Intragenic single nucleotide polymorphism (SNP) mark of male sterility restoring gene RF4 of C-type cytoplasm of corn

Abstract

The invention discloses an intragenic single nucleotide polymorphism (SNP) mark of a male sterility restoring gene RF4 of C-type cytoplasm of corn. The intragenic SNP mark is obtained by the following steps of: constructing a near-isogenic line by using a good inbred line 87-1 of corn as a basic material, and performing DNA (deoxyribonucleic acid) extraction on leaves of a single plant of an offspring of the near-isogenic line; performing molecular marker development on the extracted DNA, and acquiring and processing molecular marker data; screening an 87-1 BAC library by using primers on two sides of a target gene to obtain a clone containing the restoring gene RF4, sequencing the clone, and performing gene prediction according to a target sequencing result; and performing functional analysis according to the result of gene prediction, and obtaining the intragenic SNP mark of the male sterility restoring gene of the C-type cytoplasm. According to the invention, fine positioning of the male sterility restoring gene of the C-type cytoplasm and the intragenic SNP mark of a fertility restoring gene are realized, and the technical problem in molecular detection of the male sterility restoring gene of the C-type cytoplasm of corn is solved.

Description

technical field [0001] The invention belongs to the technical field of gene inheritance, and in particular relates to an intragenic SNP marker of a maize C-type cytoplasmic male sterility restorer gene RF4 and an application thereof. Background technique [0002] Male sterility is common in higher plants, and male sterility can be divided into nuclear sterility and cytoplasmic sterility. Among them, cytoplasmic male sterility is caused by cytoplasmic sterility gene (S) and nuclear sterility gene ( rfrf) interaction controls the type of sterility. One of the cytoplasmic genes and nuclear genes has a normal recovery gene, and the fertility can be restored. The genotype of the sterile line is S (rfrf); the genotype of the maintainer line is N (rfrf), which is used as the male parent to cross the sterile line, and the offspring obtained can maintain the sterility of the female parent; the gene of the restorer line The types are S(RfRf) and N(RfRf). When it is used as the male ...

AI Technical Summary

Problems solved by technology

[0005] The present invention provides an intragenic SNP marker of the corn C-type cytoplasmic male sterility restorer gene RF4 and its application, aiming to solve the problem of the failure of the prior art system to realize the fine positioning of the corn C-type cytoplasmic male sterility restorer gene and the Problems with intragenic labeling based on DNA sequencing

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