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69results about How to "Promote late growth" patented technology

Tobacco package fertilization method

ActiveCN102598947ASimplify labor proceduresRational balance of tobacco fertilizationFertilising methodsFertilizer mixturesNutrientFertilizer
The invention relates to a tobacco package fertilization method, which is mainly characterized in that three kinds of fertilizers: special tobacco active biological organic and inorganic fertilizers, special tobacco seedling extracting fertilizers and special tobacco additional fertilizers are adopted, and the fertilizers with different nutrition mixture ratios are adopted according to different growth periods of the tobacco for promoting the growth of the tobacco. The tobacco package fertilization method is guided by a tobacco fertilization demanding rule, different forms of fertilizers withdifferent nutrients are applied in different field growth periods of the tobacco for promoting the tobacco to realize the better growth, and the fertilization method has the effects of simplifying the tobacco farmer work procedure, reasonably balancing the tobacco fertilization, improving the tobacco use value and the like, so the goals of reducing the work intensity of tobacco farmers, enhancingthe efficiency, improving the quality of the tobacco leaves and increasing the yield are reached.
Owner:湖南金叶众望科技股份有限公司

In-bottle production method of detoxified small seed ball of east lily

The invention provides a process for producing the oriental lily sterilized seed globule in the bottle, comprising: choosing explant, sterilizing, inducing culture and getting the sterilized stem tip, then breeding culture, subculture, and producing the sterilized seed globule in the bottle directly. The invention produces the sterilized seed globule in the bottle directly to avoid the problem of long period of ball forming and the accumulation of virus existing in the oriental lily, the seed globule can be planted in the soil when out of the bottle, and the survival rate is high. The breeding procedure of the sterilized seed globule is simplified by modifying and optimizing the whole producing procedure for the lily seed globule, and the breeding time of sterilizing the oriental lily is shortened greatly, the quality of seed globule is improved when the producing cost is decreased, which reinforces market competitive power of the domestic self-breeding seed globule and is suitable for the mass production of the oriental lily sterilized seed globule.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Handheld onion transplanting device

InactiveCN103283364AStandard planting depthPromote late growthTransplantingHand heldEngineering
The invention relates to a handheld onion transplanting device. The device comprises a hole punching mechanism, a seedling guide mechanism and an earthing mechanism. The hole punching mechanism is used for penetrating through a mulching film to punch a taper hole and is a stander of the whole device; a seedling guide pipe in the seedling guide mechanism downwards moves to unfold a taper hole punching machine arranged at the lower end, normative field planting holes are punched in the earth, and seedlings enter the field planting holes under the action of the seedling guide pipe; the earthing mechanism is used for earthing after the seedlings are guided, so that the vertical state after the seedlings are transported can be guaranteed, and the transportation can be completed. The handheld onion transplanting device is simple in structure and convenient to operate, the work efficiency can be improved, and the labor intensity can be reduced. The uniformity of the transplanting depth can be realized, the device is good for growth of the seedlings at the later period, and the production and the income of onions can be preferably increased.
Owner:SHANDONG AGRICULTURAL UNIVERSITY

Tea-oil tree seedling culture light matrix and preparing method and application thereof

The invention discloses a tea-oil tree seedling culture light matrix and a preparing method and application thereof. The tea-oil tree seedling culture light matrix is mainly prepared by evenly mixing saw dust, litter, biogas residues and yellow subsoil, and is prepared from, by weight, 15-24 parts of saw dust, 45-65 parts of litter, 3-6 parts of biogas residues, 8-10 parts of chicken manure, 0.007-0.015 part of microbial agents, 1-2 parts of urea, 3-5 parts of superphosphate and 12-18 parts of yellow subsoil. The tea-oil tree seedling culture light matrix is simple and reasonable in formulation, comprehensive in nutrition and stable in quality, raw materials are sufficient, waste resources can be fully used, environment pollution is reduced, the tea-oil tree seedling culture cost is reduced, the utilization path of resides and waste organic matter in a forest area is expanded, and high-quality and efficient natural organic fertilizer is fully used. The survival rate of tea-oil tree seedlings ranges from 98% to 100%, the average increasing amount of the heights of the seedlings reaches 6.80-7.07 cm, and the average increasing amount of the ground diameter reaches 0.18-0.21 mm; good economical benefits, social benefits and ecological benefits are achieved.
Owner:GUANGXI FORESTRY RES INST

Method for separating and culturing mouse synovial cells

The invention provides a method for isolating and cultivating mouse cells, which includes the following steps: (1) sterilizing tissue processing equipment; (2) selecting male BALB / c mice aged 1 to 2 months, killing them by breaking their necks, and using surgical scissors. Separate the synovial layer and fibrous layer of the joint capsule, then remove the synovial layer tissue, put the tissue into pre-cooled sterile PBS solution containing double antibody and wash it several times to remove blood stains and fatty tissue; (3) Cut the tissue into pieces, and use Digest with preheated type II collagenase and trypsin respectively; (4) Stop digestion, centrifuge, and discard the supernatant; (5) Inoculate into the coated culture flask by manual adhesion method; (6) After incubation for 1 to 2 hours, Cultivate in a 37°C, 5% CO2 environment; (7) Cell morphology observation; (8) Cell subculture; (9) Cell identification. The method for isolating and culturing mouse synovial cells provided by the invention is simple to operate, has a large number of cells obtained, and has a high survival rate. It is an ideal primary method for isolating and cultivating mouse synovial cells and provides reliable results for experiments. Cell resources.
Owner:JIANGYIN CHI SCI

Organic rice cultivation method by utilizing farmland and rice chaff weeding

The invention discloses an organic rice cultivation method by utilizing farmland and rice chaff weeding, belonging to the technical field of rice production. The method comprises the steps of: soaking in shallow water; ploughing for two times or three times; transplanting strong seedlings; broadcasting rice chaff; conducting later field management and other steps. In the method, drainage is required at first and then ploughing is carried out each time, which aims at carrying away sheathed monochoria herb without spouting and floating on the surface of a water level and seeds of chufa through drainage; the rice chaff fertilization faster improve the field temperature so as to create a good sprouting environment for weed seeds, and also can reduce the oxygen content under the surface layer, thus preventing the normal growth of the weed roots and achieving purpose of killing weeds, further increasing the content of organic matters, being beneficial to improvement of rice quality. The organic rice cultivation method can be popularized and applied in rice cultivation regions.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Separating and culturing method for olfactory ensheathing cells of rats

The invention provides a separating and culturing method for olfactory ensheathing cells of rats. The separating and culturing method comprises the following steps: (1) disinfecting a tissue processing instrument; (2) selecting male SD rats of 2-3 months old, killing the male SD rats after anesthesia, separating the olfactory mucosa tissue by adopting surgical scissors, placing the tissue in PBSsolution which is precooled, is sterile and contains double antibodies, and washing the tissue for a plurality of times, so that blood stains and outer membranes are removed; (3) cutting up the tissue, and carrying out digestion by adopting preheated neutral enzyme and trypsin respectively; (4) terminating the digestion, carrying out centrifugation, and discarding the supernatant; (5) inoculatingthe obtained product into an enveloped culture flask by adopting an artificial wall sticking method; (6) carrying out incubation for 1-2 h, and carrying out culturing in the environment being at 37 DEG C containing 5% CO2; (7) carrying out cell purification; (8) carrying out cell morphological observation; (9) carrying out cell subculturing; (10) carrying out cell counting and survival rate testing; and (11) carrying out immunological identification. For the separating and culturing method for olfactory ensheathing cells of rats, the operation is simple, a large number of cells are obtained, the survival rate is high, the method belong to an ideal primary separating and culturing method for the olfactory ensheathing cells of rats, and a reliable cell resource is provided for the experiment.
Owner:JIANGYIN CHI SCI

Water and fertilizer conservation on mine side slope planting tree bag solid pit planting method

The invention relates to the technical field of restoration of the ecological environment in mining areas, in particular to a planting method for water and fertilizer conservation on mine slopes, planting tree bags and solid pits, which is suitable for slopes with small slopes. Drill the nail pile under the tether, take soil samples and test, determine the amount of water-retaining agent and rooting powder according to the test results, fully mix the water-retaining agent with the soil, organize digging, organize pipeline laying, and tree planting, use 0.4~0.8×0.3~0.8 m Planting bags for water and fertilizer conservation, setting up soil retaining frames, daily maintenance, focusing on organizing watering, fertilization and weeding according to the growth habits of seedlings, and strengthening the prevention and control of diseases and insect pests. The invention can promote the development and utilization of mineral resources, stepping into a virtuous circle with good economic benefits, low resource consumption and less environmental pollution. It provides a set of fast, economical and practical methods for the ecological restoration of mining areas in the future and the ecological restoration of mining areas in similar areas at home and abroad.
Owner:ANSTEEL GRP MINING CO LTD

Feed for weaned piglets and feeding method thereof

The invention discloses a feed for weaned piglets. The feed is composed of Zhubao rice flour, a creep feed and a piglet feed. The Zhubao rice flour and the creep feed are used for breeding a piglet both three days before weaning and three days after weaning; then the piglet feed gradually replaces the creep feed for feeding of the piglet within 7 days; and then about 30 kg of the piglet feed is used for feeding the piglet for 30 to 35 d, and the body weight of the piglet is increased to 30 kg. According to the invention, based on the characteristics of the growth of piglets in different growthstages, the feed conforming to the growth characteristics of piglets is provided on the premise of reducing the usage amount of antibiotics; the feed digestion and absorption rates of the piglets areincreased; the stress syndrome of the piglets is reduced; the diarrhoea rate of the piglets is lowed; the survival rate and growth speed of the piglets are improved; and the weight uniformity of a pig group is enhanced.
Owner:中粮饲料(茂名)有限公司

Wheat equal-depth broadcast sowing and under-seed equal-depth fertilizer spreading method

The invention provides a wheat equal-depth broadcast sowing and under-seed equal-depth fertilizer spreading method, and relates to the technical field of wheat cultivation and fertilization. The method comprises the following steps: A, performing rotary tillage; B, performing equal-depth fertilizer spreading, spreading fertilizer by using an equal-depth fertilizer spreading device, partially falling turned soil under the fertilizer, partially covering the fertilizer, and spreading the fertilizer to the middle lower part of a rotary tillage layer; C, performing broadcast sowing of wheat, performing broadcast sowing of wheat seeds by using an equal-depth broadcast sowing device, falling the turned soil on the seeds to cover the seeds with the soil, and applying the seeds on the same depth on the middle upper part of the rotary tillage layer; D, finally suppressing. The defects of wheat drilling and broadcast sowing are overcome, the advantages of the drilling and the broadcast sowing are gathered, the utilization rate of the fertilizer and the moisture utilization rate are greatly improved, and the wheat yield per unit area is improved; meanwhile fertilizer input and underground water exploitation are reduced, the crop growth environment is favorably improved and the agricultural ecological environment is favorably protected.
Owner:CHINA AGRI UNIV

Formula and preparing method of base fertilizer and leaf fertilizer for increasing apple production

The invention relates to a formula of base fertilizer and leaf fertilizer for increasing apple production. According to the formula, thoroughly decomposed waste bacteria sticks, thoroughly decomposed dung, thoroughly decomposed Chinese medicine residues, fine sand and microelement fertilizer are mixed according to a certain metering standard to obtain the base fertilizer, and biogas slurry and amino acid fertilizer are diluted with water to obtain the leaf fertilizer. According to the formula of the base fertilizer and leaf fertilizer for increasing the apple production, the base fertilizer is applied according to a standard of 250-300kg each tree in autumn, and the leaf fertilizer is applied to the crown of each apple tree in April of the next year; after the application of the base fertilizer and the leaf fertilizer, the waste bacteria sticks, nutrient substances such as organic matter contained in the dung and the Chinese medicine residues can promote the growing of roots and healing of broken roots, microelement can promote the later-period growing of apple trees, and meanwhile, various components are matched with one another to improve the soil property and lay a firm foundation for the high yield of the apple trees in the next year.
Owner:TIANJIN HONGTONG FRUIT CULTIVATION PROFESSIONAL COOP

Degradable irrigation-free haloxylon ammodendron protective device

The invention relates to the technical field of irrigation-free haloxylon ammodendron protective pipe fittings, and discloses a degradable irrigation-free haloxylon ammodendron protective device. The degradable irrigation-free haloxylon ammodendron protective device comprises supporting straw and a protective layer, wherein the left part of the protective layer and the right part of the protective layer are fixedly mounted together; a protective cavity is formed inside the protective layer; two or more pieces of supporting straw are fixedly mounted on the protective layer at intervals in the longitudinal direction, and are distributed around the protective cavity. According to the degradable irrigation-free haloxylon ammodendron protective device disclosed by the invention, the seedling culturing, the transplanting and the protecting of haloxylon ammodendron can be realized; meanwhile, the degradable irrigation-free haloxylon ammodendron protective device disclosed by the invention can prevent haloxylon ammodendron seedlings from being burnt by a high temperature and ensure the normal growth of the haloxylon ammodendron, so that the survival rate of the haloxylon ammodendron is improved; the recovery speed and reconstruction speed of the haloxylon ammodendron woodland in a desert and semi-desert environment are accelerated; the degradable irrigation-free haloxylon ammodendron protective device has a very good industrial application prospect; the popularization and the application of an irrigation-free haloxylon ammodendron protective technology are promoted.
Owner:XINJIANG AGRI UNIV

Furrowing profiling pneumatic type precision vegetable seeder

ActiveCN105874944AEnsure consistencyEffective soil looseningSpadesPlantingSeederAgricultural engineering
The invention discloses a furrowing profiling pneumatic type precision vegetable seeder. The precision vegetable seeder comprises a main frame and a profiling unit. A three-point suspension rack is fixed at the front end of the main frame. A seeding unit is fixed at the rear end of the main frame. A pneumatic unit is fixed above the main frame. A furrowing shaping unit is fixed below the main frame. The profiling unit is mounted on the main frame. The furrowing profiling pneumatic type precision vegetable seeder has the advantages that operations such as furrowing, compacting and seeding can be completed in one step, and the seeder is high in operation efficiency and high in adaptability.
Owner:HUAZHONG AGRI UNIV

A winter wheat high yield planting technology

The invention relates to a winter wheat high yield planting technology comprising seed treatment, soil preparation treatment, seeding and field management. In the technology, firstly a compound seed dressing agent is used for seed dressing, wherein drug ingredients can effectively prevent underground insect attack, full rot, powdery mildew and the like, and other ingredients can improve the lodging resistance and disease resistance, promote root development and tillering and achieve an effect of product increase; during soil preparation, fertilizer and field soil are fully mixed to facilitate seedling emergence and later growth; through experiments, the using amount per mu in seeding is made to be the most appropriate using amount, so that waste is prevented and the yield per mu is guaranteed; the later field management is performed according to the growth conditions of wheat in different stages, so that the yield per mu and the quality of wheat can be further increased.
Owner:TIANJIN JI COUNTY BAIYUN HEITU CROP GROWING SPECIALIZED COOP

Method for artificially inoculating pine wood nematodes by semiannual masson pine seedling tender tip skin scratch and application thereof

The invention discloses a method for artificially inoculating pine wood nematodes by a semiannual masson pine seedling tender tip skin scratch and an application thereof. The method comprises the following steps of: (1) preparing living pine wood nematodes into a pine wood nematode suspension; (2) selecting a tender tip position 3-5cm below the tip of a semiannual masson pine seedling, pulling outneedle leaves, and longitudinally scratching two or more wounds at the skin of the tender tip of the masson pine from which the needle leaves are pulled out; (3) enclosing a small water-tight funnelat the part where the needle leaves are removed by using a sealing film; wherein the lower part of the small funnel clings to the tender tip of the masson pine and is flush with the lower part of thewounds; (4) injecting the pine wood nematode suspension into the small funnel to inoculate the pine wood nematodes; and (5) placing the semiannual masson pine seedlings inoculated with the pine wood nematodes in an outdoor environment for cultivation. The method is suitable for artificially inoculating pine wood nematodes to the semiannual masson pine seedlings, can effectively reduce production cost, and has wide application prospect and important production significance.
Owner:SOUTH CHINA AGRI UNIV

Novel corn zero-tillage fertilization application and sowing machine with function of triangular final seedling singling

PendingCN110506462AIncrease seeding densityAchieve dense plantingSpadesPlantingAgricultural engineeringSeedling
The invention discloses a novel corn zero-tillage fertilization application and sowing machine with the function of triangular final seedling singling. A hoeing and soil loosening shovel and a depth wheel on an existing corn sowing machine are removed, a seed belt processing device is installed at the position of the hoeing and soil loosening shovel, and multiple twining-prevention cutter blade sets capable of following a cutter shaft to rotate are arranged on the cutter shaft of the seed belt processing device, each twining-prevention cutter blade set corresponds to a sowing opener set at therear position, a land wheel rotates and drives seeding devices and fertilizing devices to rotate, and through the sowing opener sets and fertilizer application openers, fertilization application andsowing work is conducted in a processing area formed through rotation of the cutter shaft of the seed belt processing device. According to the novel corn zero-tillage fertilization application and sowing machine with the function of triangular final seedling singling, the seed belt processing device is adopted for replacing the hoeing and soil loosening shovel and the depth wheel on the existing corn sowing machine, then straw and soil are smashed, the spacing of the corresponding seeding devices is adjusted, positioned sowing on seeds is achieved, the triangular final seedling singling sowingfunction is achieved, and the sowing density of corn is increased on the condition that the sowing plant spacing and the basic row spacing are not changed.
Owner:漯河市农机推广服务中心

Biological concentrate supplement for breeding fat cattle and sheep

The invention discloses a biological concentrate supplement for breeding fat cattle and sheep. The biological concentrate supplement for breeding fat cattle and sheep is prepared from the following active ingredients in parts by weight: 10-15 parts of astragalus membranaceus, 10-15 parts of herba epimedii, 4-8 parts of semen cuscutae, 4-8 parts of sweet wormwood, 4-8 parts of folium isatidis, 10-15 parts of hawthorn fruits, 1-3 parts of procyanidin and 1-3 parts of lactobacillus sporogenes. The biological concentrate supplement for breeding fat cattle and sheep is capable of resisting bacteria and diminishing inflammation, tonifying spleen and promoting appetite, enhancing the disease resisting ability and the anti-stress ability and enhancing the immunity of cattle and sheep, contains no antibiotics, and is capable of increasing the feed conversion ratio, reducing the breeding cost, shortening the production period, increasing the dressing percentage, improving the flavor of meat and improving the breeding efficiency.
Owner:杨永森

A kind of sticking and grafting technology of double-shaved surface of Chinese torreya

InactiveCN103766143BHigh wedgingTake full advantage of growth potentialHorticultureRootstockEngineering
The invention discloses a double-cut surface high-low level joint grafting technology for Torreya grandis. The joint grafting technology is a new grafting method that the long cut surface of a stock is inwards cut bias from bottom to top to the truncation surface, and moreover, the root of the long cut surface of the stock is vertically cut downwards along the part between bark and xylem to form the short cut of the stock; the long cut surface of a scion and the long cut surface of the stock are grafted in a joint manner; the short cut surface of the scion is inserted into the short cut of the stock, and thus, not only is the conjunction degree of the scion and the stock high, are the scion and the stock high more tightly and is the healing power stronger but also the scion is basically vertical upwards, and crown inclination cannot occur after the scion survives; only branches, a terminal bud and 4-5 leaves of the scion are kept, so that the transpiration of the blades is reduced, and the moisture loss is reduced. According to the joint grafting technology disclosed by the invention, only the terminal bud, which is about 1cm, of the scion is kept, and thus, the distance from the stock to the terminal bud is shortened, the sprouting capability of the terminal bud is enhanced, and the grafting survival rate reaches up to 98 percent. A Torreya grandis grafted plant is upright; the crown does not incline; the grouting capability is strong; the crown can be rapidly formed.
Owner:胡冬益 +2

Human primary gastric cancer cell separation and culture method

The invention provides a human primary gastric cancer cell separation and culture method. The method comprises the following steps of (1) disinfecting a tissue processing device; (2) using a pair of operating scissors for separating a gastric cancer tissue, placing the tissue into a precooled sterile PBS liquid containing double antibodies, and washing for multiple times to remove bloodstains andnecrotic tissues; (3) cutting up the tissues, and using preheated IV type collagenase and trypsin for digesting respectively; (4) terminating digestion, centrifuging, and removing a supernatant; (5) after carrying out cell counting, inoculating into a coated culture flask; (6) culturing at 37 DEG C in an environment with 5 percent of CO2; (7) purifying the cells; (8) subculturing the tissues; (9)observing cell morphology; (10) determining cell survival rate; (11) carrying out immunological identification. The invention provides the human primary gastric cancer cell separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human gastric cancer cell primary separation and culture method, and a reliable cell resource is provided for experiments.
Owner:JIANGYIN CHI SCI

Double-cut surface high-low level joint grafting technology for Torreya grandis

InactiveCN103766143AHigh wedgingTake full advantage of growth potentialHorticultureEngineeringBud
The invention discloses a double-cut surface high-low level joint grafting technology for Torreya grandis. The joint grafting technology is a new grafting method that the long cut surface of a stock is inwards cut bias from bottom to top to the truncation surface, and moreover, the root of the long cut surface of the stock is vertically cut downwards along the part between bark and xylem to form the short cut of the stock; the long cut surface of a scion and the long cut surface of the stock are grafted in a joint manner; the short cut surface of the scion is inserted into the short cut of the stock, and thus, not only is the conjunction degree of the scion and the stock high, are the scion and the stock high more tightly and is the healing power stronger but also the scion is basically vertical upwards, and crown inclination cannot occur after the scion survives; only branches, a terminal bud and 4-5 leaves of the scion are kept, so that the transpiration of the blades is reduced, and the moisture loss is reduced. According to the joint grafting technology disclosed by the invention, only the terminal bud, which is about 1cm, of the scion is kept, and thus, the distance from the stock to the terminal bud is shortened, the sprouting capability of the terminal bud is enhanced, and the grafting survival rate reaches up to 98 percent. A Torreya grandis grafted plant is upright; the crown does not incline; the grouting capability is strong; the crown can be rapidly formed.
Owner:胡冬益 +2

Adjustable white celery soil hilling device

The invention discloses an adjustable white celery soil hilling device which comprises two spiral soil borrowing and feeding mechanisms, two soil hilling molds, two fixing racks, soil hilling height adjusting mechanisms, a soil hilling width adjusting mechanism, two compacting and seedling gathering mechanisms and two soil hilling foundation compacting plates. The spiral soil borrowing and feeding mechanisms are fixed to the front ends of the two fixing racks, the two soil hilling molds are arranged on the inner sides of the spiral soil borrowing and feeding mechanisms, the soil hilling height adjusting mechanisms are symmetrically fixed to the two fixing racks, and the soil hilling width adjusting mechanism comprises two adjusting seats and two adjusting cross beams. The two compacting and seedling gathering mechanisms are symmetrically fixed to the front sides of the soil hilling molds, and the two soil hilling foundation compacting plates are fixed below the outer sides of the two soil hilling molds. The adjustable white celery soil hilling device can simultaneously adjust the width and height of white celery soil hilling and is simple in structure and convenient to operate. Soil block compacting plates can compact big soil blocks on two sides of seedlings, compact soil and ensure firm soil hilling, and seedling gathering frames can gather and centralize white celery seedlings lodging on two sides of the seedlings.
Owner:溧阳市易佳甸园生态农业发展有限公司

Tobacco package fertilization method

The invention relates to a tobacco package fertilization method, which is mainly characterized in that three kinds of fertilizers: special tobacco active biological organic and inorganic fertilizers, special tobacco seedling extracting fertilizers and special tobacco additional fertilizers are adopted, and the fertilizers with different nutrition mixture ratios are adopted according to different growth periods of the tobacco for promoting the growth of the tobacco. The tobacco package fertilization method is guided by a tobacco fertilization demanding rule, different forms of fertilizers withdifferent nutrients are applied in different field growth periods of the tobacco for promoting the tobacco to realize the better growth, and the fertilization method has the effects of simplifying the tobacco farmer work procedure, reasonably balancing the tobacco fertilization, improving the tobacco use value and the like, so the goals of reducing the work intensity of tobacco farmers, enhancingthe efficiency, improving the quality of the tobacco leaves and increasing the yield are reached.
Owner:湖南金叶众望科技股份有限公司

Method for raising tea seedlings by cutting in winner

The invention provides a method for raising tea seedlings by cutting in winner and relates to the technical field of tea seedling raising. The method is characterized by comprising the following steps of twig picking, wherein semi-lignification twigs reserved in a female parent garden are picked; twig soaking, wherein the picked twigs are placed in a soak solution for soaking, the soak solution is prepared from, by weight, 4-6 parts of sepiolite powder, 20-50 parts of purified water, 1-2 parts of nonylphenol polyoxyethylene ether, 3-4 parts of tartaric acid and 1-3 parts of potassium oxalate; twig airing, wherein the twigs obtained after soaking are aired in the sun, and the content of moisture in the twigs obtained after airing ranges from 30 to 40; cuttage and scissoring, wherein a cuttage method comprises the steps that the cuttage plant spacing is 3 centimeters, the line spacing is 8 centimeters, the number of cuttage plants per mu ranges from 220,000 to 250,000, two thirds of each plant is inserted into soil, buds and petiole are exposed out of the soil surface, soil around cuttings is compacted, and watering and cuttage are conducted simultaneously. By means of the method, moisture is controlled reasonably, the survival rate of the cuttings is high, and later-period growth is promoted.
Owner:郎溪县姚村乡盛茂贵茶叶种植家庭农场

Water planting implantation method without disease and pest applying to spinach

The invention discloses a water planting implantation method without disease and pest applying to spinach, which comprises the following steps: A, vernalization, B, sowing and C, transplanting. The formula of seedling liquid includes 1.2Mumol / L of manganese sulfate, 0.5Mumol / L of ammonium aluminate, 0.6Mumol / L of cobalt chloride, 0.1Mumol / L of cobalt chloride and nickel chloride, 0.2mmol / L of amino-laevulic acid, 1mmol / L of ammonium sulfate, 3g / L of haydite and 25mg / L of medicinal powder. With the water planting implantation method without disease and pest applying to spinach, diseases and pest damages can be prevented, nutritious, safe, environmental-friendly and high-quality spinach can be obtained.
Owner:马山盛世生态种养专业合作社

Human primary hepatocyte separation and culture method

InactiveCN108070551AHigh number of cellsLess damaging to cellsCell dissociation methodsArtificial cell constructsRemove bloodChemistry
The invention provides a human primary hepatocyte separation and culture method. The method comprises the following steps of (1) cutting a fresh human hepatocyte under an aseptic condition, sealing at4 DEG C and then carrying back to a lab; (2) placing the tissue into a precooled sterile PBS buffer liquid containing double antibodies, and washing for multiple times to remove blood and connectivetissues; (3) irrigating through a preheated PBS buffer liquid through a needle-punching method; (4) irrigating through a preheated HBSS buffer liquid through a needle-punching method; (5) irrigating through a preheated GBSS mixed enzyme liquid through a needle-punching method; (6) cutting up the tissue, and using the GBSS mixed enzyme liquid for digesting, centrifuging, and removing a supernatant;(7) using trypan-blue for dyeing a cell filter liquor to detect cell activity; (8) after carrying out cell counting, resuspending a complete medium, inoculating into a coated culture flask, and culturing at 37 DEG C in an environment with 5 percent of CO2; (9) identifying cell morphology; (10) detecting through an ELISA (Enzyme-linked Immuno Sorbent Assay) method; (11) detecting through RT-PCR (Reverse Transcription-Polymerase Chain Reaction). The invention provides the human primary hepatocyte separation and culture method which is simple to operate, the number of the obtained cells is large, the survival rate is high, the method is the ideal human primary hepatocyte separation and culture method, and a reliable cell resource is provided for experiments.
Owner:JIANGYIN CHI SCI

Plant growth regulating composition containing brassin and S abscisic acid

The invention discloses a plant growth regulating composition containing brassin and S abscisic acid, wherein the plant growth regulating composition comprises 0.0001-0.1% of brassin, 0.01-3% of S abscisic acid, a surfactant, 20-50 g of urea, 10-30 g of a trace element fertilizer, 20-50 g of water-soluble polyvinyl butyral and 100 g of water. According to the invention, various plant growth regulating components are loaded and combined through a high polymer material, brassin with high activity and S abscisic acid synergistically induce and regulate the anti-freezing performance of plants, anda proper amount of amino acids and trace elements are matched to intervene in the growth of the plants in the anti-freezing induction process, so that the plants have good environmental adaptabilityin different growth stages, and the later growth of the plants is effectively promoted while the freezing resistance of the plants is rapidly improved.
Owner:河南省莱恩坪安园林植保有限公司

Method for planting oilseed rape

The invention discloses a method for planting oilseed rape. The method comprises the following steps that a, sunny and fertile land convenient to irrigate is selected as a seedling growing area, weedsare removed, the soil is turned, seedling growing beds with the height of 10 cm to 15 cm are accumulated, and drainage ditches of 20 cm to 30 cm are reserved between the seedling growing beds; b, base fertilizer is applied, wherein 250 kg to 300 kg of compound fertilizer is applied per mu; c, seed mixing is carried out, oilseed rape seeds and a culture medium are mixed, and an oilseed rape seed medium is obtained; d, a proper amount of clear water is added into the oilseed rape seed medium to be mixed to be uniform, the temperature of the oilseed rape seed medium is kept between 22 DEG C to 25 DEG C, and the oilseed rape seed medium is turned till the oilseed rape seeds germinate to become seedlings with 1-2 leaves; e, seedlings are transplanted into the seedling growing beds, and a proper amount of clear water is applied to the seedling growing beds; f, after the seedlings are cultivated in the seedling growing beds for 25-30 days, a proper amount of leaf fertilizer is applied to theseedlings till the leaves of the seedlings grow to become 5-7, and the seedlings are transplanted; g, the seedlings in the growing period are topdressed with base fertilizer; h, oilseed rape seeds are mature and are harvested. The germination rate of the oilseed rape seeds is increased, and meanwhile the survival rate of the seedlings is increased.
Owner:四川巴蜀粮仓科技有限责任公司

Method for improving rhus succedanea seed germination rate and accelerating germination

The invention discloses a method for improving the rhus succedanea seed germination rate and accelerating germination. The method comprises the following steps that (1) ripe and full rhus succedanea seeds are selected to get peels removed and disinfected, and then the treated seeds are dipped with 98% concentrated sulfuric acid for 20-25 minutes so as to accelerate germination; and (2) the seeds obtained in the step (1) are dipped with a gibberellin solution with the concentration being 300mg / L for 12 hours, the seeds are washed cleanly under flowing water after being treated, and the cultureconditions are controlled to conduct germination acceleration. The method can omit the steps of long-time seed soaking and stratification treatment, rapidly breaks seed dormancy of rhus succedanea, effectively improves the seed germination rate, and the germination rate is as high as 98% or above. Meanwhile, the germination time is greatly shortened, the seeds can germinate completely after about7-10 days, the purpose of rapid gemination acceleration is achieved, germination is tidy and consistent, and operation management such as seedling transplanting and field planting in the following period is facilitated.
Owner:WUHAN ACADEMY OF AGRI SCI
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