The invention discloses a detection method for the trinexapac-ethyl residual quantity in a
high performance liquid chromatography determination environmental sample. The detection method for the trinexapac-ethyl residual quantity in the
high performance liquid chromatography determination environmental sample comprises the following steps: (1) a
water sample is enriched by a
hydrophile-lipophile balance (HLB)
solid phase extraction column, eluted by carbinol, and concentrated with a constant volume and then
fractionation detection is carried out on the
water sample with a
high performance liquid chromatography system; (2) a soil sample is wet with water, extracted in a vibrating mode by
acetonitrile and layered through
salting out, an organic phase is concentrated with a constant volume, and then
fractionation detection is carried out on the soil sample with the high performance liquid
chromatography system. Octadecyl
silane is adopted as a filling agent by a
chromatographic column, and a
detector is a
ultraviolet detector with a
diode array. A mobile phase is a mixed
solvent of carbinol and a 0.1%
phosphoric acid aqueous solution, the ratio is that, by volume, the carbinol accounts for 50%-70%, and the 0.1%
phosphoric acid aqueous solution accounts for 50%-30%. The detection
wavelength is 280 nm, the temperature of the column is 25 DEG C to 40 DEG C, and the injected
sample volume is 10-20 mu L. Through systematic comparative study, the detection method for the trinexapac-ethyl residual quantity in the high performance liquid
chromatography determination environmental sample is created. The detection method for the trinexapac-ethyl residual quantity in the high performance liquid
chromatography determination environmental sample has the technical advantages of being good in
fractionation effect, accurate in determination, high in sensitivity, strong in specificity, convenient and quick to analyze and the like.