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Pesticide carbendazim degrading microbial agent and preparation method thereof

A microbial agent and carbendazim technology, which is applied in the field of pesticide carbendazim degradation microbial agent and its preparation, can solve the problems of unreasonable use of chemical pesticides, ecological environment pollution, excessive pesticide residues, etc., and achieve simple construction and environmental protection. The effect of small impact and low processing cost

Active Publication Date: 2019-10-25
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in the use of chemical pesticides are irrational use of chemical pesticides, serious pesticide residues in agricultural products, high cost of control, serious pollution to the ecological environment, and large-scale pesticide damage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] The preparation method of the pesticide carbendazim degrading microbial bacterial agent that the present invention relates to comprises the following steps:

[0021] Step 1: Fermentation:

[0022] Adjust the pH of the liquid fermentation medium A to 6.5-7.0, and carry out routine aseptic treatment, and insert Pallidum pallidum WNPA2 according to the aseptic operation of liquid mass 6-9%, constant temperature 30±1°C, stirring speed 180-200rpm, Ventilation 0.27-0.30M 3 / min, tank pressure 0.07-0.08MPa, continuous fermentation 72-76hr, microscopic detection and counting the number of cells in the fermentation liquid reached 1.5-2.0×10 9 Individual / mL to stop the fermentation;

[0023] Step 2: Separation of bacterial cells:

[0024] Collect the Paleobacterium pallalis WNPA2 fermentation liquid of the liquid fermentation medium A in step 1, and centrifuge to remove the water in the fermentation liquid with a yeast separation centrifuge to obtain milky white viscous bacter...

Embodiment 1

[0031] Step 1: Fermentation:

[0032] Adjust the pH of the liquid fermentation medium A to 6.0, and carry out conventional aseptic treatment, and insert Pallidum pallidum WNPA2 according to the aseptic operation of 9% liquid mass, with a constant temperature of 29°C, a stirring speed of 180rpm, and a ventilation volume of 0.27M 3 / min, tank pressure 0.08MPa, continuous fermentation for 74hrs, and the number of cells in the fermentation liquid reached 2.0×10 by microscope detection and counting 9 Individual / mL to stop the fermentation;

[0033] The formula of liquid fermentation medium A is as follows:

[0034] Glucose 18g / L, MgSO 4 ·7H 2 O 0.5 g / L, NH 4 NO 3 1.0 g / L, (NH 4 ) 2 SO 4 1.5g / L, NaCl1.0g / L, K 2 HPO4·3H 2 O 0.5 g / L, MnSO 4 2H 2 O 0.4mg / L, and make the remaining water to 1L.

[0035] Step 2: Separation of bacterial cells:

[0036] Collect the Paleobacterium pallalis WNPA2 fermentation liquid in the liquid fermentation medium A in step 1, centrifuge the ...

Embodiment 2

[0040] Step 1: Fermentation:

[0041] Adjust the pH of the liquid fermentation medium A to 6.2, and carry out routine aseptic treatment, and insert Pallidum pallidum WNPA2 according to the aseptic operation of 6% of the liquid mass, with a constant temperature of 31°C, a stirring speed of 190rpm, and a ventilation volume of 0.28M 3 / min, tank pressure 0.08MPa, continuous fermentation for 76hrs, the number of cells in the fermentation liquid reached 1.8×10 9 Individual / mL to stop the fermentation;

[0042] The formula of liquid fermentation medium A is as follows:

[0043] Glucose 15g / L, MgSO 4 ·7H 2 O 0.6 g / L, NH 4 NO 3 1.5 g / L, (NH 4 ) 2 SO 4 2.0 g / L, NaCl1.0g / L, K 2 HPO4·3H 2O 0.8g / L, MnSO 4 2H 2 O 0.5mg / L, and make the remaining water to 1L.

[0044] Step 2: Separation of bacterial cells:

[0045] Collect the Paleobacterium pallalis WNPA2 fermentation liquid of the liquid fermentation medium A in step 1, and centrifuge to remove the water in the fermentation ...

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PUM

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Abstract

The invention relates to a pesticide carbendazim degrading microbial inoculant and a preparation method thereof. In an agricultural production process, as pesticides are excessively used, high residues of pesticides in agricultural products and agricultural soil are caused, which brings a certain problem to safety of the agricultural products and sustainable development of the agricultural soil. A human ochrobactrum WNPA2 is cultured in a special liquid fermenting culture medium, fermentation liquor is collected for centrifugal separation to remove moisture in the fermenting liquor to obtain milk white thick somatic cells; by adding glycerinum and diatomite which are uniformly stirred and mixed, a white solid preparation, namely, the target product, is obtained. The preparation method provided by the invention is an efficient and economical environment-friendly pesticide residue treatment technology. The carbendazim degrading rate can reach 50% above, and the pesticide carbendazim degrading microbial inoculant has the advantages of being low in treatment cost, simple to construct, free of secondary pollution, small in environmental influence, etc.

Description

technical field [0001] The invention belongs to the technical field of soil bioremediation, and in particular relates to a pesticide carbendazim-degrading microbial bacterial agent and a preparation method thereof. Background technique [0002] As an important means to ensure a good agricultural harvest, chemical pesticides play an important role in agricultural production. The main problems in the use of chemical pesticides are irrational use of chemical pesticides, serious pesticide residues in agricultural products, high control costs, serious pollution to the ecological environment, and large-scale phytotoxicity. Microbial remediation is a biological measure that utilizes the metabolic absorption, transformation, removal or degradation of chemical pesticide components of specific microorganisms to achieve environmental purification and ecological restoration. It has the characteristics of high efficiency, economy, and no secondary pollution. This technology has been ap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/40C12N1/20C09K101/00C12R1/01
CPCC09K17/40C09K2101/00C12N1/20
Inventor 陈锐孙晓宇沈卫荣路鹏鹏赵玲侠瞿佳
Owner MICROBIOLOGY INST OF SHAANXI
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