The invention discloses an IL-6
immunoturbidimetry detection kit prepared based on a recombinant
monoclonal antibody. The IL-6
immunoturbidimetry detection kit comprises a
reagent R1 and a
reagent R2.Two prepared anti-IL-6
antibody latex
microsphere mother solutions are used as one component of
reagent R2 of the kit, PEG 6000 is added to the reagent R1 according to a certain proportion, the reagent R1 is composed of a
buffer solution, a surfactant, a stabilizer and a
preservative, and the reagent R2 is composed of a
buffer solution, a surfactant, a stabilizer, a
preservative and an
antibody.The invention also discloses a preparation method of the IL-6
immunoturbidimetry detection kit prepared on the basis of the recombinant
monoclonal antibody. Compared with a similar kit for determiningIL-6 by latex enhanced immunotransmission
turbidimetry, the kit disclosed by the invention adopts a mode of combining two anti-human IL-6
monoclonal antibodies, can be used for respectively identifying two
antigen epitopes, has higher sensitivity than a single
monoclonal antibody kit, and has better specificity than a polyclonal antibody kit.