The invention discloses a preparation method of drug-loading hemostatic sponge. The preparation method comprises the following steps: (1) preparing lignocelluloses sponge; and (2) loading the lignocelluloses sponge with chitosan and a hemostatic drug in a spraying or immersion manner, and freeze drying to obtain the drug-loading hemostatic sponge. A preparation method of the lignocelluloses spongecomprises the following steps: (1) freezing an alkali urea solution, forming icy slurry, adding lignocelluloses into the icy slurry, and obtaining a lignocelluloses solution; (2) adding a surfactantinto the lignocelluloses solution, stirring, and forming a foam body; (3) freezing the foam body into foam icy slurry, adding a pore forming agent into the foam icy slurry, injection molding, freezing, aging, and obtaining gel; and (4) soaking to remove the surfactant and the pore forming agent in the gel, freeze shaping, freeze drying, and obtaining the lignocelluloses sponge. By adopting the drug-loading hemostatic sponge prepared by the invention, by virtue of the synergistic effect of the chitosan and hemostatic drug, the blood on the surface of a wound can be rapidly absorbed, the blood coagulation of the wound can be effectively promoted, and the bleeding can be rapidly stopped.