The invention discloses a three-dimensional hollow
light spot generating method and a three-dimensional hollow
light spot generating device. The three-dimensional hollow
light spot generating device comprises a
laser, a single mode
fiber, a collimating lens, a first polarizing
beam splitter prism, a plurality of light turning assemblies, two phase modulators, a second polarizing
beam splitter prism, a 1 / 4 wave plate, a microobjective, a sample surface and a medium film reflecting lens. The three-dimensional hollow light spot generating method comprises the following steps; performing different
phase modulation on vertical polarized light and parallel polarized light; combining the two modulated light beams, and converting the combined
light beam into two circuit polarized lights by the same 1 / 4 wave plate; projecting the two circuit polarized lights by the microobjective to penetrate through the sample surface, and reflecting the two circuit polarized lights by the medium film reflecting lens; interfering the reflected light irradiated to the sample surface with projecting light to form a transverse hollow light spot and an axial hollow light spot respectively; and superposing the light intensities of the two hollow light spots to form a three-dimensional hollow light spot, wherein the transverse size of the three-dimensional hollow light spot is 0.56 wavelengths and the axial size of the three-dimensional hollow light spot is 0.44 wavelengths. The three-dimensional hollow light spot generating method and the three-dimensional hollow light spot generating device can be applied to super-resolution microscopic equipment such as a
stimulated emission depletion
microscope and are used for realizing three-dimensional super-resolution
microscopy.