65 results about "Ginkgolide C" patented technology

The invention relates to the field of phytoextraction, in particular to an extraction separation method of ginkgolides. The ginkgolides with fixed components can be obtained through the extraction separation method. The ginkgolides obtained through the steps of extraction, column chromatography, crystallization and crystal mixing in the method comprises bilobalide (C15H1808) of 25.0%-50.0%, ginkgolide A (C20H24O9) of 20.0%-45.0%, ginkgolide B (C20H24O10) of 10.0%-30.0%, ginkgolide C (C20H24O11) of 5.0%-15.0%. The total quantity of the bilobalide, the ginkgolide A, the ginkgolide B and the ginkgolide C amounts for over 95% of the ginkgolides.

The invention provides a gingko-buckthorn tea beverage, which is prepared by lotus leaf, hawkthorn, buckthorn extract, gingko leaf extract, black tea, pu'er tea and water. According to the ginkgolides A (C20H24O9), B (C20H24910) and C (C20H24O11) and bilobalide (C15H18O8), the content of the gingko leaf in the gingko-buckthorn tea is not less than 4.0mg; according to Isorhamnetin (C16H12O7), the content of the buckthorn and the gingko leaf is not less than 0.025mg; according to anhydrous rutin, the content of total flavonoid is not less than 0.13g. The invention also discloses a preparation method for the gingko-buckthorn tea beverage. The gingko-buckthorn tea beverage of the invention has the functions of blood-activating, stasis-dissolving, blood pressure decreasing and blood lipid decreasing, which is applicable for the prevention and health care of middle-aged and aged people, people with high blood lipid and high blood pressure and fat people.

The present invention relates to a new-type high-effective safety medicine-ginkgo leaf total lactone for curing depression. It is a terpene lactone extracted from ginkgo biloba. L or other portions of said plant, such as ginkgo nut, ginkgo root bark and ginkgo twing, in which five terpene lactone compounds of bilobalide A, bilobalide B, bilobalide C, bilobalide J and bilobalide are mainly contained.

The invention relates to a method for preparing ginkgolide compound, belonging to the medicine field. The invention is characterized in that ginkgolide compound raw materials are prepared through the following method that: ginkgo leaves or ginkgo leaf extracts are taken and are extracted in aqueous solution, the obtained extract liquid is filtered and is extracted by using organic solvent, the obtained extract liquid is concentrated and is mixed with 200-mesh to 1,000-mesh silica gel, the obtained mixture is dried, eluent rich in ginkgolide compound is collected by adopting silica column chromatography, the collected eluent is crystallized to respectively obtain ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J, ginkgolide M and ginkgolide K raw materials, and the contents are respectively 90 percent to 110 percent. The invention has the advantages that the purity of the ginkgolide compound prepared through the method is high; and the preparation process is simple, the transfer rate is high, the cost is low and the industrial scale production can be realized.

The invention provides a ginkgolide osmotic pump tablet and a preparation method thereof. The ginkgolide osmotic pump tablet provided by the invention is a double-layer tablet composed of a drug-containing layer and a push layer, and is coated, wherein the drug-containing layer comprises drug release holes. The ginkgolide osmotic pump tablet uses ginkgolide at effective positions of the ginkgo biloba extract as an active component, and a total amount of ginkgolide A, ginkgolide B, ginkgolide Cand bilobalide is not less than 70 %. According to the invention, by combining a solid dispersion solubilising technique and an osmotic pump tablets control-release technology, the ginkgolide is firstly prepared into the solid dispersion, and then further prepared into the ginkgolide osmotic pump tablet on a basis of improving solubility of poorly soluble components, thereby realizing synchronous slow release of complex components in the ginkgolide, reducing drug administration times and improving patient compliance.

The invention provides a novel use of ginkgolide C. Ginkgolide C is a novel and environmentally friendly pesticide having no damage on people and livestock, and has profound meanings to long-term ecological development, human health and mite pest comprehensive control. Compared with a traditional pesticide, in mite pest control, ginkgolide C has the advantages that 1, ginkgolide C is extracted from natural ginkgo leaves, is easy to decompose under the natural conditions, has no damage on the environment and the surrounding ecological system and no pollution on the environment, and is rich in development and application values; 2, ginkgolide C is easy for artificial synthesis and is beneficial for industrialized production; and 3, ginkgolide C can be compounded with other mite pest control active ingredients in preparation-type plants to form a pesticide so that mite pest control effects are further improved.

The invention discloses a ginkgo biloba extract medicinal raw material, and belongs to the field of medicines. The ginkgo biloba extract medicinal raw material is prepared from flavone and lactone which are extracted from ginkgo, wherein the flavone is prepared from quercetin, kaempferol and isorhamnetin; the lactone is prepared from bilobalide, ginkgolide A, ginkgolide B and ginkgolide C; a proportion of the flavone to the lactone is 1 to 1. According to the ginkgo biloba extract medicinal raw material disclosed by the invention, the proportion standard of the flavone and the lactone in original ginkgo biloba extract is changed, and an optimal proportion of a medicine for scavenging free radicals and resisting platelete aggregation is determined.

The invention relates to a high-purity ginkgolide and its composition. It is a white powder with slightly bitter taste. The content of ginkgolide A is 10-20%, the content of ginkgolide B is 10-20%, and the content of ginkgolide C is 10-20%. 5-15%, the bilobalide content is 30-50%, and the total lactone content is 80-95%. Ginkgo biloba is used as raw material, heated and refluxed with ethanol solution with a mass fraction of 20-80% to extract, the extract is concentrated to recover ethanol, separated by a macroporous resin column, desorbed by ethanol, diluted with water, and then passed through a gel-type hydrogen bond Adsorption and desorption of the adsorption resin, collection of effluent and desorption liquid, concentration, drying, ethanol dissolution, activated carbon decolorization, concentration and drying. The preparation process is simple, reliable, low in cost and high in yield, and no toxic organic solvent is used in the process, which is an environment-friendly process. The high-purity ginkgolide is used as an active ingredient to prepare a composition that can be used as various preparations (capsules, tablets, oral liquids, granules, powders, injections, etc.) of ginkgolide medicines or health care products.

The invention discloses a method for extracting and separating ginkgolides C from folium ginkgo, which comprises the following steps: 1) taking the folium ginkgo, carrying out extraction by ethanol with different concentrations from high to low, combining extracting solutions, and reclaiming the ethanol to obtain concentrated extracting solution; 2) adding ethyl acetate into the concentrated extracting solution for extraction, reclaiming organic phase and concentrating the extracting solution into extract; 3) diluting the extract obtained in step 2), putting the extract to preferential polar imperforate adsorption resin, washing the preferential polar imperforate adsorption resin by ethanol, reclaiming the ethanol in eluate, and crystallizing by the ethanol to obtain ginkgolides compounds; 4) putting the ginkgolides compound crystal to silica gel column, washing the silica gel column by miscible liquids of normal hexane and ethyl acetate, and collecting eluate to obtain eluate rich in ginkgolides C; and 5) reclaiming solvent in eluate collected in step 4), and crystallizing the solvent by the ethanol to obtain ginkgolides C monomer. The method has the advantages of simple process, low production cost, high purity of products, and easy industrial production.

The invention discloses a preparation method of a high purity monomer of lactone components of a ginkgo leaf extract. The preparation method comprises the following steps: raw material pretreatment, high speed counter-current extraction, vacuum liquid chromatography separation, high performance liquid chromatography (HPLC) separation, and solvent recrystallization. The obtained high purity monomer comprises ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J, ginkgolide M, and bilobalide. The preparation method is simple. The purity of prepared ginkgolides can reach 99% or above. The separation efficiency is high, the solvent consumption is little, and the preparation method is suitable for industrial production.

The invention relates to the field of plant extraction and in particular relates to a method for detecting bilobalide injection for treating cardiovascular and cerebrovascular diseases and a method for determining content of active ingredients. The content of ginkgo terpene lactone in every 1ml of the determined bilobalide injection is 1-10mg / ml based on the total amount of bilobalide (C15H18O8), ginkgolides A (C20H24O9), ginkgolides B (C20H24O10) and ginkgolides C (C20H24O11), and preferably 4.25-5.75mg.

The invention discloses a preparation method for ginkgolide C. The method is characterized by comprising the step of carrying out column chromatographic separation on a crude extract of ginkgo biloba by using a macroporous reverse-phase adsorption resin, wherein, parameters of the macroporous reverse-phase adsorption resin are that a particle size is 50 to 100 mu m, bore diameter is 90 to 110 A and a resin framework is polystyrene-divinylbenzene. The preparation method provided in the invention enables high product yield to be obtained and product purity to be high.

The subject invention provides ginkgolide C derivatives compounds having the structure: wherein R is H or -A-Ar, where A is an alkyl group; and Ar is an aryl group, which may contain heteroatoms and may be unsubstituted or substituted by one to five substituents each selected from the group consisting of hydrogen, alkoxy, —CH2CO2R4, and —CH2CONR5R6; where R4 is an alkyl group; and R5 and R6 are each, independently, hydrogen or a branched or unbranched alkyl group; wherein R1 is H or —COR7, where R7 is alkyl, aryl or amino; wherein R2 is present or absent, and when present is H, —COR8 or —CO-Z-R8; where R8 is alkyl, aryl or amino; and Z is oxygen; wherein R3 is present or absent, and when present is —COR9; where R9 is alkyl or aryl; wherein only one of R2 or R3 is present in the compound; wherein only two of R, R1, R2 and R3 are H; and wherein each of a and b designates a single covalent bond which is present or absent, where bond a is present when R3 is absent and bond b is present when R2 is absent; or an optically pure enantiomer of the compound. Additionally, the subject invention provides methods of inhibiting the activity of a glycine receptor using these compounds.

The present invention discloses a compound yinlingtong capsule quality detection method, which provides thin-layer identification on ganoderma lucidum, radix scrophulariae and acorus tatarinowii, wherein a high-performance liquid chromatography method is used to carry out content determination on ginkgolide A, ginkgolide B, ginkgolide C and bilobalide. According to the present invention, when the quality control method is used to carry out separation detection, advantages of interference factor resisting, high detection efficiency, good stability, rapid analysis and short detection period can be provided; and the method is the reliable compound yinlingtong capsule control method, and establishes the accurate and feasible control standard for the compound yinlingtong capsule quality detection.

A ginkgolide type compound which serves as a powerful antagonist of platelet activating factors (PAF) has very good protective effects on focal cerebral ischemia and diffuse total cerebral ischemia. Ginkgolide is divided into ginkgolide A, ginkgolide B, ginkgolide C, bilobalide, ginkgolide K, ginkgolide L and the like. The content of ginkgolide L in ginkgo leaves is extremely low, so that ginkgolide L is difficult to obtain, and research report on ginkgolide L is nearly zero. The invention relates to a preparation method of ginkgolide L. Ginkgolide L is extracted from commercially available ginkgo total lactone or ginkgo total lactone obtained based on a known method. The preparation method is simple to operate, the raw materials are low in cost, a plenty of ginkgolide L can be obtained rapidly, and basis is provided for research on ginkgolide L.

The invention provides a method for detecting ginkgolide effective parts, relates to the field of plant extraction, particularly relates to a method for extracting and separating ginkgolide, and based on the extraction and separation method, the ginkgolide with unchanged components is obtained. The ginkgolide with fixed components is obtained by the steps of extraction, leaching, column chromatographic separation, crystallization and crystal mixing and contains 25.0-50.0 percent of bilobalide (C15H18O8), 20.0-45.0 percent of ginkgolide A (C20H24O9), 10.0-30.0 percent of ginkgolide B (C20H24O10) and 5.0-15.0 percent of ginkgolide C (C20H24O11), and the total amount of the bilobalide, the ginkgolide A, the ginkgolide B and the ginkgolide C is more than 95 percent.

This invention provides different compositions extracted from Ginkgo biloba leaves. Said compositions comprise new active components. This invention also provides a method of preparation of the compositions and individual components of said compositions. Finally, this invention provides various uses of this composition.

The invention relates to the field of phytoextraction, in particular to a ginkgolide composition for treating cardiovascular and cerebrovascular diseases. Ginkgolide with fixed components can be acquired by a method for extracting and separating the ginkgolide. The method includes steps of collecting, extracting, passing-through-column treatment, crystallization and crystal mixing to obtain the ginkgolide composition. The ginkgolide composition comprises 25.0-50.0% of bilobalide (C15H18O8), 20.0-45.0% of ginkgolide A (C20H24O9), 10.0-30.0% of ginkgolide B (C20H24O10) and 5.0-15.0% of ginkgolide C (C20H24O11), and the total content of the bilobalide, the ginkgolide A, the ginkgolide B and the ginkgolide C is higher than 95%.

The invention discloses a novel ginkgo dipyridamole composition and a preparation method of a preparation thereof. The ginkgo dipyridamole composition mainly comprises the following ingredients by weight: 25-35% of ginkgo total flavonoid glycol glycosides, 10-15% of dipyridamole and 10-18% of ginkgo total lactones, wherein the ginkgo total lactones are composed of bilobalide, ginkgolides A, ginkgolides B, ginkgolides C and ginkgolides M according to the weight ratio of 22-30: 15-18: 5-8: 8-22: 4-8. The ginkgo dipyridamole composition is high in active ingredient content and low in volume of contained impurities of ginkgo biloba phenolic substances (such as ginkgolic acid), proteins, tannins, pyrogen materials and the like. The pharmaceutic preparation, especially injection prepared by theginkgo dipyridamole composition is suitable for preventing and treating coronary diseases, thromboembolic diseases and the like, has obvious curative effects, and is safe in clinic use.

The invention discloses a ginkgolide sublingual tablet, belonging to the technical field of a pharmaceutical composition, and a preparation method thereof. The sublingual tablet comprises the following materials in parts by weight: 1-200 parts of ginkgolide, 2-20 parts of a disintegrating agent and 40-200 parts of a filler, wherein the ginkgolide includes the following components in percentage by weight: 35-50% of ginkgolide A, 6-16% of ginkgolide B, 15-25% of ginkgolide C and 30-45% of bilobalide. The ginkgolide sublingual tablet disclosed by the invention is prepared by organically combining the disintegrating agent, the filler and the like; the sublingual tablet, which is located under the tongue when being taken, can disintegrate rapidly under the tongue; the sublingual tablet is absorbed through sublingual mucosa and directly participates in blood circulation through jugular vein and superior vena cava to become effective rapidly, so as to relieve migraine rapidly.

The invention provides a ginkgolide C derivative and application thereof to pharmacy. The ginkgolide C derivative has a structural formula shown in the description, wherein R1 represents -X-Ar, -X-Z-Ar, -X-COOH, -CO-Ar, -SO2-Ar, -X-Het., X1-CO-X2-NR4R5 or -X-NR4R5; X, X1 and X2 represent alkylidene groups with 1-8 carbon atoms; Z represents carbon, oxygen, sulfur or nitrogen; and Ar represents pyridyl, naphthyl, pyrimidyl and quinolyl. The ginkgolide C derivative is properly processed and then applied to pharmacy and can be used for greatly improving the bioavailability of an original compound, enhancing the curative effect of a medicine and embodying the pharmaceutical value of ginkgolide C.

The invention discloses a separation and purification method of bilobalide C, which comprises the steps that an extract of ginkgo biloba is taken and extracted by an organic solvent; the extract is treated by MCI-GEL CHP (Cumene Hydroperoxide) adsorbent resin, subjected to impurity removal and eluted; an eluant is subjected to pressure reduction solvent recovery till dryness; 10-90% ethanol is added, heated, dissolved, refrigerated and kept for 12-48h; crystals are separated out, and subjected to suction filtration; crystallization is performed repeatedly; and the crystals are washed by purified water and dried to form the bilobalide C with the purity capable of reaching above 98%. According to the method, a menstruum for separating and purifying the bilobalide C is simple and easy to obtain, and used equipment is conventional equipment, so that the method is simple to operate, less in energy consumption and low in cost; and nontoxic adsorbent absorption and ethanol desorption are adopted, so that the method is safe in production and pollution-free, and is suitable for industrial production.

The invention discloses application of ginkgo leaf total lactones in preparation of a medicament for preventing or treating deafness and tinnitus. The ginkgo leaf total lactones are extracted from ginkgo leaves, the lactone component content is 50 to 99 percent by HPLC (High Performance Liquid Chromatography) detection, and the ginkgo leaf total lactones mainly comprise four chemical components, namely ginkgo lactone A, ginkgo lactone B, ginkgo lactone C and ginkgo seed lactone. The inventor performs experiments of the ginkgo leaf total lactones on a rat audition effect, and the results show that the ginkgo leaf total lactones have certain protection effect on the rat audition, the effect of the ginkgo leaf total lactones is obviously superior to that of ginkgo leaf extract and a quantitative effect relationship is formed. Therefore, the ginkgo leaf total lactones can be used for preparing the medicament for preventing or treating the deafness and the tinnitus. The medicament is a medicinal composition prepared from the ginkgo leaf total lactones and medicinal auxiliary materials according to a conventional method. The medicament can be prepared into oral preparations such as tablets, capsules and the like, can also be prepared into injection preparations such as injection, freeze-dried powder and the like, and can further be prepared into patches and the like.

The invention relates to the field of medicine, in particular to application of one or more ginkgo terpene lactone compounds or pharmacologically acceptable salts, esters, hydrates, solvates and isomers of the ginkgo terpene lactone compounds or any crystal forms, racemic mixtures and metabolites of the ginkgo terpene lactone compounds or a mixture of the ginkgo terpene lactone compounds being used as effective components to preparation of drugs and health care products for preventing and / or treating tremor. The problem of the lack of the drugs for preventing and / or treating the tremor in an existing medicine field is solved. The ginkgo terpene lactone compounds and compositions both obviously improve the state of an illness of an essential tremor model mouse, and the improvement action ofa ginkgo terpene lactone B and bilobalide composition and a bilobalide A, bilobalide B and bilobalide C composition on the essential tremor model mouse is close to that of a positive drug propranololhydrochloride. The state of the illness of essential tremor patients and vascular tremor patients is obviously improved, and no adverse reaction is found in clinical observation cases.

The subject invention provides ginkgolide C derivatives compounds having the structure:wherein R is H or -A-Ar,where A is an alkyl group; andAr is an aryl group, which may contain heteroatoms and may be unsubstituted or substituted by one to five substituents each selected from the group consisting of hydrogen, alkoxy, —CH2CO2R4, and —CH2CONR5R6;where R4 is an alkyl group; andR5 and R6 are each, independently, hydrogen or a branched or unbranched alkyl group;wherein R1 is H or —COR7,where R7 is alkyl, aryl or amino;wherein R2 is present or absent, and when present is H, —COR8 or —CO—Z—R8;where R8 is alkyl, aryl or amino; andZ is oxygen;wherein R3 is present or absent, and when present is —COR9;where R9 is alkyl or aryl;wherein only one of R2 or R3 is present in the compound;wherein only two of R, R1, R2 and R3 are H; andwherein each of a and b designates a single covalent bond which is present or absent,where bond a is present when R3 is absent and bond b is present when R2 is absent;or an optically pure enantiomer of the compound. Additionally, the subject invention provides methods of inhibiting the activity of a glycine receptor using these compounds.

The invention provides a method for preparing bilobalide, bilobalide A, bilobalide B and bilobalide C.The method comprises the steps that ginkgo leaves are treated, and crude total terpene lactones are obtained; the crude total terpene lactones are mixed with silica gel, and a silica gel sample is obtained; the silica gel sample is subjected to gradient eluting in a vacuum liquid chromatogram separating unit, the eluting ratio of a weak polar solvent A to a strong polar solvent B in the gradient eluting process is (8.2-7.8):(1.8-2.2), and bilobalide is obtained; when the eluting ratio is (7.2-6.8):(3.2-2.8), bilobalide C is obtained; when the eluting ratio is (6.2-5.8):(4.2-3.8), bilobalide A is obtained; when the eluting ratio is 1:(0.5-1.5), bilobalide B is obtained.The yield and purity of bilobalide, bilobalide A, bilobalide B and bilobalide C prepared through the method are high.

The invention discloses a medicine composition for treating gastric ulcer. The medicine composition for treating gastric ulcer comprises the following main raw materials in parts by weight: 20-30 parts of astragalus extract, 5-15 parts of radix paeoniae alba extract, 8-12 parts of halcinonide, 8-14 parts of tea polyphenol, 4-6 parts of calcium gluconate, 17-23 parts of formononetin, 8-15 parts of ginkgolides, 2-7 parts of Schisantherin and 3-9 parts of rutin. The medicine has anti-inflammation and stomach restoration capability so as to achieve the complete recovery purpose, can treat both symptoms and roots causes, and is fast in effect, low in toxic and side effects and low in cost.

The invention relates to a content determination method of gingko diterpene lactone for injection, especially a method for simultaneous determination of the content of ginkgolide A, ginkgolide B, ginkgolide C and ginkgolide K in ginkgolide for injection by HPLC-ELSD technique. The method adopts a C18 chromatographic column, takes tetrahydrofuran-water-methanol (n-propanol) as the mobile phase, and determines the lactone content by means of gradient elution. The technology provided by the invention is simple, fast and accurate, and provides certain scientific basis for quality control of the gingko diterpene lactone for injection.

The invention discloses an application of a bilobalide composition to preparation of a medicine for preventing / treating glaucoma. The bilobalide composition consists of total lactone of ginkgo and medicinal auxiliary materials, wherein the total lactone of ginkgo is used as an active component of the bilobalide composition. The total lactone of ginkgo mainly comprises bilobalide A, bilobalide B, bilobalide C and bilobalide, and HPLC-ELSD detection shows that the content of the total lactone is 80.0% to 99.9%. The bilobalide composition disclosed by the invention is subjected to curative effect detection and safety detection and the results show that the bilobalide composition has the effects of reducing intraocular pressure, expanding blood vessels, increasing blood flow volume, improving blood supply of optic nerves and the like, can be used for preparing the medicine for preventing / treating glaucoma diseases, provides a new traditional Chinese medicine for treating the glaucoma diseases in clinical application and has relatively large clinical application value and relatively good social benefits.

The invention relates to a cosmetic product effect additive, and particularly provides an anti-aging ginkgo leaf composition, which contains, by weight, 50-95 parts of ginkgo leaf total lactone, 22-60parts of ginkgo leaf total flavone, and less than 0.0035 part of ginkgo leaf ginkgolic acid, wherein the ginkgo leaf total lactone is one or a plurality of materials selected from ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J and bilobalide, and the ginkgo leaf total flavone is one or a plurality of materials selected from rutin, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside and myricetin. According to the present invention, the composition can be obtained by mixing the monomers, and can further be obtained by extracting, separating and purifying ginkgo leaves; the effective components in the ginkgo leaves can be effectively enriched through the multi-stage purification with different resins so as to significantly improve the cell aging delaying effect of the composition; and the anti-aging ginkgo leaf composition can be widely used in various cosmetics.