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Cultivated silkworm microsporidian water channel protein gene

An aquaporin and microsporidian technology, which is applied in genetic engineering, plant genetic improvement, animal/human peptides, etc., can solve the problems reported by research, such as the lack of silkworm microsporidia aquaporin gene, and achieve increased osmotic expansion. pressure effect

Inactive Publication Date: 2008-03-12
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on microsporidin at home and abroad, so far, there is no research report on the aquaporin gene of silkworm microsporidia

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0051] According to the 9-fold genome data of Bombyx mori, the aquaporin gene coding sequence CDS was searched, and the primer design software Primer 5.0 was used to design primers, BamH I restriction site was added to the forward primer, and Sal I was added to the reverse primer Restriction site: primer-F: 5'-CGGGATCCATGGTATCAAGAAATATATTAAA-3'R: 5'-CGGTCGACATAAAGCTTGTACAAAATCGTG-3', the coding sequence of NbAQP gene was amplified from the genome DNA of No. silkworm, and the amplified fragment was cloned into pMD18-T( TaKaRa) vector, and then sequenced (Shanghai Yingjun), so as to obtain the full-length coding sequence of NbAQP, sequence homology analysis was completed with BLAST on the NCBI site, indicating that the NbAQP gene is a new gene.

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PUM

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Abstract

The invention provides a water-channel protein gene relative with sprouting of microsporidia of silkworms. The gene sequence CDS for coding the water-channel protein NbAQP for microsporidia of silkworms is retrieved based on the 9-magification gene data of microsporidia of silkworm, and is proved by a series of biologic cloning technologies. The water-channel protein NbAQP got by the invention is of high importance for studying the sprouting mechanism of microsporidia, hence can play role in preventing and treating microsporidia in production practice. According RNAi interference, after the water-channel protein NbAQP being interfered, the microsporidia loses the capability for infecting the host, this provides a forceful means for preventing and treating granulosis of silkworms; in addition, by means of transgenosis, the expression of the water-channel protein gene can be enriched, so that the water-channel protein gene can be used to control such destructive insects as locusts, etc.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an aquaporin gene with obligate water-passing activity derived from the microsporidia membrane of the silkworm. Background technique [0002] Microsporidia belongs to Protista and Microspora (Levine and Corliss 1980). Recent studies have shown that Microsporidia have a close relationship with fungi in evolution and were formally identified by NCBI For fungi. There are nearly 150 genera and more than 1,200 species of microsporidia. They are spores that specialize in intracellular parasitism, lack mitochondria, and have strong resistance. host tissue. It can infect a wide range of invertebrates and vertebrates, especially economically valuable insects, fish, rodents, lagomorphs, fur animals, primates, and household pets such as dogs and birds, causing severe disease and even death. Among them, Nosema bombycis can parasitize silkworms through feeding and embryonic pathways, causing s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C07K14/37C12N15/30C07K14/44
Inventor 周泽扬潘国庆潘敏慧向仲怀
Owner SOUTHWEST UNIVERSITY
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