Method for screening anti-black rot mutant plants with cabbage free microspores

Abstract

The invention discloses a method for screening anti-black rot mutant plants with cabbage free microspores. The method comprises the following steps: (1) separating and purifying black rot pathogenic bacteria; (2) roughly extracting toxin of black rot pathogenic bacteria; (3) dissociating and purifying microspores; (4) inducing a disease-resistant mutation embryoid; (5) differentiating adventitious buds of a disease-resistant mutant by the embryoid; and (6) performing rooting culture on adventitious buds. According to the invention, the disease-resistant mutant raw material is firstly taken from a haploid cell or a double haploid cell, extensive variation types can be generated, and the abundant hereditary basis can be supplied for disease-resistant selection; a mutant always happens at cell level and is applied to a microspore mutant for easily selecting a recessive gene and polygene homozygous dominance mutant; the mutant can be doubled through ploidy, so as to quickly acquire a homozygous disease-resistant material; the black rot pathogenic bacteria rough toxin is taken as a selective agent and is greatly beneficial to the promotion of gene aberration rate and the expansion of variation spectrum.

Description

technical field [0001] The invention belongs to the technical field of cabbage disease-resistant breeding, and relates to a method for creating cabbage disease-resistant regenerated plants by using haploid or double haploid cell mutants by using cabbage free microspore culture and additional cabbage black rot crude toxin screening. Background technique [0002] Cabbage is one of the main vegetable crops in the world, and the occurrence of diseases in cultivation has become the main restrictive factor that endangers the development of the industry. Cabbage virus disease (Virus) in European and American countries, Chinese cabbage black rot (Blackrot), Japanese cabbage verticillium wilt (Verticillium wilt) and Korean cabbage wilt (Blight) are all major diseases that endanger the growth of cabbage at present. Therefore, breeding research on the main diseases of cabbage in various countries in the world has become the main goal of disease-resistant breeding. The first-generati...

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Problems solved by technology

The first two approaches adopt conventional breeding methods, and the screening of antigenic materials whose disease-resistant genes are not easy to be completely homozygous will lead to poor resistance, or gene homozygosity will cause excessive breeding time and lead to poor breeding efficiency; the latter is being explored by experts and scholars in recent years.

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