Method for quickly detecting microsporidia spores

Abstract

The invention discloses a method for quickly detecting microsporidia spores based on single cell Raman tweezers, belongs to the technical field of pathogenic bacterial detection. The method mainly adopts a pair of laser tweezers and a single cell Raman spectrum technology to judge spores of pathogenic bacteria microsporidia in a host body or the natural environment. The method is easy and convenient to operate, no staining is needed; detection is directly carried out in germfree water; furthermore, whether a sample contains the microsporidia spores or not can be quickly and intuitively judged; therefore, the method is worthy of being popularized and is simple, convenient, efficient and reliable for detection of the microsporidia spores.

Description

technical field [0001] The invention belongs to the technical field of pathogenic bacteria detection, and in particular relates to a method for directly detecting spores of pathogenic microsporidia based on single-cell Raman spectrum. Background technique [0002] Microsporidia are a group of intracellular obligate parasitic unicellular eukaryotes currently classified as fungi. The life cycle of microsporidia can be divided into three stages: infection period (spore germination), reproduction period (schizogenesis) and sporulation period. Spores are the mature cells of Microsporidia, which spread with the excretion of host feces to infect new hosts, and the germination of spores is the key initiation of Microsporidia infecting hosts. [0003] Microsporidia have a wide range of hosts and can infect invertebrates and vertebrates including humans, causing serious diseases of many insects, aquatic animals and even primates. More than 150 genera and more than 1,400 species have...

AI Technical Summary

Problems solved by technology

Although optical microscopy is relatively reliable and simple, its detection sensitivity is low, and generally it can only be observed in the late stage of infection, and there is a huge lag in detection

Electron microscopic examination must be performed after ultra-thin sectioning, which has relatively high technical requirements for technical equipment and operators. Currently, it is mainly used for scientific research, and it cannot be used for practical diagnosis and identification.

Although the detection method of microsporidia in molecular biology has great advantages over the traditional microscope detection method in terms of sensitivity and specificity, this method requires high experimental skills for operators, and the method used for staining the observation results The dye solution is also highly toxic to the human body

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