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Locust micro-microsporidia spore water suspension and preparation method thereof

A technology for locust microsporidia and microsporidian spores, which is applied in the field of microsporidian spore water suspensions, can solve the problems of poor stability, low suspension rate, easy evaporation, etc., achieve improved dispersion and uniformity, and simple preparation method , to maintain the active effect

Inactive Publication Date: 2016-05-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing dosage forms and application methods often use low-concentration microsporidia locust water, which is mainly made by directly mixing water and microsporidia spores. The suspension rate is low, the stability is poor, and it is easy to evaporate and is not suitable for ultra-low-volume spraying on aircraft. Issues such as Shi are prominent

Method used

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  • Locust micro-microsporidia spore water suspension and preparation method thereof
  • Locust micro-microsporidia spore water suspension and preparation method thereof
  • Locust micro-microsporidia spore water suspension and preparation method thereof

Examples

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Effect test

Embodiment 1

[0027] The ingredients of the processed dosage form are D425 (Morwet polycondensate of alkylnaphthalene sulfonate), xanthan gum, sodium lactate and water. Prepare locust microsporidia spore aqueous suspension according to the following steps:

[0028] 1) Weigh 0.04% xanthan gum according to the proportion of the total amount of preparation, add an appropriate amount of water, and stir for 2 hours until the xanthan gum is completely dissolved;

[0029] 2) Add 0.3% D425 and 0.05% sodium lactate to the xanthan gum solution according to the proportion of the total amount prepared, and mix well to obtain a mixed solution;

[0030] 3) Take out the mother liquid of 10 billion locust microsporidia spores frozen and preserved, thaw, shake well, measure the mother liquid according to the proportion of the total amount of preparation and add it to the mixed solution, then make up water, stir evenly for 2 hours, mix well, and then it can be made 20 million spores / mL Microsporidia locust ...

Embodiment 2

[0032] The ingredients of the processed dosage form are D425 (Morwet polycondensate of alkylnaphthalene sulfonate), xanthan gum, sodium lactate and water. Prepare locust microsporidia spore aqueous suspension according to the following steps:

[0033] 1) Weigh 0.05% xanthan gum according to the proportion of the total amount of preparation, add an appropriate amount of water, and stir for 2 hours until the xanthan gum is completely dissolved;

[0034] 2) Add 0.1% of D425 and 0.03% of sodium lactate to the xanthan gum solution according to the proportion of the total amount of preparation, and mix evenly to obtain a mixed solution;

[0035] 3) Take out the mother liquid of 10 billion locust microsporidia spores frozen and preserved, thaw, shake well, measure the mother liquid according to the proportion of the total amount of preparation and add it to the mixed solution, then make up water, stir evenly for 2 hours, mix well, and then it can be made 10 million spores / mL Microsp...

Embodiment 3

[0037] The ingredients of the processed dosage form are D425 (Morwet polycondensate of alkylnaphthalene sulfonate), xanthan gum, sodium lactate and water. Prepare locust microsporidia spore aqueous suspension according to the following steps:

[0038] 1) Weigh 0.01% xanthan gum according to the proportion of the total amount of preparation, add an appropriate amount of water, and stir for 2 hours until the xanthan gum is completely dissolved;

[0039] 2) Add 0.5% of D425 and 0.01% of sodium lactate to the xanthan gum solution according to the proportion of the total amount of preparation, and mix evenly to obtain a mixed solution;

[0040] 3) Take out the mother liquid of 10 billion locust microsporidia spores frozen and preserved, thaw, shake well, measure the mother liquid according to the proportion of the total amount of preparation and add it to the mixed solution, then make up water, stir evenly for 2 hours, mix well, and then it can be made 50 million spores / mL Microsp...

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Abstract

The invention relates to a micro-microsporidia spore water suspension used for preventing and controlling locusts, and a preparation method thereof. The active component of the locust micro-microsporidia spore water suspension is locust micro-microsporidia spores, and proper amounts of Morwet D425 and xanthan gum are added. The locust micro-microsporidia spore water suspension has the advantages of simple preparation method, good leaf surface adhesiveness, high heat resistance, substantially improved dispersion and uniformity, normal temperature storage resistance, substantial prolongation of the shelf life, good locust killing effect, and cost reduction, is safe to chemical pesticides, and is important for improving the Chinese locust prevention and treatment technology level.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to an aqueous microsporidian spore suspension. Background technique [0002] Locust disasters have always been a natural disaster that seriously threatens my country's agricultural production. There are nearly 1,000 species of locusts recorded in my country, of which more than 60 species can cause harm to agriculture, forestry, and animal husbandry. The current method of controlling locusts is mainly to use chemical pesticides. The advantage of chemical pesticides is that it can quickly reduce the population density of locusts, but its disadvantages are also very obvious, because it often brings pollution to the environment, kills a large number of beneficial animals, and poisons humans and animals. Pests develop resistance and other adverse consequences. [0003] Microbial pesticides to control locusts can not only effectively control locust infestation, but also do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N25/04A01P7/04
CPCA01N25/04A01N63/10
Inventor 张龙
Owner CHINA AGRI UNIV
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