Ochrobactrum and biological agent thereof, preparation method and application

The technology of Bacillus pallidum and biological preparation, which is applied in the field of microorganisms, can solve the problems of cumulative effect, pesticide residues, no product of thiophanate-methyl degrading agent, etc., and achieves the effects of convenient use and low cost.

Active Publication Date: 2016-01-13
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its stable chemical properties, the degradation half-life in the soil is long, and it can remain in the use site for a long time, which is easy to cause cumulative effects. Excessive use often causes pesticide residues in agricultural products
However, there are few research reports on the biodegradation of thiophanate-methyl residues in the academic circles, and there is no thiophanate-methyl degrader product that can be used on a large scale in the market

Method used

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Examples

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Effect test

preparation example Construction

[0030] The preparation method of the biological preparation in which the palebacterium pallidus of the present invention is an active ingredient comprises the steps of test tube seed culture and liquid fermentation culture, specifically comprising:

[0031] A, test tube culture: the intermedium type paleobacterium ( Ochrobactrum intermedium ) TP206 was inoculated on the slant medium of the test tube, and cultured at a constant temperature of 28-31°C for 0.5-1.5 days to obtain the test-tube species;

[0032] B. Liquid fermentation culture: Inoculate the test tube seed into the liquid medium, and culture it on a shaking table at a temperature of 28-31°C for 1-3 days to obtain a biological preparation in which the active ingredient is the target object, Paleobacterium pallidum.

[0033] The composition of the test tube slant medium described in step A is: glucose 1~3g, beef extract 0.5~1.5g, sodium chloride 0.4~0.7g, MgSO 4 ·7H 2 O0.1-0.3g, agar 1.5-2g, the rest is water, pH val...

Embodiment 1

[0057] Paleobacterium intermedius ( Ochrobactrum intermedium ) The TP206 strain was inoculated on the agar slant of the test tube, and the medium formula was: glucose 1g, beef extract 1.0g, sodium chloride 0.5g, MgSO 4 ·7H 2 O 0.2g, agar 1.5g, distilled water 100ml, pH 6.5; 30 ℃ constant temperature culture for 1 day, to obtain test tube species.

[0058] Inoculate the cultured test tube species into 500ml Erlenmeyer flask liquid medium, fill each bottle with 200ml, ferment at 30°C for 2 days, and rotate at 180~200rpm. The medium formula is: glucose 1g, beef extract 0.2g, bean cake powder 1g, NaCl 0.3g, MgSO 4 ·7H 2 O0.2g, distilled water 100ml, pH value 6.5.

[0059] The culture time is 2 days, and the bacterial concentration is ≥25×10 per ml. 8 cfu / mL, a biological agent with the function of reducing thiophanate-methyl was obtained.

Embodiment 2

[0061] Same as Example 1, the difference lies in the formula of liquid medium, its formula is: glucose 2g, beef extract 0.25g, bean cake powder 1.2g, NaCl0.4g, MgSO 7H 2 O0.2g, distilled water 100ml, pH value 7. The concentration of bacteria is to reach the number of live bacteria per ml ≥ 34×10 8 cfu / mL.

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Abstract

The invention discloses ochrobactrum and a biological agent thereof, a preparation method and application. The ochrobactrum is ochrobactrum intermedi. The biological agent with the ochrobactrum as an active ingredient is prepared by conducting test tube culture and liquid fermentation culture on the ochrobactrum intermedi. The preparing method of the biological agent with the ochrobactrum as the active ingredient comprises the steps of test tube culture and liquid fermentation culture. The application refers to the application of the biological agent with the ochrobactrum as the active ingredient in repairing polluted soil with thiophanate methyl residues and in degrading and removing the thiophanate methyl residues in crops. The thiophanate methyl residues degrading bacteria prepared through fermentation of strains TP206 can be widely used for eliminating thiophanate methyl residue pollution in the soil, can also be directly sprayed to the surface of the soil or surfaces of the crops in agricultural production, can effectively degrade and remove the thiophanate methyl residues and are convenient to use and low in cost.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a pale bacillus and its biological preparation, preparation method and application. Background technique [0002] Pesticides The prevention and control of pests and diseases in modern agricultural production is essential, but at the same time, with the extensive use of pesticides, it also causes artificial pollution to the environment, resulting in the generation of pesticide residues in agricultural products, which directly threatens people's health. my country has put forward the "Pesticide Zero Growth Plan", the purpose is to reduce the impact of pesticides on the environment and people. In terms of reducing pesticide residues, in addition to screening high-efficiency and low-residue pesticides, scientific application methods, pesticide substitution and other technologies, biodegradation is also one of the effective technologies. Since the 1960s, American scienti...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 夏振远雷丽萍师君丽吴玉萍莫笑晗
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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