43 results about "Microsporidium species" patented technology

The invention discloses a group of microsporidium molecule universal detection primers and a kit thereof. The universal detection primers include an upstream primer HMG1F and a downstream primer HMG1R; the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.3, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.4. The detection primers target at the gene HMG1 and can be applied to detecting a plurality of microsporidia simultaneously, and the detection results are reliable, easy to operate and high in sensitivity; especially, the universal detection primers have extremely important practical application significance to early detection of various microsporidia in a sample in practical quarantine work.

The invention discloses a method for early detection of grouper intestinal tract microsporidia and application thereof. The method includes the following steps that 1, a fore intestine of grouper is selected, and intestine content is scrapped; 2, genomic DNA of the fore intestine content of grouper is extracted; 3, with the extracted genomic DNA as a template, a specific primer is adopted to conduct PCR amplification; 4, a PCR amplification product in the step 3 is taken and subjected to electrophoretic analysis, a result is judged according to the size of the amplification product, if 450 bp stripes can be amplified through specific amplification, it is proved that the sample is infected by the grouper intestinal tract microsporidia. The problem that diagnosis is difficult as a grouper intestinal tract microsporidia individual is small is solved, programming and standardization of diagnosis are achieved, detection specificity and sensitivity are high, and the method can be applied to diagnosis of grouper intestinal tract microsporidia disease, applied to investigation of the molecular epidemiology and used for assessing whether aquaculture water and bait are suitable for grouper aquaculture or not.

The present invention discloses a nosema bombycis DNA2 gene and application thereof. According to the invention, firstly cloning is carried out to acquire the nosema bombycis DNA2 gene with a full-length sequence shown as SEQ ID NO.1, an ORF rame sequence is shown as SEQ ID NO.2, and an amino acid sequence of encoding protein is shown as SEQ ID NO.3. On the basis, the invention also provides a specific detection primer set for specific detection of nosema bombycis, the specific detection primer set has very good specificity and sensitivity on nosema bombycis, and has very good application prospect in specific detection of nosema bombycis. Moreover, as a newly discovered protein gene, the nosema bombycis DNA2 gene has potential functional application. Discovery of the DNA2 gene provided by the invention has certain significance for theoretical verification and production practice of treatment and detection.

The invention relates to a Chinese herb compound preparation for preventing and controlling the hepatopancreatic necrosis disease in river crab culture, and belongs to the technical field of aquaculture. A preparing method of the Chinese herb compound preparation mainly includes the steps that Lonicera japonica, Rhus chinensis Mill. and Artemisia apiacea are taken and weighed in proportion, water is added for decoction, decoction liquid is evenly mixed with salicylic acid, citric acid and polyhexamethylene biguanidine hydrochloride according to the weight proportion, split charging is conducted, and finally packaging and warehousing are conducted. The Chinese herb plants safe and nontoxic to river crabs are adopted and cooperate with various substances such as organic acid and environment-friendly macromolecular fungicide, and the Chinese herb compound preparation integrates the functions of resisting bacteria and viruses, killing microsporidia, relieving invasion of toxins in a culture environment, preventing the disease and promoting growth, contains definite ingredients, has a wide effect, can be directly splashed, and is convenient to use, high in bioavailability and free of influences of environmental conditions; after the Chinese herb compound preparation is used, the river crab culture ecological environment can be rapidly remediated, heavy metal and pesticide residues in the culture environment can be rapidly reduced, the quantity of microsporidia, bacteria and viruses in the culture environment can be effectively reduced, the incidence probability of the hepatopancreatic necrosis disease of river crabs can be reduced, body tissue can be protected, growth can be promoted, and the river crab culture yield and quality can be improved.

The invention relates to a primer pair for rapidly detecting silkworm microsporidia and a reagent kit and a detection method thereof. The nucleotide sequence of the primer pair is shown as SEQ ID NO.7 and SEQ ID NO.8. By the utilization of the primer pair, different nucleic acid molecular markers are added into the upstream primer and the downstream primer respectively, PCR products of the marked primers can be directly determined through immuno-gold test strips, the operation steps are simplified, convenience is achieved, specificity is good, and sensitivity is high. Compared with a traditional gel electrophoresis method, the sensitiveness is increased by 10 times, and the primer pair can serve as an ideal detection means for silkworm microsporidia in silkworm finished product eggs and has good application prospects.