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A strain of Paleobacter zjutcb-1 that efficiently degrades chlorobenzene and its application

A technology of ZJUTCB-1 and Paleobacterium pallidum, applied in the field of biological treatment of environmental pollutants, can solve the problems of not meeting high-efficiency production, maintaining high efficiency, low degradation rate, etc., and achieve the effect of low purification cost, easy promotion, and low pollution

Active Publication Date: 2022-07-22
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1980s, a large number of scholars at home and abroad have screened out some chlorobenzene-degrading microorganisms from sludge, water, and soil, including Psudomonas sp.strain WR1306, P.putida&Bacillus sp., Escherichiahermanii, Pseudomonas sp.strain JS150, Ralstonia sp. .strain JS705, Aeromonas sp., Pseudomonas sp.strain FY01&FY04, Bacillus sp.Strain FY02&FY03, Pseudononas sp.Nov.LP01, etc., the above microorganisms can degrade the pollutant under aerobic or anaerobic conditions, but not in aerobic conditions at the same time Maintain high efficiency under and anaerobic conditions, and degradation rate is low (under aerobic and anaerobic conditions, the degradation rate of p-chlorobenzene in 18d is respectively 50% and 37.2%), does not meet the requirement of industrial high-efficiency production

Method used

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  • A strain of Paleobacter zjutcb-1 that efficiently degrades chlorobenzene and its application
  • A strain of Paleobacter zjutcb-1 that efficiently degrades chlorobenzene and its application
  • A strain of Paleobacter zjutcb-1 that efficiently degrades chlorobenzene and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Separation, purification, identification and preservation of Ochrobacterum sp. ZJUTCB-1.

[0021] 1. Screening of Ochrobacterum sp. ZJUTCB-1

[0022] Ochrobacterum sp.ZJUTCB-1 is a Gram-negative bacterium domesticated and isolated from the activated sludge of Hangzhou Qige Sewage Treatment Plant. Specific steps are as follows:

[0023]Add 200ml of inorganic salt culture solution to the 340mL serum bottle, pass high-purity nitrogen gas (40mL / min) for 30min to remove the dissolved oxygen in the inorganic salt culture solution and the air at the top of the serum bottle, add 20mL of activated sludge and 987μmol / L The enrichment culture of chlorobenzene was carried out at 30 °C, 160 rpm. When the concentration of chlorobenzene is 50% of the initial concentration, take out 20 mL of the enriched solution and add it to 200 mL of fresh inorganic salt culture solution, then add 987 μmol / L chlorobenzene, 30 ° C, 160 rpm anaerobic cultivation to the concentration of ch...

Embodiment 2

[0035] Example 2: Activation of Ochrobacterum sp.ZJUTCB-1 and degradation of chlorobenzene with different concentrations under aerobic conditions

[0036] Under the conditions of optimal environmental factors (pH=7 of culture medium, culture temperature of 30℃), the degradation performance and bacterial growth of strain ZJUTCB-1 to chlorobenzene with initial concentration of 347.31-2604.80 μmol / L under aerobic conditions were investigated. . The results showed that the strain could completely degrade 347.31-2604.80 μmol / L of chlorobenzene with an average rate of 82.27-174.85 μmol / (L·h). After the chlorobenzene was fully utilized, the quality of the bacteria ZJUTCB-1 also increased significantly. Proceed as follows:

[0037] Prepare an inorganic salt culture solution (same as Example 1), adjust the pH to 7 with sodium hydroxide or dilute hydrochloric acid solution, and distribute it into serum bottles with an average volume of 340 mL, each bottle of 50 mL.

[0038] The strain...

Embodiment 3

[0041] Example 3: Activation of Ochrobacterum sp. ZJUTCB-1 and degradation of chlorobenzene with different concentrations under anaerobic conditions

[0042] Under the conditions of optimal environmental factors (culture pH=7, culture temperature 30℃), the degradation performance and bacterial growth of strain ZJUTCB-1 under anaerobic conditions with initial concentration of 347.31-2604.80 μmol / L chlorobenzene were investigated. . The results showed that the strain could completely degrade 347.31-1736.53 μmol / L chlorobenzene with an average rate of 97.39-210.38 μmol / (L·h). When the concentration of chlorobenzene was as high as 2604.80 μmol / L, the strain could also complete 88.24 μmol / L of chlorobenzene within 32 hours. % removal, the average removal rate was 102.47 μmol / (L·h). After the chlorobenzene is fully utilized, the quality of the bacteria ZJUTCB-1 also increases significantly. The specific implementation steps are as follows:

[0043]The preparation of seed solution ...

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Abstract

The invention discloses a strain of Bacillus pallor ZJUTCB-1 which can efficiently degrade chlorobenzene and an application thereof. The organic pollutants include toluene, p-xylene, chlorobenzene, 1,2-dichlorobenzene or n-hexane. The method is as follows: inoculate Bacillus pallidum ZJUTCB-1 into an inorganic salt culture solution, add organic pollutants, and carry out a degradation reaction under the conditions of 25-35° C., 160 rpm, and pH=6-8 to realize the degradation of organic substances; Bacillus pallidus ZJUTCB‑1 can degrade chlorobenzene and other common organic pollutants in the industry, such as toluene, p-xylene, 1,2‑dichlorobenzene, n-hexane, etc. The Bacillus pallidum ZJUTCB-1 of the invention can realize efficient purification of chlorobenzene in industrial waste water and waste gas, has low pollution, is easy to popularize, and has low purification cost.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and particularly relates to a strain of Ochrobacterum sp. ZJUTCB-1 with efficient chlorobenzene degradation ability and its application in degrading chlorobenzene. Background technique [0002] Chlorobenzene is the simplest chlorinated aromatic hydrocarbon, insoluble in water, stable in physical and chemical properties, and difficult to degrade naturally. It is widely used in industrial and agricultural production, the synthesis of basic organic solvents and organic compounds, and is also used in medicine, dyes, pesticides and engineering plastics. important intermediates in the field. Chlorobenzene can stimulate the skin, conjunctiva and respiratory organs of animals, inhibit the nerve center, and damage the liver and kidneys. Chlorobenzene also has a "three identical" effect. Based on the above hazards, chlorobenzene has been listed as a priority pollu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C02F3/34C12R1/01C02F101/36
CPCB01D53/72B01D53/84C12N1/20C02F3/34C02F2101/36C12R2001/01C12N1/205Y02A50/20
Inventor 张士汉应赞赟尤菊平陈建孟
Owner ZHEJIANG UNIV OF TECH
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