Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

39results about How to "Help nurture" patented technology

Rapid propagation method for dysosma versipellis

The invention discloses a rapid propagation method for dysosma versipellis. The rapid propagation method includes the following steps that 1, sterile dysosma versipellis explants are manufactured; 2, the sterile explants are cultured through an induction medium to obtain embryonic callus; 3, the embryonic callus is cultured through a first multiplication medium, and the embryonic callus is subcultured for 2-3 generations to obtain subcultured embryonic callus; 4, the subcultured embryonic callus is cultured through a differentiation medium to obtain somatic embryos; 5, the somatic embryos are cultured through a second multiplication medium to obtain subcultured somatic embryos; 6, the subcultured somatic embryos are cultured through a plant regeneration medium to obtain dysosma versipellis plants. According to the rapid propagation method, the obtained dysosma versipellis embryogenic cell can induce regeneration of the dysosma versipellis plants, and the dysosma versipellis plants have the advantages of being high in growth rate and plant regeneration frequency, stable in character and the like.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Malus domestica 4-coumaric acid: coenzyme A ligase 4 gene and encoding protein and application thereof

The invention belongs to the field of genetic engineering, and particularly relates to a malus domestica 4-coumaric acid: coenzyme A ligase gene Md4CL4 and an encoding protein and application thereof.The gene is a 4CL gene Md4CL4 cloned from malus domestica. The gene is cloned from the malus domestica for the first time and provides genetic resources for plant molecular breeding. By utilizing a transgenic technology, an overexpression vector of the Md4CL4 gene is transferred into tobacco to obtain transgenic tobacco. The Md4CL4 gene is discovered from the malus domestica for the first time, due to high-response salt stress, a DNA fragment of a complete coding segment of the gene which is separated and cloned from the malus domestica and promotes salt stress tolerance is obtained, the functions of the gene are verified, and by utilizing the functions of the gene, it is finally discovered that the salt tolerance of a transgenic material obtained after the overexpression of the Md4CL4 gene in the tobacco is significantly improved. By means of the gene, the cultivation of new plant varieties with high salt tolerance is facilitated.
Owner:SHANDONG INST OF POMOLOGY

Molecular marker for wheat functional genes NAM-B1 and application of molecular marker

The invention relates to a molecular marker for wheat functional genes NAM-B1 and application of the molecular marker. Primer pair sequences for carrying out specific PCR (polymerase chain reaction) amplification on the molecular marker SpNAM-B1 for the wheat functional genes NAM-B1 are shown as SpNAM-B1-F and SpNAM-B1-R. The molecular marker and the application have the advantages that whether wheat contains the functional genes NAM-B1 or not can be identified by the aid of the molecular marker; the molecular marker can be used for carrying out high-protein molecular breeding on the wheat, the functional genes NAM-B1 for controlling grain protein contents can be transferred into receptor materials without the functional genes under assisted selection effects of the molecular markers, and accordingly the molecular marker and the application are beneficial to cultivating high-protein new wheat varieties with high iron and zinc contents.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Cotton casein kinase gene and its application

The invention discloses a cotton casein kinase gene and its application, and relates to a separation cloning, a function verification and an application of a gene GhCKI used for controlling anther growth and identified in a cotton anther expression spectrum. There is a 72% homology between the protein coded by the GhCKI gene and the protein coded by an Arabidopis thaliana ATCKL2 gene, and the GhCKI gene preferentially expresses in the anther, has an anther growth controlling function, can be applied to the sterile line cultivation of a plurality of plants through genetic transformation, and simultaneously can be used as a marker gene of the plant anther fertility stability under high temperature stress.
Owner:HUAZHONG AGRI UNIV

Seed germination treatment method of Chinese rhus succedanea

InactiveCN105993281AReduced seed processing timeLow costSeed and root treatmentSowingLacquer
The invention relates to a method for treating plant seeds, specifically a method for treating the germination of Chinese wild sumac seeds. The seeds are collected together with the peel, the seeds are exposed to the sun and separated from the peel, dried in the shade and stored until the spring of the second year, and soaked in carbendazim solution: then use Soak in warm water, wash the seeds with clean water, drain the seeds and sow. The Chinese wild sumac seed germination treatment method provided by the present invention uses physical stimulation as a means to effectively break the characteristics of hard, dense and waxy seeds of wild sumac seeds, which are not easy to germinate, and contribute to the cultivation of high-quality seedlings of wild sumac and germination The efficiency reaches more than 90%. Compared with the conventional seed treatment method, the seed treatment time is shortened by two-thirds, and the seeds can be processed in batches on a large scale. The cost is low, the method is simple, and it is easy to operate.
Owner:SICHUAN ACAD OF FORESTRY

Automatic soilless culture production line

The invention discloses an automatic soilless culture production line, and belongs to the technical field of soilless culture. The automatic soilless culture production line is characterized by comprising a conveyor belt group, supporting frames and a plurality of soilless culture devices, wherein, each soilless culture device comprises a plurality of culture grooves; vegetable fixing discs are arranged on the inner walls of the culture grooves; two sliding grooves are formed in the inner wall of each culture groove, first stepping motors are arranged in the sliding grooves, and the first stepping motors are fixedly connected with the vegetable fixing discs; supporting transverse plates are fixedly connected to the outer sides of the two supporting frames correspondingly, and strip-shaped grooves are formed in the two supporting transverse plates correspondingly; second stepping motors and third stepping motors are installed in the strip-shaped grooves, the second stepping motors and the third stepping motors are connected with electric telescopic rods, rotating motors are arranged at the ends of the electric telescopic rods, and the rotating motors are connected with rotating parts; and a transverse part is fixedly connected between every two rotating parts, a plurality of sliding motors are arranged in the transverse parts, and the sliding motors are connected with picking blades. The automatic soilless culture production line is mainly used for automatically planting and picking vegetables, and the working efficiency and the automation degree are effectively improved.
Owner:SOUTH CHINA AGRI UNIV

Gene GauRev2 capable of remarkably improving verticillium wilt resistance of cotton and application of gene GauRev2

The invention discloses a gene GauRev2 capable of remarkably improving verticillium wilt resistance of cotton and application of the gene GauRev2. The gene has a nucleotide sequence as shown in SEQ ID NO. 1. According to the invention, the expression of the GausREV2 gene in cotton is inhibited through a virus-mediated gene silencing technology, and after a verticillium wilt spore suspension is inoculated, the silencing plant is more susceptible to diseases. In arabidopsis thaliana, resistance to verticillium wilt can be remarkably improved through heterologous overexpression of the GausREV2. Discovery and utilization of the GausREV2 are beneficial to cultivation of cotton varieties resistant to verticillium wilt, and the problems in cotton production are solved. The molecular mechanism of the GausREV2 on verticillium wilt resistance is deeply researched, so that the GausREV2 has important theoretical significance for breeding cotton varieties with high verticillium wilt resistance by cotton breeders.
Owner:NANJING AGRICULTURAL UNIVERSITY

Pre-harvest management method for slowing down softening of muskmelon fruits

The invention belongs to the technical field of muskmelon management and particularly relates to a pre-harvest management method for slowing down softening of muskmelon fruits. The method comprises the following steps: treatment of muskmelon seeds, management in two weeks after flowering, management of a reticulation forming period and pre-harvest 48-hour management. Compared with the prior art, the method has the advantages that the root activity of seedlings can be improved, and the stem diameter of a plant can be effectively increased, so that the cultivation of strong seedlings is facilitated; the content of hydrogen peroxide of the plant can be favorably increased till a young fruit period of muskmelons after flowering, so that the disease resistance of the plant is improved, and fungus infection to the plant in the period can be avoided; in the reticulation forming period, the deposition of lignin of the plant can be promoted, so that the permeation and extension of diseases can be prevented; the whole plant is sprayed with jasmonic acid methyl ester solution in 48h before harvest, so that the muskmelon fruits have a certain firmness, and the fruit firmness and the content of protopectin when the muskmelons are harvested can be effectively improved, therefore, the purpose of slowing down the softening of the muskmelon fruits is achieved.
Owner:界首市海棠湾生态农业科技有限公司

A kind of artificial cultivation method of dominant symbiotic mycorrhizal fungus of Chestnut

The invention relates to an artificial cultivating method for advantageous symbiotic mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils. The method comprises the following steps: (1) taking a fruiting body of mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils., and collecting fungus context issue blocks at the intersecting part of a fungus cap and a fungus stipe; (2) putting the fungus context tissue blocks in a culture plate, culturing the fungus context tissue blocks at the temperature of 25-28 DEG C until mycelia are germinated by the fungus context issue blocks, transferring the mycelia, and then cultivating the mycelia at the temperature of 25-28 DEG C until the mycelia grow to cover the culture plate to obtain purified mycelia; (3) selecting the purified mycelia, and inoculating the selected purified mycelia to an improved MMN liquid culture medium for fermental cultivation, thus obtaining a bacterium solution; (4) inoculating the bacterium solution to an enlarged cultivating material, and cultivating the purified mycelia at the temperature of 25-28 DEG C until the purified mycelia grow all over a cultivating container to obtain mycorrhizal fungi inoculant of Castanea henryi (Skam) Rehd. et Wils. The artificial cultivating method for advantageous symbiotic mycorrhizal fungi of Castanea henryi (Skam) Rehd. et Wils. is simple in operation; when mycorrhizal fungi cultivated and obtained through the artificial cultivating method is inoculated to seedlings of Castanea henryi Rehd. et Wils., the impregnating rate is high.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

A kind of processing method of lacquer tree seedling cultivation seed

InactiveCN103947329BHelp nurtureShort germination periodSeed and root treatmentWaxWarm water
The invention provides a processing method of sumac seedling seeds. The method comprises the steps of storing sumac seeds in sand, soaking the seeds in saline solution, and cleaning; soaking the seeds in washing powder water, rubbing the surfaces of the seeds to remove wax, and putting the seeds into a woven bag; spraying warm water onto the seeds every other 2 hours until the seeds are expanded and the seed coats are softened; putting the seeds into a breathable bamboo frame or a plastic frame, and watering until the seeds crack and sprout. According to the processing method, the characteristics that the sumac seedling seeds have after-ripening embryo, and seed shells are firm, seed coats are compact and the waxiness is thick can be effectively broken, the cultivation of the high-quality sumac nursery stock can be facilitated, the germination rate of the sumac seeds reaches more than 50% after 5-6 days, the sumac seeds can be planted in the nursery garden land by broadcast sowing or hill-seeding in the seventh day, the germination rate reaches up to more than 95% within 15 days after sowing, and 60% of seeds can germinate within a week. According to the processing method, the germination period is short, more than half time can be shortened, the seeds can be processed in batches on a large scale, and the cost is low; furthermore, a chemical reagent with high harmfulness is eliminated, and the processing method has the characteristics of being safe, environmentally friendly and easy to operate.
Owner:云南盛禾生态农业科技发展有限公司

Vegetable high-temperature stress regulation and control mixture and application method thereof

InactiveCN110981593AHigh activityEnhance active oxygen scavenging abilityBiocidePlant growth regulatorsPhosphateAlcohol sugars
The invention provides a vegetable high-temperature stress regulation and control mixture which comprises the following raw material components in percentage by weight: 0.05-0.15% of salicylic acid, 0.02%-0.05% of brassinolide; 0.3%-0.5% of urea; 0.05%-0.1% of gamma-aminobutyric acid; 0.3%-0.5% of glucose; 0.02% to 0.08% of mepiquat chloride; 0.1%-0.5% of potassium dihydrogen phosphate; 0.07%-0.1%of sugar alcohol boron; 0.07%-0.1% of sugar alcohol iron; 0.08%-0.1% of sugar alcohol calcium; 0.1%-0.3% of zinc sulfate heptahydrate (ZnSO<4>*7H<2>O); 0.1% of an agricultural organic silicon spreader; and 97.42%-98.74% of water. When the vegetable high-temperature stress regulation and control mixture prepared by the invention is adopted, the protective enzyme activity can be obviously improved;the content of malondialdehyde (MDA) is reduced to 1.62-1.67 [mu] mol.g <-1 > FW, in addition, the photosynthetic performance of leaves can be improved, the diseases such as element absorption obstacle, high-temperature nutrient deficiency of plants, root aging of vegetables and root activity reduction can be prevented and treated, the malformed fruit rate is reduced, and the effects of increasing the yield and improving the quality of over-summer vegetables are achieved.
Owner:WEIFANG UNIV OF SCI & TECH

BHLH gene influencing salt tolerance of populus sutchuenensis and application of bHLH gene

ActiveCN114231542AHelp nurtureExcellent resistance geneMicrobiological testing/measurementPlant peptidesBhlh genesMolecular biology
The invention discloses a bHLH gene influencing the salt tolerance of populus sutchuenensis and application thereof.The sequence of the bHLH gene is shown in a sequence table 1, the amino acid sequence of the bHLH gene is shown in a sequence table 2, and the application of the bHLH gene is that knockout expression of the bHLH gene influences the salt tolerance of populus sutchuenensis, so that breeding of salt-tolerant poplar new germplasm is facilitated; research results provide excellent resistance genes for improvement of salt-tolerant varieties of poplars, and have important scientific significance.
Owner:SHENYANG AGRI UNIV

Substrate for raising seedlings from seeds of Rhizoma Areactylodis Lanceae and preparation method and application of substrate

The invention discloses a substrate for raising seedlings from seeds of Rhizoma Areactylodis Lanceae and a preparation method and application of the substrate. The substrate includes peat, vermiculite, perlite and rotten chicken manure at a preferable ratio of 3:1:1, and the amount of the rotten chicken manure is 5-8 kg/m<3>. Strong seedlings can be raised when the substrate is used for raising seedlings from seeds of Rhizoma Areactylodis Lanceae, and a fertilization and management method which is matched with the substrate is simple, and has easy implementation; after optimization is carriedout, and the method which meets the suitable formula ratio of the seedling substrate of Rhizoma Areactylodis Lanceae and the fertilization ratio and management in the seedling period is proposed through comprehensive evaluation of seedling fresh weight, the chlorophyll content, root vitality and other indicators; and the produced seedlings are robust, the quality is uniform, the weight of the seedlings is 2 times the weight of even-aged Rhizoma Areactylodis Lanceae seedlings which are obtained through open culture, and the method is of great significance to cultivation of high-quality RhizomaAreactylodis Lanceae.
Owner:甘肃广药白云山中药科技有限公司 +2
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products