43 results about "Microsporidium species" patented technology

The invention provides an important protein gene PTP1 relative with growth and infection of microsporidia of silkworms. The gene sequence CDS for coding the pole-tube protein PTP1 for microsporidia of silkworms is got based on the 9-magification gene data of microsporidia of silkworm and by collinear analysis. The pole-tube protein PTP1 got by the invention is of high importance for studying the breeding and infection mechanism of microsporidia, hence can play role in preventing and treating microsporidia in production practice. According RNAi interference, after the pole-tube protein PTP1 being interfered, the microsporidia loses the capability for infecting the host. The pole-tube protein PTP1 and its potential controlling gene of microsporidia will become a new factor for treating microsporidia infection. In addition, by means of transgenosis, the expression of the water-channel protein gene can be enriched, so that the water-channel protein gene can be used to control such destructive insects as locusts, etc.

The invention discloses a direct PCR detection method of Bombyx mori nuclear polyhedrosis pathogeny BmNPV, comprising the following steps of: firstly, treating the blood of infected Bombyx mori by ethanol precipitation to remove a PCR reaction inhibitor; designing the PCR primer of a polyhedrin gene according to the genome analysis of the Bombyx mori nuclear polyhedrosis pathogeny BmNPV; carrying out PCR amplification on the polyhedrin gene, adding an anti-PCR reaction inhibitor BSA (Bull Serum Albumin) to a reaction system and simultaneously carrying out PCR reaction by the primer of a Bombyx mori chondriosome CO I gene to make positive control; respectively getting the blood of normal Bombyx mori and the inflected midguts of the Bombyx mori infected with white muscardine, green muscardine, a bacterial disease and a microsporidia disease and then carrying out PCR reaction specification analysis; and detecting the PCR reaction result by agar gel electrophoresis. The whole detection process has simple operation and can be completed only by 4 hours. The disease can be diagnosed at the early stage of BmNPV infection by adopting the direct PCR detection method to carry out periodical sampling detection so as to adopt a measure in time to prevent the disease from happening.

The invention relates to a micro-microsporidia spore water suspension used for preventing and controlling locusts, and a preparation method thereof. The active component of the locust micro-microsporidia spore water suspension is locust micro-microsporidia spores, and proper amounts of Morwet D425 and xanthan gum are added. The locust micro-microsporidia spore water suspension has the advantages of simple preparation method, good leaf surface adhesiveness, high heat resistance, substantially improved dispersion and uniformity, normal temperature storage resistance, substantial prolongation of the shelf life, good locust killing effect, and cost reduction, is safe to chemical pesticides, and is important for improving the Chinese locust prevention and treatment technology level.

The invention discloses a method for quickly detecting microsporidia spores based on single cell Raman tweezers, belongs to the technical field of pathogenic bacterial detection. The method mainly adopts a pair of laser tweezers and a single cell Raman spectrum technology to judge spores of pathogenic bacteria microsporidia in a host body or the natural environment. The method is easy and convenient to operate, no staining is needed; detection is directly carried out in germfree water; furthermore, whether a sample contains the microsporidia spores or not can be quickly and intuitively judged; therefore, the method is worthy of being popularized and is simple, convenient, efficient and reliable for detection of the microsporidia spores.

The present invention relates to Lymantria xylina cell lines established from pupal tissues of L. xylina Swinhoe, including NTU-LY-1, NTU-LY-2, NTU-LY-3, and NTU-LY-4. These four cell lines were confirmed to be newly established cell lines derived from L. xylina by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and isozyme analysis. The genotypes and characteristics of the abovementioned cell lines are totally different from other insect cell lines. In addition, these four L. xylina cell lines are susceptible to insect baculovirus of L. xylina multiple nucleopolyhedrovirus (LyxyMNPV), LdMNPV-like virus, PenuMNPV, as well as microsporidia of Nosema sp. (isolated from Eurema blanda) and N. bombycis and the like. Therefore the invention can be applied in multiplication of the abovementioned insect-pathogenic microorganisms to produce biopesticides in pest control. In addition, the cell lines can also be used as hosts for the expression vectors of said baculovirus to produce recombinant proteins.

The invention discloses a macrobrachium rosenbergii microsporidia visualized quick detection kit and a detection method thereof. The macrobrachium rosenbergii microsporidia visualized quick detection kit comprises a microsporidia DNA extraction reagent and a reaction reagent, and the reaction reagent contains a nucleic acid primer set used for detecting macrobrachium rosenbergii microsporidia proliferation, wherein a primer group comprises a primer MrMSP-F3, a primer MrMSP-B3, a primer MrMSP-FIP, a primer MrMSP-BIP, a primer MrMSP-LpF and a primer MrMSP-LpB. According to the macrobrachium rosenbergii microsporidia visualized quick detection kit, a set of optimized quick proliferation reaction system is established, so that not only is macrobrachium rosenbergii microsporidia qualitative detection simpler and faster, high in specificity and high in sensitivity, but also the kit can be used for field detection, and has very high scientific study and economic value.

The invention discloses a group of microsporidium molecule universal detection primers and a kit thereof. The universal detection primers include an upstream primer HMG1F and a downstream primer HMG1R; the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.3, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.4. The detection primers target at the gene HMG1 and can be applied to detecting a plurality of microsporidia simultaneously, and the detection results are reliable, easy to operate and high in sensitivity; especially, the universal detection primers have extremely important practical application significance to early detection of various microsporidia in a sample in practical quarantine work.

The invention discloses a method for early detection of grouper intestinal tract microsporidia and application thereof. The method includes the following steps that 1, a fore intestine of grouper is selected, and intestine content is scrapped; 2, genomic DNA of the fore intestine content of grouper is extracted; 3, with the extracted genomic DNA as a template, a specific primer is adopted to conduct PCR amplification; 4, a PCR amplification product in the step 3 is taken and subjected to electrophoretic analysis, a result is judged according to the size of the amplification product, if 450 bp stripes can be amplified through specific amplification, it is proved that the sample is infected by the grouper intestinal tract microsporidia. The problem that diagnosis is difficult as a grouper intestinal tract microsporidia individual is small is solved, programming and standardization of diagnosis are achieved, detection specificity and sensitivity are high, and the method can be applied to diagnosis of grouper intestinal tract microsporidia disease, applied to investigation of the molecular epidemiology and used for assessing whether aquaculture water and bait are suitable for grouper aquaculture or not.

The invention discloses a pharmaceutical composition containing albendazole for resisting microsporidia for silkworms. The pharmaceutical composition is a medicine compound powder prepared from albendazole, enrofloxacin, a filler and an appropriate compound cosolvent. The pharmaceutical composition disclosed by the invention can be applied to prevention and treatment of a pebrine disease and bacteriosis, and comprises two application modes: directly spraying the medicine compound powder on mulberry leaves to feed silkworms, or blending the medicine compound powder with water, preparing a solution, soaking the mulberry leaves and airing to feed the silkworms. The administration time is continuous 12-15 days until mounting and cocooning of silkworm from the fourth larva of silkworm; and the composition is fed once a day except for a moulting stage. The application method disclosed by the invention is convenient, simple and relatively practical. A result proves that a pebrine disease of the silkworms can be effectively prevented and treated.

The present invention discloses a nosema bombycis DNA2 gene and application thereof. According to the invention, firstly cloning is carried out to acquire the nosema bombycis DNA2 gene with a full-length sequence shown as SEQ ID NO.1, an ORF rame sequence is shown as SEQ ID NO.2, and an amino acid sequence of encoding protein is shown as SEQ ID NO.3. On the basis, the invention also provides a specific detection primer set for specific detection of nosema bombycis, the specific detection primer set has very good specificity and sensitivity on nosema bombycis, and has very good application prospect in specific detection of nosema bombycis. Moreover, as a newly discovered protein gene, the nosema bombycis DNA2 gene has potential functional application. Discovery of the DNA2 gene provided by the invention has certain significance for theoretical verification and production practice of treatment and detection.

The invention relates to a Chinese herb compound preparation for preventing and controlling the hepatopancreatic necrosis disease in river crab culture, and belongs to the technical field of aquaculture. A preparing method of the Chinese herb compound preparation mainly includes the steps that Lonicera japonica, Rhus chinensis Mill. and Artemisia apiacea are taken and weighed in proportion, water is added for decoction, decoction liquid is evenly mixed with salicylic acid, citric acid and polyhexamethylene biguanidine hydrochloride according to the weight proportion, split charging is conducted, and finally packaging and warehousing are conducted. The Chinese herb plants safe and nontoxic to river crabs are adopted and cooperate with various substances such as organic acid and environment-friendly macromolecular fungicide, and the Chinese herb compound preparation integrates the functions of resisting bacteria and viruses, killing microsporidia, relieving invasion of toxins in a culture environment, preventing the disease and promoting growth, contains definite ingredients, has a wide effect, can be directly splashed, and is convenient to use, high in bioavailability and free of influences of environmental conditions; after the Chinese herb compound preparation is used, the river crab culture ecological environment can be rapidly remediated, heavy metal and pesticide residues in the culture environment can be rapidly reduced, the quantity of microsporidia, bacteria and viruses in the culture environment can be effectively reduced, the incidence probability of the hepatopancreatic necrosis disease of river crabs can be reduced, body tissue can be protected, growth can be promoted, and the river crab culture yield and quality can be improved.

The invention discloses a silkworm microsporidia HMG1 gene as well as a specific primer set used for rapidly detecting silkworm microsporidia and application thereof. The primer set comprises an upstream primer HMG1-s and a downstream primer HMG1-sR, wherein a nucleotide sequence of the upstream primer is shown in SEQ ID No.5, and a nucleotide sequence of the lower primer is shown in SEQ ID NO.6. A target gene of the detection primer is a high mobility protein gene HMG1 related to sex of the silkworm microsporidia, when the high mobility protein gene HMG1 is taken as the target gene for a silkworm tissue, especially silkworm egg microsporidia molecular detection, the characteristics of reliable detection result, easy operation, strong specificity and high sensitivity can be realized, and the high mobility protein gene HMG1 can be applied to high-sensitivity and rapid silkworm microsporidia molecular detection, especially early detection of the silkworm microsporidia, and has great significance in practical application.

The invention relates to a primer pair for rapidly detecting silkworm microsporidia and a reagent kit and a detection method thereof. The nucleotide sequence of the primer pair is shown as SEQ ID NO.7 and SEQ ID NO.8. By the utilization of the primer pair, different nucleic acid molecular markers are added into the upstream primer and the downstream primer respectively, PCR products of the marked primers can be directly determined through immuno-gold test strips, the operation steps are simplified, convenience is achieved, specificity is good, and sensitivity is high. Compared with a traditional gel electrophoresis method, the sensitiveness is increased by 10 times, and the primer pair can serve as an ideal detection means for silkworm microsporidia in silkworm finished product eggs and has good application prospects.