-5 days, and can be full of the test tube after 15 days. The method solves the problem that the Peziza repanda Pers. strain is difficult to obtain, and obtains the high-purity Peziza repanda Pers. strain.

The invention discloses an aerial moving target sequence image simulation method based on a remote sensing platform, which can enable features of the extracted moving target to be more real. A pure black base map is generated; an already-set target track is added to the map, and the initial point of the target track serves as the current point M<g>; a moving target template is made and a background image is selected, a point M<g+1> with a distance of V1 is selected, and the heading angle at the point M<g+1> is calculated; the moving target template is placed at the point M<g+1> and is rotated to be consistent with the heading angle at the point M<g+1> and is then saved; and the center point of the well-saved moving target template is placed at the point M<g+1> in the background image; and corresponding processing is carried out on pixels whose gray values are no smaller than 0 in the moving target template, and a simulation map sequence for the moving target can be further acquired.

The invention relates to a forming die and method for magnesium alloy thin webbed supporting fork forgings, in particular to an isothermal die forging forming die and method for a rare earth magnesium alloy thin webbed supporting fork. The problems that rare earth magnesium alloy thin webbed supporting fork forgings are prepared through conventional forging, blank manufacturing and forming are hard, forming defects are prone to occurring, the structure performance of the magnesium alloy forgings is hard to control, and the performance is low are solved. The forming method comprises the main steps that 1, blank manufacturing is carried out; 2, forging forming is carried out; 3, cooling is carried out; 4, primary acid pickling is carried out; 5, damage repairing is carried out; 6, final forging is carried out; 7, secondary acid pickling is carried out, wherein a magnesium alloy forging obtained in the sixth step is subjected to acid pickling, graphite on the surface is cleaned away, and a formed forging with the treated surface is obtained; and 8, the formed forging with the treated surface obtained in the seventh step is put into an aging oven to be subjected to aging treatment, and the finished rare earth magnesium alloy thin webbed supporting fork is manufactured. The forming die and method are used for preparing of the magnesium alloy thin webbed supporting fork.

The invention provides a model test device for simulating the long-term land subsidence in a highly-dense area under a complex vibration environment, and belongs to the technical field of urban underground engineering disaster prevention and reduction and land subsidence geological disasters. The model test device for simulating the long-term land subsidence in a highly-dense area under a complexvibration environment includes a model box, a stratum system, a tunnel system, a high-speed railway roadbed system, a vibration excitation system, a sensing system, a ground dense building group, a high-speed rotating system, and a karst cave system. The device can simulate the long-term land subsidence caused by the joint vibration of subways and high-speed railways in a dense building group area, can accurately simulate the long-term land subsidence under the effect of high-speed rotating centrifugal force in a complex environment, can quantitatively monitor the influence of the reinforcement of existing karst caves over and under an operating tunnel on the long-term land subsidence, and is low in experimental cost and reliable in data.