The invention discloses an in-situ online detection device for phytoplanktons based on
photosynthetic pigment fluorescence. The in-situ online detection device comprises an LED (
Light Emitting Diode) annular
light source, a cylindrical
water sample pool, a fluorescent detection module, a microcontrol circuit module, a casing and a bottom cover. According to the in-situ online detection device disclosed by the invention, by using
fluorescence characteristics of photosynthetic pigments of different phytoplanktons, measurement of living
fluorescence intensity and
chlorophyll fluorescence induction kinetic parameters of the phytoplanktons under the action of exciting light with four wavelengths is realized; meanwhile, the
biomass and physiological activity of the phytoplanktons can be reflected; the in-situ online detection device is suitable for real-time monitoring and early warning of
eutrophication conditions in key water areas. According to the in-situ online detection device disclosed by the invention, the annular
light source is adopted, the
utilization rate of a
light source and the illumination uniformity are considered, and a main
control circuit takes STM32L151 as a core, so that the device can carry out long-term high-performance operation
underwater; the in-situ online detection device is provided with the openable and closable bottom cover, so that in-situ
water sampling underwater can be realized, the interference of ambient light of a
water body and water current fluctuation is overcome, and further the accuracy of in-situ detection is improved.