The invention provides a novel test paper strip as well as preparation and application of the novel test paper strip. The preparation comprises the following steps: A, preparing a base plate with the corresponding size; B, coating the base plate with a test paper plate which is matched with the base plate and contains reaction chemicals; C, printing bar codes on the base plate, wherein information of the bar codes comprises the modes of the test paper strips, true and false information, and the cis-trans states of the strips; and judging the cis-trans states by scanning the bar codes by a phone App; D, coloring the base plate, wherein the colors are colors with the standard values; E, cutting the test paper plate along small square blocks coated with glue, wherein only the test paper plate is cut and the base plate is not cut; and rotating and blowing off the test paper plate which is not coated with the glue by wind; and F, cutting the base plate along lines among the bar codes so as to obtain the test paper strip. By adopting the scheme, the accuracy that an intelligent phone directly scan and read color data can be improved greatly, so that the practicability of phone applications can meet the detection requirements.