Peziza repanda Pers. strain separation method

Abstract

The invention discloses a Peziza repanda Pers. strain separation method which is characterized by comprising the following steps: preparing a culture medium, respectively pouring the culture medium into culture bottles, capping, autoclaving, cooling, carrying out preculture in a culture chamber for 3 days, and carrying out material inoculation if no infectious microbe pollution occurs; flushing the material with tap water, sterilizing, absorbing water with sterile filter paper, cutting the required position, taking a 5mm block, inoculating the material to the obtained culture bottles, and culturing; putting the culture bottles in a culture chamber with the temperature of 25 DEG C or so, and culturing for 7-10 days to form the callus; and dicing the formed callus, inoculating to a fresh PDA (potato dextrose agar) culture medium, putting the PDA culture medium in a constant-temperature environment of 25 DEG C or so, and culturing, wherein the hyphae can grow out after 3-5 days, and can be full of the test tube after 15 days. The method solves the problem that the Peziza repanda Pers. strain is difficult to obtain, and obtains the high-purity Peziza repanda Pers. strain.

Description

technical field [0001] The invention relates to the field of edible fungi, in particular to a method for isolating the strains of the fungi. Background technique [0002] Conventional large-scale edible fungus strains are obtained by tissue separation, that is, small pieces of tissue are taken out from the fruit body and placed in PDA medium to cultivate mycelia. The obtained mycelia are used as strains for production, germplasm preservation, and functional Substance extraction and other purposes. The genus of the genus Sclerophylliaceae, Sclerophyllaceae, and Sclerotium genus is initially cup-shaped, then stretches, and the edge is completely rounded and wavy inwardly, yellowish-brown to brown, and the outside is nearly white and rough. The meat of the pleinosa is crisp, the water content is as high as about 95%, and the fruiting body layer is thin. It is impossible to obtain high-purity strains by traditional tissue separation methods. The acquisition of high-purity stra...

AI Technical Summary

Problems solved by technology

The meat of the pleinosa is crisp, the water content is as high as about 95%, and the fruiting body layer is thin. It is impossible to obtain high-purity strains by traditional tissue separation methods.