34 results about "Hypoxia response" patented technology

The invention belongs to the technical field of nano-pharmaceuticals, and discloses a hypoxia-responsive chiral drug micelle targeting triple-negative breast cancer and a preparation method thereof. The preparation method of the present invention first introduces the azophenyl group into the molecular chain of carboxypolyethylene glycol active ester to obtain carboxypolyethylene glycol azobenzene, and then prepares carboxypolyethylene glycol by reacting it with carboxyl D-polylactic acid. Diol-azobenzene-dextrorotary polylactic acid block copolymer, and then introduce amino-modified nucleic acid aptamer AS1411 to prepare AS1411 modified polyethylene glycol-azobenzene-dextrorotary polylactic acid, a complex fat-soluble anti- After the cancer drug is dialyzed, the micelles of the present invention are obtained. The hypoxia-responsive chiral drug micelles provided by the present invention have small particle size and narrow distribution, and can effectively stay at the target site; have hypoxia responsiveness, and achieve the effect of hypoxia sustained release; can target TNBC tumors, and select Sexual release of anti-cancer drugs, significantly improving the anti-tumor effect.

Compounds and compositions of the following formula:and analogs and stereoisomers thereof (and pharmaceutically acceptable carriers). Additionally disclosed is a method of inhibiting hypoxia-inducible factor-1 function in a patient or sample thereof, comprising administering to the patient or sample an effective inhibiting amount of a compound or composition of the present invention.

The invention discloses a lentiviral expression vector for inducing HPV16 E6 / E7 gene expression under hypoxia conditions. The lentiviral expression vector contains a hypoxia response element, a CMV-minimum promoter and a HPV16 E6 / E7 gene. The lentiviral expression vector can induce directional differentiation of hematopoietic stem cells into immortalized erythroid progenitor cells under the hypoxia conditions and the immortalized erythroid progenitor cells can differentiate into mature erythrocytes under normal oxygen supplying conditions. The erythrocytes induced by the vector has deformability and a oxygen bonding and release capability similar to those of the normal mature erythrocytes and has oxygen content of hemoglobin of 28+ / -2mmHg. The diameter of the erythrocyte injected into a mouse for 10min to 24h is decreased and the erythrocyte has a mature erythrocyte form in 24h and has good adaptability of existence in the body.

The invention relates to a hypoxia response polymer nanoparticle and application thereof. The method comprises the following steps: firstly crosslinking 4,4-diaminoazobenzene and terephthalaldehyde taken as monomers, so as to form a conjugated polymer link, loading a photosensitizer and chemotherapy drugs through noncovalent interaction, and then forming the hypoxia response polymer nanoparticle through a nanoprecipitation method. The hypoxia response polymer nanoparticle can successfully carry the photosensitizer and the chemotherapy drugs to tumor cells, and produce active oxygen during illumination, so as to realize tumor killing. Azo bonds contained in the nanoparticle can be degraded under the action of reductase in cells, and chemotherapy drug release is realized. In addition, cell hypoxia caused during a photodynamic therapy process can further improve drug release, and the hypoxia response polymer nanoparticle can properly realize excellent tumor therapy effect through combination therapy effect of photodynamic therapy and chemical therapy.

The invention discloses a nanoparticle capable of releasing hydrogen sulfide under hypoxia, a preparation method and application thereof. The nanoparticle has uniform size, good stability and good dispersibility in water; the nanoparticle contains water-soluble H 2 S donor, conjugated polymer with hypoxia response, azaboron fluoride complexed dipyrromethine dye with photothermal effect, and coating material, the coating material coats the first three materials to form nanoparticles . The nanoparticles have uniform size, good dispersibility in water, and can release H in an oxygen-deficient environment 2 S, improves the traditional H 2 Problems such as poor solubility and slow release rate of S donor make H 2 S has wider applications in biological diagnosis and treatment and other diseases.