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Immobilization method of salt-tolerant and cold-tolerant compound strain and application of immobilization method

A compound strain, salt-tolerant technology, applied in the field of combined degradation of ammonia nitrogen and petroleum hydrocarbons, can solve problems such as inability to adapt to high salinity environments

Active Publication Date: 2015-06-10
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low-temperature oil-degrading bacteria strains, culture methods, culture media and their applications have been studied (ZL201010239621.7). The oil-degrading bacteria screened out by this method have a higher degradation rate than 40%, but cannot adapt to high salinity environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The present invention specifically degrades the polluted Liaohe Estuary wetland water body, and the water quality is: NH 4 -N concentration up to 35mg / L, NO 2 -N concentration is 12mg / L, NO 3 -The concentration of N is 28mg / L, the concentration of TN is 85mg / L, the concentration of petroleum hydrocarbon is 20mg / L, and the salinity is controlled at 10‰. The shaking treatment is carried out at a temperature of 10°C, including the following steps:

[0033] (1) Preparation of biochar spheres: Weigh 200 g of corn stalks, wash them with deionized water, dry them to constant weight, crush the reed stalks with a plant stalk grinder and pass through a 20-mesh sieve to obtain reed stalk powder. Furnace firing into biochar. The firing conditions of the muffle furnace are: the heating rate is 10°C / min, the temperature is 300°C, and the holding time is 180min; in order to increase the specific surface area of ​​the biochar, that is, to increase the porosity of the biochar and rem...

Embodiment 2

[0041] Sampling a river polluted by ammonia nitrogen and petroleum hydrocarbons, by detecting NH 4 -N concentration up to 5.6mg / L, NO 2 — N concentration is 1.9mg / L, NO 3 — The concentration of N is 3.8mg / L, the concentration of TN is 16.6mg / L, the concentration of petroleum hydrocarbons is 2.1mg / L and the salinity is controlled at 10‰, and the combination of immobilized salt-tolerant and cold-resistant mixed strains is used to degrade ammonia nitrogen and petroleum hydrocarbons. The method is to carry out shaking treatment under the condition of temperature of 15°C, including the following steps:

[0042] (1) Preparation of biochar pellets: Preparation of biochar pellets: Weigh 100 g of reed straw, wash it with deionized water, dry to constant weight, crush the reed straw with a plant straw grinder and pass it through a 20-mesh sieve to obtain Reed straw powder is fired in a muffle furnace to become biochar. The firing conditions of the muffle furnace are: the heating ra...

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Abstract

The invention discloses an immobilization method of a salt-tolerant and cold-tolerant compound strain and an application of the immobilization method. The immobilization method of the salt-tolerant and cold-tolerant compound strain comprises the following steps: firstly screening efficient anaerobic ammonia oxidation bacteria, denitrifying bacteria and petroleum-degrading bacteria, training salt tolerance and cold tolerance to respectively prepare trained Kuenenia. Anammoxidans, Ochrobactrum.spand Flavobacterium mizutaii; putting prepared biological carbon spheres into a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria enrichment solution, culturing films for the biological carbon spheres and subsequently mixing the degrading bacteria enrichment solution with a salt-tolerant, cold-tolerant, efficient and compound degrading bacteria embedding compound solution, finally putting the biological carbon spheres in the mixed solution into a saturated borate solution and a 2%CaCl2 mixed solution one by one, then putting the biological carbon spheres into a refrigerator, crosslinking an washing to obtain salt-tolerant and cold-tolerant compound strain immobilization biological carbon spheres. The salt-tolerant and cold-tolerant compound strain is applied to purification of ammonia nitrogen and water polluted by petroleum hydrocarbon under the condition with low temperature and high salt, so that the strain is high in content of microbial biomass; obviously, the biological carbon spheres are used as carriers for immobilizing the salt-tolerant and cold-tolerant compound strain; the carriers are wide in material resources, simple to manufacture, suitable for industrialization, high in mechanical strength, excellent in treatment effect, low in cost and free of secondary pollution to the environment.

Description

technical field [0001] The invention relates to an immobilization method and application of a salt-resistant and cold-resistant compound bacterial strain, which specifically utilizes the immobilized anaerobic ammonium oxidation, denitrification and petroleum-degrading bacteria mixed in a certain proportion to jointly degrade ammonia nitrogen and petroleum under low-temperature and high-salt conditions hydrocarbon. Background technique [0002] At present, the water bodies of some rivers in our country are seriously polluted by ammonia nitrogen and petroleum hydrocarbons, which make the water bodies stink and destroy habitats. The reason why it is difficult to treat natural water bodies polluted by petroleum hydrocarbons and ammonia nitrogen is that petroleum hydrocarbons inhibit the oxygen enrichment of water bodies, which reduces the activity of aerobic microorganisms or dies. The commonly used methods for ammonia nitrogen and petroleum hydrocarbon pollution include physic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C12N1/20C02F3/34C12R1/01
Inventor 李岿然黄潇党佳佳李辉田伟君赵阳国白洁陈玲
Owner OCEAN UNIV OF CHINA
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