The invention relates to the technical field of bread, in particular to mushroom bread and a preparation method of the mushroom bread. The mushroom bread is prepared from the following components: 100 g of bread flour, edible salt in the mass 0.5%-2% that of the bread flour, a bread improver in the mass 0.4%-1% that of the bread flour, butter in the mass 5%-8% that of the bread flour, saccharose in the mass 12%-18% that of the bread flour, active dry yeast in the mass 1.8%-2.2% that of the bread flour, minced mushroom in the mass 1.2%-1.7% that of the bread flour and mushroom juice in the mass 40%-50% that of the bread flour. Fresh mushroom is boiled in water, the mixture is minced firstly and then separated or separated firstly and then minced, and the mushroom juice and the minced mushroom are obtained; the baume degree of the mushroom juice is 1-10 baume degrees, the content of water in the minced mushroom is 20%-30%, and the average particle size of the minced mushroom is 2-4 mm; the mushroom bread is fermented at the temperature of 30-34 DEG C for 43-48 min, then shaped, proved and baked at the temperature of 175-185 DEG C for 18-22 min, and the mushroom bread is prepared. The mushroom bread has the characteristics of good texture, fullness, good glossiness, soft hand feel, proper toughness, softness, palatability, moderate sweetness, moderate bread flavor and mushroom flavor and the like and is nutritious mushroom bread with good sensory indexes and strong flavor.