The invention discloses a method for preparing an
antifungal pectin oligosaccharide through citrus peel. The method includes the steps that
raw material preprocessing
pectin is extracted, and the
pectin is hydrolyzed through an enzymic method, wherein the optimal conditions of pectin
hydrolysis achieved through the enzymic method include that a pectin solution with the
mass concentration being 1.0-1.1% is prepared from an
acetic acid-
sodium acetate buffering solution with the pH being 4.5,
pectinase P21 is added, the adding amount of
pectinase is 1.0-1.1U / mL, a stirring reaction is carried out for 20-50 min through water bath at the temperature of 40-50 DEG C, and then
enzyme denaturalization is carried out for 5-15 min in boiling water bath. The pectin
oligosaccharide prepared through the preparation method is mainly
disaccharide and
trisaccharide, and the yield is larger than 52%. The pectin
oligosaccharide has an obvious
antifungal effect and has a good inhibiting function on four common pathogenic fungi. The pectin oligosaccharide is prepared into oligosaccharide
composite film liquid, is used for citrus preservation, prolongs
citrus fruit storage time easily, and lays a foundation for further utilization of the pectin oligosaccharide. The additional value of citruses is improved, environment
pollution can also be reduced, and the oligosaccharide is prepared from the citruses and is used for the citruses.