The invention aims at solving the problems that seed propagation adopted in current
polygonatum sibiricum red production is difficult,
plant diseases and
insect pests can easily become more serious and
germplasm can be easily degenerated during the conventional
rhizome propagation,
tissue culture mainly takes terminal buds of
rhizome and stem tips as explants for establishing a rapid propagation
system, source of the explant is single,
test material consumption is high and propagation efficiency is low. According to the method provided by the invention, easily available
polygonatum kingianum terminal
bud scales are selected as an explant
test material, adventitious roots are obtained by inducing
bud scale differentiation, and then further induction is carried out on the adventitious roots, so that differentiation bodies of different types such as rhizomes having adventitious roots and terminal buds at the same time, adventitious
bud clumps and
callus tissues in
multiple forms are obtained; and respectively continuously culturing the differentiation bodies of different types, so that new adventitious bud clumps, rhizomes with terminal buds and
callus tissues of multiple types can be respectively continuously produced, and an
in vitro high-frequency regeneration
system for roots, stems and leaves of
polygonatum sibiricum red is established; a high-frequency regeneration
system for the roots and stems can also be obtained by adopting the same culture medium. The roots, stems, leaves,
callus tissues and rhizomes obtained by utilizing the method can be mutually transformed and grow in different culture stages.