38 results about "Ochrobactrum species" patented technology

The invention discloses an immobilization method of a salt-tolerant and cold-tolerant compound strain and an application of the immobilization method. The immobilization method of the salt-tolerant and cold-tolerant compound strain comprises the following steps: firstly screening efficient anaerobic ammonia oxidation bacteria, denitrifying bacteria and petroleum-degrading bacteria, training salt tolerance and cold tolerance to respectively prepare trained Kuenenia. Anammoxidans, Ochrobactrum.spand Flavobacterium mizutaii; putting prepared biological carbon spheres into a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria enrichment solution, culturing films for the biological carbon spheres and subsequently mixing the degrading bacteria enrichment solution with a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria embedding compound solution, finally putting the biological carbon spheres in the mixed solution into a saturated borate solution and a 2%CaCl2 mixed solution one by one, then putting the biological carbon spheres into a refrigerator, crosslinking an washing to obtain salt-tolerant and cold-tolerant compound strain immobilization biological carbon spheres. The salt-tolerant and cold-tolerant compound strain is applied to purification of ammonia nitrogen and water polluted by petroleum hydrocarbon under the condition with low temperature and high salt, so that the strain is high in content of microbial biomass; obviously, the biological carbon spheres are used as carriers for immobilizing the salt-tolerant and cold-tolerant compound strain; the carriers are wide in material resources, simple to manufacture, suitable for industrialization, high in mechanical strength, excellent in treatment effect, low in cost and free of secondary pollution to the environment.

The invention discloses a method for separating denitrification desulfurizing bacteria based on biodiversity information and relates to denitrification desulfurizing bacterium screening methods. The method comprises the steps of collecting sludge, conducting high-throughput sequencing on the sludge sample, analyzing the flora abundance fluctuation and space ecological distribution of the sludge sample, adopting different screening methods according to community composite and structure characteristics, conducting anaerobic culture on a target bacterial genus by means of an interlayer culture medium with different screening methods, selecting a single colony for isolated culture, and obtaining a single bacterial colony after repeated operation. Two kinds of denitrification desulfurizing bacterial strains, namely autotrophic denitrification desulfurizing bacteria which belong to Thiobacillus, and heterotrophic denitrification desulfurizing bacteria which belong to Thauera, Azoarcus, Arcobacter and Ochrobactrum are obtained through screening. Community structure information displayed by the strain screening result is consistent with that displayed by the high-throughput sequencing result, microbial resources are provided for improving biological treatment effectiveness of wastewater. Furthermore, the metabolic characteristics of the strains are identified.

The invention provides a degradation bacillus which can dispel the phoxime pesticide residue and its prodegradant. The used strain is Gram's staining reaction hysteroptosis X-12 which is Ochrobactrum.sp. The main biology characteristic is G-; the thaliana is the rod type whose size is (1.58-2.61)mu m*(0.79-1.23)mu m; it has flagellation around it and facultative anaerobes; the hydrogen peroxidase, oxidative enzyme, oxidation of ethanol and V.P. reaction is positive; the ketole reaction is negative; it can not be hydrolysed starched and oxide glucose to generate the acid so that the coagulate dydimic acid cow's milk; the Genbank landing number of the strain 16S rDNA is DQ093374.

The invention belongs to the technical field of biology, and relates to a bacterial strain for high-producing bacterial polysaccharide and a method thereof for preparing polysaccharide by fermenting traditional Chinese medicinal waste. The bacterial strain is preserved in the China Center for Type Culture Collection (CCTCC); the preservation number is CCTCC M 2016273. A bacterial strain of intermediate ochrobactrum ZY03 is inoculated in a culture medium with a sucrose content of 10g / L; the yield of the polysaccharide can reach 6g / L at most; the conversion ratio can reach 67 percent at most.

The invention relates to a degrading bacteria which can effectively degrade a disinfectant of chlorothalonil. The classified name of the degrading bacteria is: Ochrobactrum lupine which is preserved in China General Microbiological Culture Collection Center in June the 28th, 2007 and the preservation number thereof is CGMCC No.2098. The degrading bacteria is used to degrade the disinfectant of chlorothalonil and can be used for the biological purifying of water, soil or agricultural products polluted by the chlorothalonil.

The invention relates to a pesticide carbendazim degrading microbial inoculant and a preparation method thereof. In an agricultural production process, as pesticides are excessively used, high residues of pesticides in agricultural products and agricultural soil are caused, which brings a certain problem to safety of the agricultural products and sustainable development of the agricultural soil. A human ochrobactrum WNPA2 is cultured in a special liquid fermenting culture medium, fermentation liquor is collected for centrifugal separation to remove moisture in the fermenting liquor to obtain milk white thick somatic cells; by adding glycerinum and diatomite which are uniformly stirred and mixed, a white solid preparation, namely, the target product, is obtained. The preparation method provided by the invention is an efficient and economical environment-friendly pesticide residue treatment technology. The carbendazim degrading rate can reach 50% above, and the pesticide carbendazim degrading microbial inoculant has the advantages of being low in treatment cost, simple to construct, free of secondary pollution, small in environmental influence, etc.

The invention belongs to the technical field of biology and particularly discloses a method for efficiently degrading azoxystrobin by utilizing ochrobactrum anthropic. Ochrobactrum anthropic SH14 is used for degrading the azoxystrobin under the condition that the temperature is 30.2 DEG C, the pH is 7.9 and the inoculum size is 0.2g / L, and the degradation rate of the ochrobactrum anthropic SH14 on 50mg / L of azoxystrobin is above 85%; the method disclosed by the invention provides theoretical practice basis for future large-scale industrial production and application of the ochrobactrum anthropic SH14 and has wide signification.

A triazophosphorus pesticide residual degrading bacteria and bacteria preparation produced from it are disclosed, the strain is Gram's chromatic reacting negative bacteria strain MP-4, which belongs to ochrobactrum.sp. Its characteristics are G-, nemaline, 1.58X0.79mum; reaction of oxidase, glycolic oxidation and V.P is positive; indole reaction is negative; Genbank number of the strain 16S rDNA is AY331578. Its advantages include decrement of pesticide residual amount, atoxic and innoxious green agricultural product.

The invention relates to ochrobactrum and application thereof and belongs to the technical field of applied microbiology. The ochrobactrum sp.4-40 disclosed by the invention was preserved in CGMCC (China General Microbiological Culture Collection) on 3rd, November, 2011 with the preservation number of CGMCC No.5442. The ochrobactrum sp.4-40 has the capability of metabolizing nicotine and can growat the concentration of the nicotine as high as 4.5g / L. By culturing the ochrobactrum sp.4-40 in a large amount, a biocatalyst using a complete cell of the strain is used for completely degrading pure nicotine and can be used for reducing the content of the nicotine in tobacco leaves simultaneously. The ochrobactrum sp.4-40 disclosed by the invention has the advantages that the nicotine in tobacco and tobacco wastes can be degraded and the harm of cigarettes on a human body and the pollution to the environment are reduced.

The invention discloses Ochrobactrum CTS-325 and a culturing method thereof as well as application to reducing hexavalent chromium, and relates to microbe and application thereof to efficiently and thoroughly reducing the hexavalent chromium under the aerobic condition as well as a culturing method thereof. The Ochrobactrum CTS-325 has a preserving number CGMCC1715. The strain can efficiently andthoroughly reduce the hexavalent chromium. The pH value is between 7 and 9 during reduction of the hexavalent chromium by the strain. The strain comprises one or combination of any few kinds of xylose, ribose, glucose, levulose, galactose, sucrose, maltose, starch, glycerin or mannite in an available culture medium on microbiology. When industrial waste water containing chromium and chromium slag leach liquor are treated by the Ochrobactrum CTS-325 in an optimized culture medium, the hexavalent chromium in the industrial waste water and the chromium slag leach liquor can be reduced to reach sewage discharge standard.

The invention discloses wheat ochrobactrum of degradable lincomycin. The wheat ochrobactrum is preserved in China General Microbiological Culture Collection Center as the preservation number CGMCC No.10665. The wheat ochrobactrum of the degradable lincomycin can use the lincomycin as an exclusive carbon source to grow, and the degradation capacity of the strain for the lincomycin can reach 100 percent in 24 hours. The strain can be used for harmlessly treating fermented fungi residue in the industrial production of the lincomycin, the treatment method is effective, environment-friendly, capable of saving the resource, low in treatment cost and capable of thoroughly eliminating adverse impact of lincomycin waste hypha on the environment.

The invention provides a triazophos pesticide residue degrading bacterium and the bacterial agent produced therefrom. The used bacterial strain is Gram staining negative bacterial strain MP-4, which is identified as Ochrobactrum.sp. The main biological characteristic is G - , the bacteria are rod-shaped, about 1.58×0.79μm in size, with periosteum flagella, facultative anaerobic; catalase, oxidase, ethanol oxidation and V.P. reactions are positive; indole reaction is negative; starch cannot be hydrolyzed, glucose is oxidized Produce acid to coagulate litmus lactic acid. The Genbank accession number of the strain 16S rDNA is AY331578. The direct application of degrading bacteria products can reduce the amount of pesticide residues in crops by more than 90%, solve the problem of excessive pesticide residues in agricultural production, and produce non-toxic and pollution-free green agricultural products.

The invention provides functional protein capable of degrading chrysene. The functional protein capable of catalyzing and degrading the chrysene is obtained by extracting and purifying a bacterium liquid of Ochrobactrum anthropic DW1 with the strain preservation number of CGMCC NO.8621. When the functional protein catalyzes and degrades the chrysene, the proper pH value ranges from 6.0 to 8.0, the most proper pH value is 7.0, the functional protein can be deactivated and denaturized easily when the pH value is smaller than 4 or larger than 10, the proper reaction temperature ranges from 30 DEG C to 45 DEG C, the most proper reaction temperature is 35 DEG C, the functional protein can be deactivated and denaturized easily when the temperature is higher than 50 DEG C, and Cu<2+>, Ni<2+> and Hg<2+> ions with the concentration of 1 mmol / L have stronger inhibition effects on the activity of the functional protein. The functional protein can effectively catalyze and degrade the chrysene and has the application potential for soil and water polluted by the polycyclic aromatic hydrocarbon chrysene.