Molecular markers, screening methods, breeding methods and applications for screening soybean materials

Abstract

The invention discloses a molecular marker for screening soybean materials, a screening method, a breeding method and applications. The molecular markers include InDel markers or / and dCAPS markers. The step of using the aforementioned molecular markers to screen soybean high-protein materials includes: selecting the InDel Mark as a primer pair; use the InDel-marked primer pair to select one or more soybean variety DNAs for first PCR amplification; perform the first electrophoresis detection on the first PCR amplification product, and the first electrophoresis detection band The soybean varieties presented as high bands are low-protein varieties, and the soybean varieties presented as low bands in the first electrophoresis detection band are high-protein varieties. Based on the whole genome resequencing and QTL positioning technology, the present invention develops high-protein InDel and dCAPS with clear targets, and uses the combination of InDel and dCAPS to mark soybean high-protein resources in the future and molecular marker-assisted breeding for high-protein, agronomic and Breeding materials or varieties with excellent quality traits provide new ways.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a molecular marker for screening soybean high-protein materials, a method for screening soybean high-protein materials using the primers, and a method for using the primers to screen target strains for breeding. Background technique [0002] Soybean grains are rich in protein, accounting for 31% to 55% of grain dry matter, and are one of the main sources of human food protein. Its protein output accounts for two-thirds of the protein output of protein crops, and what is even more rare is that it is rich in a variety of essential amino acids. The World Health Organization defines soybean protein as a first-class protein. At the same time, soybean protein has good functional properties and can be used in a variety of food systems to improve product quality. [0003] At present, protein-related QTL loci have been found on all 20 chromosomes of soybean, among which 213 protei...

AI Technical Summary

Problems solved by technology

[0006] Most of the currently developed molecular markers are based on linkage analysis and cannot directly target the target gene. The separation and recombination of genes will cause the loss of marker information and cause certain difficulties in the selection of target traits.

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