The invention discloses a multifunctional separation sieve mesh preparation method, which comprises: S1, pre-treating, wherein a sieve mesh is placed and washed in a cleaning agent, rinsing is performed with ultrapure water, and drying is performed so as to be spare; S2, soaking the sieve mesh pretreated in the step S1 in a polymer coating solution; S3, hanging and drying the sieve mesh coated inthe step S2, and soaking in an acidic glutaraldehyde solution; S4, taking out the sieve mesh obtained in the step S3, drying, and soaking in an antibacterial surfactant solution; and S5, taking out the sieve mesh soaked in the step S4, and drying to obtain the super-hydrophilic and super-oleophobic sieve mesh. According to the invention, the prepared sieve mesh has super-hydrophilic and super-oleophobic performance, can effectively achieve oil-water separation, has multiple functional groups contained in the coating on the surface of the sieve mesh, has excellent oil-water separation performance, further has performances of heavy metal ion adsorbing, antibacterial property and the like, and has substantially expanded application range.