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Dactylis glomerata vernalization gene DgPAPS4 and application thereof

A technology of gene and Dactylis, which is applied in the field of cloning the primer set of the gene from plant DNA, can solve the problems of large variation in Dracula flowering period, lack of Dracula species, and limited application, so as to reduce the breeding workload and improve breeding efficiency , the effect of shortening the breeding period

Active Publication Date: 2022-08-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the flowering period of Dactylis chinensis varies greatly, and there is a lack of varieties of Dactylis chinensis suitable for flowering period in actual production, which seriously limits its application in production
Years of research on the flowering period of Dactylis chinensis has been conducted with little effect

Method used

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  • Dactylis glomerata vernalization gene DgPAPS4 and application thereof
  • Dactylis glomerata vernalization gene DgPAPS4 and application thereof
  • Dactylis glomerata vernalization gene DgPAPS4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This example describes a vernalization gene DgPAPS4 that can be expressed in Duckweed to regulate the flowering time of Duckweed. The vernalization gene DgPAPS4 comprises a nucleotide sequence selected from the group consisting of:

[0036] A. The nucleotide sequence shown in Sequence Listing Seq ID NO.1.

[0037] B. A nucleotide sequence complementary to the sequence described in A. Or nucleotide sequences with more than 70% homology, such as nucleotide sequences with more than 80% homology, nucleotide sequences with more than 90% homology, nucleotide sequences with more than 95% homology .

[0038] C. The nucleotide sequence encoding the amino acid sequence shown in Sequence Listing Seq ID NO.2.

Embodiment 2

[0040] This example describes a protein whose amino acid sequence is shown in Seq ID NO.2. The sequence is encoded by DgPAPS4(A), the vernalization gene of Duckweed in Example 1.

Embodiment 3

[0042] This example describes the primer pair used for cloning the vernalization gene PAPS4, the base sequences of the primer pair are respectively the upstream primer F shown in Sequence Listing Seq ID NO.3: 5'-atggtaccatgacgacctctaataagccgatat-3', Sequence Listing Downstream primer R shown in Seq ID NO.4: 5'-atcgctagcctacaaactaatgcatggccatttc-3'. Using this primer pair, the nucleotide sequence shown in Seq ID NO. 1 of the sequence listing can be extracted from the DNA of Duckweed.

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Abstract

The invention discloses a dactylis glomerata vernalization gene DgPAPS4 and application thereof, the vernalization gene DgPAPS4 is expressed in a plant to adjust the flowering time of the plant, the gene DgPAPS4 comprises a nucleotide sequence selected from the following groups: A, a nucleotide sequence shown in a sequence table SeqID NO.1; b, a nucleotide sequence complementary to the sequence of A, or a nucleotide sequence with more than 70% of homology; c, a nucleotide sequence of an amino acid sequence as shown in Seq ID NO.2 in a coding sequence table; the invention also provides an expression vector containing the vernalization gene DgPAPS4, a host cell containing the overexpression vector, and an application of the vernalization gene DgPAPS4. The vernalization gene DgPAPS4 provided by the invention can promote flowering of plants with vernalization by regulating and controlling expression of vernalization pathway related genes, facilitates cultivation of plant varieties with vernalization in different ripening stages, reduces breeding workload, reduces breeding scale, shortens breeding age limit, improves breeding efficiency, and has a wide application prospect. And the breeding of plant varieties with vernalization is accelerated.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a gene affecting plant vernalization and flowering, a protein encoded by the gene, an expression vector and a host cell containing the gene, and a primer set for cloning the gene from plant DNA , also relates to the use of this gene. Background technique [0002] Dactylis glomerata L., also known as Orchardgrass (Orchardgrass), belongs to the Poaceae (Gramineae) Pooideae (Pooideae) Dactylis (Dactylis), is a perennial cool-season cluster widely cultivated worldwide grass. Duck grass has the characteristics of fast growth rate, high biological yield, high sugar content, strong shade tolerance and wide adaptability. As one of the top four perennial pastures by economic value, Duckweed is important for herbivore meat and dairy production in temperate regions of the world. In addition to being an excellent forage grass, Duckweed is also one of the important mixed-sow grasses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/02A01H6/46
CPCC07K14/415C12N15/827Y02A40/146
Inventor 张新全杨忠富黄琳凯聂刚冯光燕李丹丹汪霞周冀琼
Owner SICHUAN AGRI UNIV
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