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Ochrobactrum ZJUTCB-1 capable of efficiently degrading chlorobenzene and application of ochrobactrum ZJUTCB-1

A technology of ZJUTCB-1 and Pallidum, which is applied in the field of biological treatment of environmental pollutants, can solve the problems of maintaining high efficiency, not meeting high-efficiency production, and low degradation rate, and achieve low purification cost, high-efficiency purification, and low pollution.

Active Publication Date: 2019-02-22
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1980s, a large number of scholars at home and abroad have screened out some chlorobenzene-degrading microorganisms from sludge, water, and soil, including Psudomonas sp.strain WR1306, P.putida&Bacillus sp., Escherichiahermanii, Pseudomonas sp.strain JS150, Ralstonia sp. .strain JS705, Aeromonas sp., Pseudomonas sp.strain FY01&FY04, Bacillus sp.Strain FY02&FY03, Pseudononas sp.Nov.LP01, etc., the above microorganisms can degrade the pollutant under aerobic or anaerobic conditions, but not in aerobic conditions at the same time Maintain high efficiency under and anaerobic conditions, and degradation rate is low (under aerobic and anaerobic conditions, the degradation rate of p-chlorobenzene in 18d is respectively 50% and 37.2%), does not meet the requirement of industrial high-efficiency production

Method used

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  • Ochrobactrum ZJUTCB-1 capable of efficiently degrading chlorobenzene and application of ochrobactrum ZJUTCB-1
  • Ochrobactrum ZJUTCB-1 capable of efficiently degrading chlorobenzene and application of ochrobactrum ZJUTCB-1
  • Ochrobactrum ZJUTCB-1 capable of efficiently degrading chlorobenzene and application of ochrobactrum ZJUTCB-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Separation, purification, identification and preservation of Ochrobacterum sp. ZJUTCB-1.

[0021] 1. Screening of Ochrobacterum sp.ZJUTCB-1

[0022] Ochrobacterum sp.ZJUTCB-1 is a Gram-negative bacterium domesticated and isolated from the activated sludge of Hangzhou Qige Wastewater Treatment Plant. Specific steps are as follows:

[0023]Add 200ml of inorganic salt culture solution to a 340mL serum bottle, pass high-purity nitrogen gas (40mL / min) for 30min to remove the dissolved oxygen in the inorganic salt culture solution and the air at the top of the serum bottle, add 20mL of activated sludge and 987μmol / L Chlorobenzene, at 30°C, 160rpm for enrichment culture. When the concentration of chlorobenzene is 50% of the initial concentration, take out 20mL of the enrichment solution and add it to 200mL of fresh inorganic salt culture solution, then add 987μmol / L chlorobenzene, and cultivate anaerobically at 30°C and 160rpm to the concentration of chlorobenzene...

Embodiment 2

[0035] Example 2: Activation of Ochrobacterum sp.ZJUTCB-1 and degradation of different concentrations of chlorobenzene under aerobic conditions

[0036] Under the conditions of optimal environmental factors (culture solution pH=7, culture temperature 30°C), the degradation performance and bacterial growth of the strain ZJUTCB-1 to the initial concentration of 347.31-2604.80 μmol / L chlorobenzene under aerobic conditions were investigated. . The results show that the strain can completely degrade 347.31-2604.80 μmol / L of chlorobenzene, with an average speed of 82.27-174.85 μmol / (L h). After the chlorobenzene is fully utilized, the quality of the bacteria ZJUTCB-1 also increases significantly. The specific implementation Proceed as follows:

[0037] Prepare inorganic salt culture solution (same as Example 1), adjust the pH to 7 with sodium hydroxide or dilute hydrochloric acid solution, and distribute it in serum bottles with an average volume of 340 mL, 50 mL per bottle.

[00...

Embodiment 3

[0041] Example 3: Activation of Ochrobacterum sp.ZJUTCB-1 and degradation of different concentrations of chlorobenzene under anaerobic conditions

[0042] Under the conditions of optimal environmental factors (culture solution pH=7, culture temperature 30°C), the degradation performance and bacterial growth of the strain ZJUTCB-1 to the initial concentration of 347.31-2604.80 μmol / L chlorobenzene under anaerobic conditions were investigated. . The results showed that the strain could completely degrade 347.31-1736.53 μmol / L chlorobenzene with an average rate of 97.39-210.38 μmol / (L h). When the concentration of chlorobenzene was as high as 2604.80 μmol / L, the strain could also complete 88.24 % removal, the average removal rate was 102.47μmol / (L·h). After the chlorobenzene is fully utilized, the quality of the bacteria ZJUTCB-1 also increases significantly, and the specific implementation steps are as follows:

[0043]The preparation of seed solution is the same as in Example...

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Abstract

The invention discloses ochrobactrum ZJUTCB-1 capable of efficiently degrading chlorobenzene and application of the ochrobactrum ZJUTCB-1. Organic pollutants include toluene, p-xylene, the chlorobenzene and 1, 2-dichlorobenzene or n-hexane. A method for applying the ochrobactrum ZJUTCB-1 includes inoculating the ochrobactrum ZJUTCB-1 into inorganic salt culture solution; adding the organic pollutants into the inorganic salt culture solution; carrying out degradation reaction under the conditions of the temperature of 25-35 DEG C, the rotational speed of 160 rpm and the pH (potential of hydrogen) of 6-8; degrading organic matters. The ochrobactrum ZJUTCB-1 and the application have the advantages that the chlorobenzene and other industrial common organic pollutants such as the toluene, the p-xylene, the 1, 2-dichlorobenzene and the n-hexane can be degraded by the ochrobactrum ZJUTCB-1; the chlorobenzene in industrial wastewater and waste gas can be efficiently purified by the ochrobactrum ZJUTCB-1, and the ochrobactrum ZJUTCB-1 is little in pollution, easy to popularize and low in purification cost.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a strain of Ochrobacterum sp. ZJUTCB-1 with high-efficiency degradation ability of chlorobenzene and its application in degrading chlorobenzene. Background technique [0002] Chlorobenzene is the simplest chlorinated aromatic hydrocarbon. It is insoluble in water, has stable physical and chemical properties, and is difficult to degrade naturally. It is widely used in industrial and agricultural production, basic organic solvents and synthesis of organic compounds. It is also used in medicine, dyes, pesticides and engineering plastics important intermediates in the field. Chlorobenzene can stimulate the skin, conjunctiva and respiratory organs of animals, inhibit the nerve center, and damage the liver and kidneys. Chlorobenzene also has the "three causes" effect. Based on the above hazards, chlorobenzene has been listed as a p...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/72C02F3/34C12R1/01C02F101/36
CPCB01D53/72B01D53/84C12N1/20C02F3/34C02F2101/36C12R2001/01C12N1/205Y02A50/20
Inventor 张士汉应赞赞尤菊平陈建孟
Owner ZHEJIANG UNIV OF TECH
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