The invention discloses a culture method for a stable and homozygous tobacco
chromosome single fragment substitution line. The culture method comprises the steps that tobacco
germplasm Y3 with a good comprehensive form serves as a recurrent parent, the high-quality tobacco variety K326 and the
disease-resistant cigar variety Beinhart 1000-1 serve as donor parents, the technologies of hybridization,
backcrossing and
molecular marker-assisted selection are adopted, molecular markers obtained through an established tobacco
genetic linkage map are used for carrying out assisted selection on the
backcrossing second generation and the subsequent
backcrossing generations thereof, and the self-crossing
first generation and the later generations thereof,
chromosome substitution fragment features are identified, and the stable and homozygous tobacco
chromosome single fragment substitution line is obtained. By means of the culture method, the genetic basis of tobacco varieties can be expanded,
permeation of exogenous good
germplasm to culture varieties can be rapidly achieved, a convenient, practical, scientific and efficient approach is provided for the high-quality and
disease resistance
polymerization oriented improvement on tobacco, the selectivity of the breeding process is improved, the breeding efficiency is improved, and the culture process of new high-quality and
disease resistance tobacco varieties and the seed industrialization process are accelerated.