Belonging to the field of biotechnologies, the invention discloses an expression vector containing regulatably expressible cre recombinase gene and reporter gene LacZ, a construction method and application thereof. The expression vector provided by the invention is pBI-3-LacZ-cre, and the expression vector contains the lacZ reporter gene, the Cre recombinase gene and a bidirectional promoter tetO element. According to the invention, the expression vector pBI-3-LacZ-cre is introduced into a mouse fallopian tube to conduct pregnancy so as to obtain a transgenic mouse. The transgenic mouse is hybridized with two applied model animals, i.e. an rTALAP-1 mouse able to induce liver specific expression of rtTA molecules and an HBV Tg (Transgenic) mouse able to regulatably express HBV genome, and then after repeated mating, a liver inducibly expressible Tet-on and Cre / loxP combined dual-control strategy triple-transgenic mouse new model can be obtained finally. The expression vector can be effectively applied to screening and evaluation of HBV (hepatitis B virus) inhibitors or drugs, research of HBV pathogenic mechanism, host immune response after HBV infection and other key issues.