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The invention discloses a strain of Paenibacillus polymyxa and its application in preventing and treating various soil-borne diseases. The preservation number of Paenibacillus polymyxa ZF129 protected by the present invention is CGMCC No.17631. Paenibacillus polymyxa (Paenibacillus polymyxa) ZF129 can significantly inhibit the growth of pathogenic fungal hyphae, display broad-spectrum antagonistic activity, and have growth-promoting ability to plants. Paenibacillus polymyxa (Paenibacillus polymyxa) ZF129 exhibits excellent biocontrol properties, has potential for further development, and lays a foundation for in-depth research on the mechanism of Paenibacillus polymyxa in disease prevention.

The invention discloses an application of N-acetylated chitohexaose for inhibiting the proliferation of tumor cells. The sugar residue at the reducing end of the N-acetylated chitohexaose is N-acetylglucosamine and the sugar residue at the 2th site from the reducing end has a glucosamine structure. A natural non-toxic chitosan oligosaccharide is used as a lead compound and through selective N-acetylation and ion exchange chromatography separation, a specific structural component of N-acetylated chitohexaose having strong anti-tumor activity is screened. The N-acetylated chitohexaose has a great significance to develop efficient and low-toxic marine anti-tumor innovative drugs.

A method of extracting dibutyl phthalate from common buried rubber is provided. The method includes weighing the common buried rubber, performing ultrafine smashing, adding ethyl acetate, extracting, centrifuging, precipitating, adding ethanol having a concentration of 95%, extracting, centrifuging to obtain a supernatant liquid, concentrating the supernatant liquid, drying, adding an ethanol water solution having a concentration of 80%, dissolving under the function of ultrasonic waves until the concentration is 15-50 mg / mL, and separating through a C18 reverse phase silica gel chromatographic column, wherein a binary mobile phase adopting purified water as an A phase and methanol as a B phase is adopted; elution is performed in a manner that the mobile phase in which the concentration of the methanol is 20% is adopted for 5 min, then the mobile phase in which the concentration of the methanol is 40% is adopted for 7 min, then the mobile phase in which the concentration of the methanol is 80% is adopted for 13 min and finally the mobile phase in which the concentration of the methanol is 100% is adopted for 5 min; and eluate from the 21 min to the 22 min is collected according to an ultraviolet absorption spectrum, concentrated and freeze-dried in vacuum to obtain the dibutyl phthalate the purity of which is 99% or above. The method is stable in separation, high in repeatability, high in preparation amount and simple and time-saving in operation.