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BHLH gene influencing salt tolerance of populus sutchuenensis and application of bHLH gene

A technology of Salt Tolerance of Populus japonicus, which is applied to the bHLH gene that affects the salt tolerance of Populus japonicus and its application field, and can solve problems affecting plant growth and development

Active Publication Date: 2022-03-25
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Salt stress is the main environmental stress affecting plant growth and development, but there is no report on the salt tolerance function of the bHLH transcription factor family genes of Populus japonicus

Method used

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  • BHLH gene influencing salt tolerance of populus sutchuenensis and application of bHLH gene
  • BHLH gene influencing salt tolerance of populus sutchuenensis and application of bHLH gene
  • BHLH gene influencing salt tolerance of populus sutchuenensis and application of bHLH gene

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Experimental program
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Effect test

Embodiment

[0171] 1 Bioinformatics Analysis of bHLH Transcription Factor Family Genes in Populus montana

[0172] 1.1 Cluster analysis of the bHLH genes of Populus montana and Arabidopsis

[0173] The amino acid sequences of 113 bHLH genes were obtained from the Populus montana genome database, and the phylogenetic tree was constructed with 9 Arabidopsis thaliana bHLH genes known to be related to abiotic stress, and the results were as follows Picture 1-1 As shown, it can be seen from the figure that the bHLH1 gene marked by the red symbol is in the same evolutionary branch as the Arabidopsis salt-tolerance-related gene (AT4G02590), indicating that this gene may be involved in the salt stress response of Populus japonicus.

[0174] 1.2 Multiple sequence analysis of the bHLH1 gene of Populus japonicus and the bHLH gene of Arabidopsis thaliana

[0175] The amino acid sequences of the screened Populus japonicus bHLH1 gene and the Arabidopsis thaliana bHLH gene (AT4G02590) were analyzed by ...

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Abstract

The invention discloses a bHLH gene influencing the salt tolerance of populus sutchuenensis and application thereof.The sequence of the bHLH gene is shown in a sequence table 1, the amino acid sequence of the bHLH gene is shown in a sequence table 2, and the application of the bHLH gene is that knockout expression of the bHLH gene influences the salt tolerance of populus sutchuenensis, so that breeding of salt-tolerant poplar new germplasm is facilitated; research results provide excellent resistance genes for improvement of salt-tolerant varieties of poplars, and have important scientific significance.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a bHLH gene affecting the salt tolerance of Populus japonicus and its application. Background technique [0002] Transcription factors are master regulators of stress-responsive genes and are preferred for plant improvement. However, many transcription factors in different plants remain to be discovered, and among more than 80 families, only a few transcription factors have been studied in depth to have roles in biotic and abiotic stress responses. Basic helix-loop-helix proteins (bHLH) are the most widespread class of transcription factors in eukaryotes, regulating gene expression by recognizing and interacting with specific motifs in target genes; not only universally involved in plant growth and Metabolism, and also play an important role in plant responses to stress. However, most studies on bHLH transcription factors have focused on a small number o...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/00C12Q1/6895
CPCC07K14/415C12N15/8273C12Q1/6895C12Q2600/158C12Q2600/13
Inventor 及晓宇李磊国会艳王玉成李丹丹
Owner SHENYANG AGRI UNIV
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