Synthesis method and application of hypoxia response type ratio probe

Abstract

The invention belongs to the field of biological analysis, particularly relates to a synthesis method and application of a hypoxia response type ratio probe, and aims to solve the problem that the hypoxia degree cannot be accurately and reliably evaluated since only qualitative hypoxia detection can be realized in the conventional hypoxia imaging of a living body. The synthesis method comprises the following steps: modifying a fifth generation of polyamide-amine dendrimer PAMAMG5 by adopting an anthocyanin fluorescent dye Cy5-NHS to obtain PG5-Cy5; encapsulating a nitroreductase response type fluorescent molecule Cy7-NO2 in a hydrophobic cavity of PG5-Cy5 to form Cy7-NO2 / PG5-Cy5; and modifying the outer layer of the Cy7-NO2 / PG5-Cy5 with oligo-hyaluronic acid (LWHA) selected as a tumor recognition group, and synthesizing the Cy7-NO2 / PG5-Cy5@LWHA. The hypoxia response type ratio probe prepared by the invention has the characteristics of high sensitivity, high specificity and long in-vivo circulation.

Description

technical field [0001] The invention belongs to the field of biological analysis, and in particular relates to a method for synthesizing a hypoxia-responsive ratio probe and its application. Background technique [0002] A hypoxic microenvironment resulting from rapid tumor cell proliferation and dysmorphic tumor vessels is a ubiquitous feature of solid tumors. In order to adapt to the hypoxic microenvironment, the hypoxia-inducible transcription factor (HIF-1) is activated in tumor cells, which in turn leads to changes in the expression of a series of genes that are associated with metabolic changes, cell migration, metastasis, and therapy resistance. closely related. Therefore, hypoxia is generally considered to be one of the indicators closely related to tumor aggressiveness and poor prognosis. Therefore, quantitative hypoxic imaging is of great significance for understanding the malignant behavior of solid tumors and can help guide treatment and prognosis assessment. ...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned problems in the prior art, that is, the existing living body hypoxia imaging can only realize qualitative hypoxia detection, but cannot accurately and reliably evaluate the degree of hypoxia, the present invention provides a hypoxia The synthesis method of oxygen-responsive ratiometric probes includes: using nanomaterials with internal hydrophobic cavities and rich surface modification sites to modify hypoxia-responsive fluorescent groups, internal reference fluorescent groups and tumor recognition groups to obtain hypoxia-responsive type ratio probe

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