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Device and method for rapidly separating monospora strains of rice blast germs

A technology of rice blast bacteria and strains, which is applied in the field of microbiology technology and plant protection, can solve the problems of restricting progress and level, easy-to-contaminate strains, long separation and purification cycles, etc., and achieve the effect of avoiding pollution and shortening the time

Pending Publication Date: 2020-06-19
XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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  • Description
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Problems solved by technology

At present, the existing methods for the isolation of single spores from Magnaporthe grisea have cumbersome separation processes, high separation costs, long separation and purification cycles, easy contamination during the separation process, and the isolated strains cannot be single-spore strains.
These factors have greatly restricted the progress and level of researchers in studying the spatio-temporal dynamics of avirulent genotypes in Magnaporthe grisea populations

Method used

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  • Device and method for rapidly separating monospora strains of rice blast germs
  • Device and method for rapidly separating monospora strains of rice blast germs
  • Device and method for rapidly separating monospora strains of rice blast germs

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Embodiment Construction

[0040] In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

[0041] Second, "one embodiment" or "an embodiment" referred to herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. "In one embodiment" appearing in different places in this specification does not all refer to the same embodiment, nor is it a separate or selective embodiment that is mutually exclusive with other embodiments.

[0042] Secondly, in the following descriptions, all concepts involving directionality (or orientation) such as up, down, left, right, front...

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Abstract

The invention provides a device for rapidly separating monospora strains of rice blast germs. The device comprises an operation platform, a driving tank, a teasing needle and a strain bearing platform, wherein a light hole is formed in the operation platform, a driving system is arranged in the driving tank, the strain bearing platform is arranged along the circumferential direction of the light hole and is used for bearing a to-be-operated rice straw, the teasing needle is driven by the driving system to move along the horizontal and vertical directions, the tip of the teasing needle is in contact with the rice straw.

Description

technical field [0001] The invention relates to a microbiology technology and a plant protection technology, in particular to a device and a method for rapidly isolating monospore strains of the rice blast fungus. Background technique [0002] Rice blast is a worldwide disease caused by ascomycete fungi, which can cause a substantial reduction in rice production, in severe cases, by 40% to 50%, or even failure. Rice blast can occur throughout the growth period of rice, and has the characteristics of long damage time, diverse symptoms and multiple infection sites. Due to the genetic complexity of the rice blast fungus race and the rapid mutation of the non-toxic gene, the resistance of resistant varieties will be weakened or lost after 3 to 5 years of popularization and planting. Real-time and accurate understanding and grasp of the population composition of blast fungus and the dynamic trend of avirulent gene changes in physiological races in each rice area can provide a ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N1/14C12N1/02C12R1/645
CPCC12M33/04C12N1/02C12N1/14
Inventor 何弯弯王健康丁成伟郭荣良吴玉玲徐家安王友霜胡婷婷赵轶鹏
Owner XUZHOU INST OF AGRI SCI IN JIANGSU XUHUAI DISTRICT (JIANGSU XUZHOU SWEETPOTATO CENT)
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