Molecular marker for soybean bemisia tabaci resistance breeding and marking method of molecular marker
A molecular marker, Bemisia tabaci technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Gene has not been reported and other problems, to achieve the effect of reducing the workload of high-generation breeding selection, reducing the workload of selection, and realizing fine positioning
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[0019] Embodiment 1, 1. a kind of molecular marker method that is used for soybean anti-bemisia tabaci breeding, comprises the following steps:
[0020] 1) Distribution of soybean RIL populations resistant to whitefly: Huapidou and Qihuang 26 high-generation RIL populations were planted for two consecutive years, and soybean RIL populations were identified for resistance to whitefly, repeated twice; Analyze the average;
[0021] 2) QTL mapping of soybean resistance to whitefly: using the high-density genetic map constructed by SLAF-seq technology to map the QTL of soybean RIL population resistance to whitefly, and obtain two QTLs related to soybean resistance to whitefly location.
[0022] 2. Identification of Bemisia tabaci resistance in soybean RIL population
[0023] In 2016, 149 soybean RIL populations were identified for resistance to whitefly, repeated twice. Analysis of the 2 averages showed that (Table 1, figure 1 ): The resistance of RIL populations to Bemisia tab...
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