50 results about "Eperythrozoonoses" patented technology

The invention discloses swine eperythrozoonosis-resisting compound long-acting oxytetracycline injection, which comprises the following components in part by mass: oxytetracycline hydrochloride (or alkaloids thereof), roxarsone, acetaminophen, glycerol formal, polyvinylpyrrolidone K12, polyvinylpyrrolidone K17, polyethylene glycol 200, polyethylene glycol 400, magnesium chloride, sodium formaldehyde sulphoxylate, monoethanol amine and water for injection. Due to the compatibility of the oxytetracycline hydrochloride (or the alkaloids thereof), the roxarsone and the acetaminophen and synergistic action of each medicament component, the injection treats both symptoms and root causes of swine eperythrozoonosis so as to fulfill the aim of quick and effective treatment.

The invention discloses a compound sulfamonomethoxine / compound sulfamonomethoxine sodium injection which is prepared from main components and auxilary components, wherein the main components comprise 5-20 parts by weight of sulfamonomethoxine / sulfamonomethoxine sodium, 1-4 parts by weight of diclofenac sodium and 1-4 parts by weight of trimethoprim and the auxilary components comprise 0.2-0.4 part by weight of antioxidant, 40-70 parts by weight of organic solvent, 0.2-1 part by weight of regulating reagent and 10-20 parts by weight of water for injection. The injection prepared by the invention can provide effective treatment on secondary infection and mixed infection of streptococcicosis, toxoplasmosis, eperythrozoonosis, colibacillosis, asthma and other diseases, has obvious effect if applied for a long term, strong effect enhanced by several times or dozens of times, hardly generates drug resistance and has the advantages of less consumption, less application times, little irritation and quick treatment effect, can treat both symptoms and root causes, save production cost, improve product additional value and reduce toxic or side effects caused by mismatching of medicaments during treatment.

The invention discloses a compound sulphamethoxazole injection and a preparation method thereof. The injection is prepared from 5-20 % of the main drug sulphamethoxazole, 2-10 % of flunixin meglumine, 1-4 % of trimethoprim, 0.2-0.4 % of an accessory anti-oxidant, 40-70 % of an organic solvent, 0.5-2 % of an adjusting reagent and the balance being water for injection. The injection prepared by the invention can provide effective treatment for secondary infections and mixed infections of streptococcus diseases, toxoplasmosis, eperythrozoonosis, E. coli diseases, asthma and other diseases, is obvious in long-term effects, with a drug effect being enhanced by several times to dozens of times, small in application amount, few in drug using times, little in irritation and fast in performance of drug effects, saves production cost, increases added value of products, and reduces toxic and side effects due to drug mismatch during treatment.

A liquid injection for treating the epierythrocyte disease of domestic animal is prepared from trinitroamidin, doxycycline, the additive chosen from sodium sulfite, sodium hydrogen sulfite, sodium pyrosulfite, etc, and the injection solvent chosen from polyethanediol, glycerine, propanediol, etc through sequentially dissolving in injection solvent while stirring, regulating pH value, filtering, sterilizing, cooling and examining.

The invention provides a construction method of the SPF mouse infection model of an epperythrozoon in the biological technology field, which includes the steps of (1) the separation and purification of the epperythrozoon, (2) the feeding of the SPF mouse in the isolation chamber for the fumigation and the disinfection, (3) the vaccination, (4) the detection evaluation of the animal model. The invention successively constructs the SPF mouse infection model of the epperythrozoon and is verified by three methods, including fresh blood squash detection, PCR detection and transmission electron microscope detection. The model can be applied to the deep researches in the aspects of the etiology, the pathogenic force, the pathology and the immunology of eperythrozoonosis, etc.

The invention relates to a rapid diagnosis test strip for the eperythrozoonosis and comprises a support layer at the bottom, a middle adsorption layer and an external protective layer; wherein, the adsorption layer is fixed on the support layer and a fibrous layer, an adsorption colloidal-gold double anti fibrous layer or a colloidal-gold antigen fibrous layer and a cellulose film and a layer of water absorbent material are sequentially arranged from a test end on the adsorption layer; the cellulose film is provided with detection blots printed by specific purification antigen solutions of the eperythrozoon of human beings or an animal, comparison blots printed by solutions of goat anti-rabbit IgG or rabbit anti-goat IgG or comparison blots printed by IgG solutions of eperythrozoon antigens of rabbit (goat) anti human beings or other animals; the detection blots and the comparison blots are sequentially arranged. The diagnosis strip is higher in specificity and sensitivity detection and can detect 2 nanograms of correspondent antibody protein; the strip is simple and rapid in operation, visual and accurate in result display and lower in diagnosis cost and is particularly suitable for the rapid diagnosis of the eperythrozoonosis of human beings or animals on site.

The present invention discloses a feed for treating Eperythrozoon suis, and a preparation method thereof. The feed comprises the following raw materials: corn, soybean meal, rice bran, peanut meal, dried ginger powder, malt powder, gravy salt, a traditional Chinese medicine additive and a vitamin premix material. According to the present invention, the feed has advantages of significant Eperythrozoon suis treatment effects, healthy qi strengthening, pathogen eliminating, high cure rate, no toxic-side effect, recurrence resistance, no injury, low cost and the like.

The invention discloses a drug combination for curing pig eperythrozoonosis and a preparation method and the application thereof. The invention is made from peganum nigellastrum bge and glycyrrhiza medicine; the preparation method comprises the steps of decocting, removing impurity by clarifier, so as to obtain clarifying, transparent and red-brown liquid. The invention is applied to treatment of pig eperythrozoonosis with distinctive effect, avoiding chemical drug and arsenic preparation used in curing pig eperythrozoonosis, solving the environment pollution problem caused by curing the disease and avoiding the residues problem of toxic arsenic in animal food as well.

An identification method of erythrocyte inclusion includes placing blood on glass slide, dropping normal saline on it and examining it by microscope for viewing whether there is inclusion or not, dropping hydrochloric or acetic acid on blood with said inclusion and viewing whether there is inclusion to move or not after red blood cell is broken, dropping iodine on blood having inclusion and viewing whether inclusion move is stopped or not, confirming that inclusion is existed if it is neither to restart to move nor to be stained by iodine.

The present invention relates to a compound veterinary imidocarb dipropionate preparation applied to eperythrozoonosis and a preparation method thereof. The present invention is made from the main materials of the imidocarb dipropionate, astragalus extract, florfenicol and artesunate, which are confected with auxiliary materials, by the regular methods of weighing, stirring, mixing, checking, measuring, packing, etc. The compound preparation can be medicated by stirring, and the components and the weight percentages of the compound preparation are 5 percent to 15 percent of the imidocarb dipropionate, 2 percent to 5 percent of the astragalus extract, 3 percent to 12 percent of the florfenicol, 5 percent to 15 percent of the artesunate, 25 percent to 65 percent of beta-cyclodextrin and 10 percent to 40 percent of troxerutin. The present invention has the characteristics of low cost, stable storage, assured effect, thoroughly removing eperythrozoon, etc., and is a novel effective preparation for remedying animal eperythrozoonosis.

The invention discloses a pig-sourced eperythrozoon fluorogenic quantitative PCR detection kit which includes: a TaqMan probe which is designed on the basis of an a1 gene sequence, represented as the Seq ID No.1, of the pig eperythrozoon; and a primer pair which is designed on the basis of the gene sequence, represented as the Seq ID No.1, of the pig eperythrozoon. The pig-sourced eperythrozoon fluorogenic quantitative PCR detection kit based on a1 gene is strong in specificity, high in sensitivity and good in repeatability, can quickly and accurately detect whether the eperythrozoon exists in a pig blood sample at a high throughput, can be used in clinical diagnosis, molecular epidemiological investigation and therapeutic effect evaluation of pig eperythrozoon diseases and pig farm cleaning, and has significant meaning on early-stage quick diagnosis, prevention and control and purification of pig eperythrozoon diseases.

The invention discloses a long-acting oxytetracycline injection and a preparation method of the long-acting oxytetracycline injection, and belongs to the technical field of veterinary preparations. Every 1000ml of the injection comprises the following components: 100-240 g of oxytetracycline, 120-140 g of sodium formaldehyde sulfoxylate, 300-400 g of propylene glycol, 80-120 g of magnesium chloride, 10-50 ml of ethanol amine, 50-100 g of pullulan-polyglutamic acid cross-linked polymer, 10-20 ml of honeysuckle extracting solution, 10-20 ml of buckwheat tea extracting solution, and the balance of water for injection. The product quality is stable, the long-acting oxytetracycline injection does not change the color and does not precipitate in the guarantee period, the drug effect lasts for 4 days by injecting once, and the long-acting oxytetracycline injection has better treatment effects on treating doldrums and hyperthermia caused by eperythrozoonosis, swine enzootic pneumonia, mycoplasma and rickettsial diseases of pigs, as well as high fever and systemic diseases.

The invention discloses pig eperythrozoonosis vaccine and a preparation method thereof. The preparation method comprises preparation processes such as cultivation of pig eperythrozoon, inactivation of pig eperythrozoon, emulsification of vaccine adjuvant and the like, thereby obtaining the inactivated pig eperythrozoonosis vaccine. The physicochemical properties such as the stability and the viscosity of the vaccine are detected, the security of the vaccine is detected through mice, piglets are subjected to immunological experiment, immunity detection on specificity immunity and non-specificity immunity is performed, and the detection on the protection function of the inactivated vaccine for animals and the clinical effects of the vaccine is performed. The vaccine has the advantages that the clinical and experiment data show that the vaccine has the security and the protection capability, further has the characteristics of simple preparation process, easiness to obtain, low cost and the like, and can be applied to clinical veterinarian in large scale. The vaccine is significant for prevention on pig eperythrozoon, and has important clinical meaning in reducing the pig industry loss caused by the pig eperythrozoonosis.

The invention relates to a complex propolis composition for treating eperythrozoon suis and a preparation method thereof, and relates to the animal medicine field. The preparation method comprises the following steps that (1) each ingredient in the prescription is respectively micro-smashed to pass through a 700-mesh sieve; (2) 15 to 30 parts of propolis, 15 to 35 parts of rhizoma coptidis, 15 to 45 parts of Chinese bulbul and 15 to 35 parts of rhubarb are measured by weight and are blended for 5 to 10 minutes until being uniformly blended; (3) 15 to 45 parts of kuh-seng, 15 to 35 parts of cortex meliae, 15 to 40 parts of radix codonopsitis, 15 to 45 parts of radix dichroae and 15 to 45 parts of southernwood are measured by weight and are blended for 5 to 10 minutes until being uniformly blended; and (4) compositions respectively prepared in steps (2) and (3) are mixed to be continuously blended for 10 to 15 minutes until being uniformly blended. The complex propolis composition has beneficial effects that the prescription is reasonable, simplicity in preparation is realized, and the composition is toxic-free, has no side effect and is free from pollution, medicinal residue and drug resistance. Being micro-smashed, the biological availability is high, the curative effect is very remarkable, and a specially good effect on treating eperythrozoon suis is clinically proved.

The present invention discloses a veterinary levamisole hydrochloride compound preparation used for the eperythrozoonosis in the biotechnical field and a preparation method thereof. The present invention considers the levamisole hydrochloride, imidocarb dipropionate and artemether as main materials to be matched with magnesium dichloride, 1, 2-propanediol, refined soybean oil, lecithin and glycerin to be processed by the methods of weighing, crushing, stirring, mixing, checking, measuring, packing, etc. Components and weight percentages are: 3 percent to 12 percent of the levamisole hydrochloride, 5 percent to 15 percent of the imidocarb dipropionate, 2 percent to 10 percent of the artemether, 0.5 percent to 5 percent of the magnesium dichloride, 1 percent to 10 percent of the 1, 2-propanediol, 5 percent to 15 percent of the refined soybean oil, 0.5 percent to 1 percent of the lecithin and 2 percent to 5 percent of the glycerin. The compound preparation of the present invention can be medicated by intramuscular injection and subcutaneous injection, which has the characteristics of low cost, stable storage, assured effect and eliminating eperythrozoon thoroughly.

The invention discloses a polymerase chain reaction (PCR) detection method of porcine eperythrozoon, and the method successively comprises the steps of pathogen separation and purification, porcine eperythrozoon DNA extraction, primer design and synthesis, ordinary PCR detection, positive plasmid standard substance preparation and real-time fluorescent quantitative PCR detection. Compared with IHA (indirect hemagglutination assay), ELISA (enzyme-linked immuno sorbent assay) and other detection methods, the method has the advantages of high specificity, high sensitivity, high accuracy and reliability, can fast, simply, reliably and accurately detect eperythrozoon in a sample, and can also be used for epidemiological investigation and curative effect detection of eperythrozoonosis.

The invention discloses a method for indirect immunofluorescence assay (IFA) detection of eperythrozoonosis by use of a monoclonal antibody. The method includes establishment of a standard positive control and a standard negative control, antigen coating, fluorescence second antibody dilution, IFA determination and other steps. An antibody used in the method is a monoclonal antibody against porcine eperythrozoon, and compared with general antibodies used in IHA (indirect hemagglutination assay), ELISA (enzyme-linked immuno sorbent assay) and other detection methods, the antibody used in the method has the advantages of high specificity, high sensitivity, high accuracy and reliability, can fast, simply, reliably and accurately detect eperythrozoon in a sample, and can also be used for epidemiological investigation and curative effect detection of the eperythrozoonosis.

The invention relates to a veterinary compound clove liniment treating eperythrozoonosis in the biotechnology field and a preparation method thereof. The invention is mainly prepared by main materials, such as clove oils, artemethe and levamisole hydrochloride, served with auxiliary materials, such as propanediol, isopropanol, laurocapram and glycerol, and the main materials and the auxiliary materials are treated by conventional methods, such as weighing, stirring, well mixing, measuring and packaging, etc. The compound preparation can be applied to skins and the weight percentage of raw materials is clove oils of 10 to 20 percent, the artemether of 10 to 15 percent, the levamisole hydrochloride of 5 to 15 percent, the propanediol of 10 to 30 percent, the isopropanol of 20 to 30 percent, the laurocapram of 1 to 5 percent and the glycerol of 2 to 6 percent. The invention has the advantages of a low cost, storage stability, reliable effect and convenient medication, etc., so the invention is a novel and highly-efficient preparation for treating eperythrozoonosis of animals.

The invention relates to a traditional Chinese medicinal composition for preventing and treating swine eperythrozoonosis and a preparation method of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition for preventing and treating the swine eperythrozoonosis is prepared by carrying out scientific compatibility on eleven traditional Chinese medicines, namely sweet wormwood herb, antifebrile dichroa, houttuynia cordata, sophora flavescens, astragalus, ligusticum wallichii, radix paeoniae rubra, liquorice, mirabilite, burdock and cyrtomium rhizome, grinding the traditional Chinese medicines by virtue of an ultrafine grinder and uniformly mixing. The traditional Chinese medicinal composition for preventing and treating the swine eperythrozoonosis is obvious in curative effect, has dual function in prevention and treatment and has a good application practice in the aspect of preventing and treating the swine eperythrozoonosis; meanwhile, the technology is simple, the application is convenient, the cost is low, no toxic or side effect such as teratogenesis and mutagenesis is produced, safety in application is guaranteed, and no residue is produced.

The invention relates to a traditional Chinese medicine composition for treating mycoplasma suis. The traditional Chinese medicine composition is prepared from the following mixed components in parts by weight: 15-35 parts of orange peel, 15-35 parts of honeysuckle stem, 15-45 parts of suberect spatholobus stem, 15-45 parts of cockscomb, 15-45 parts of dalbergia wood, 15-35 parts of galangal, 15-45 parts of adder-wort, 15-45 parts of golden cypress, 15-45 parts of licorice and 15-45 parts of sealwort. The traditional Chinese medicine composition has the beneficial effects that the components are reasonable, the preparation method is simple, the composition does not have toxic and side effects, pollution, drug residue and drug resistance, is in superfine grinding, high in bioavailability and very obvious in curative effect and is clinically proved to have a special effect for treating mycoplasma suis.

The invention relates to a medicinal composition and an injection for treating swine eperythrozoonosis, and preparation methods thereof. The medicinal composition comprises imidocarb hydrochloride, doxycycline hydrochloride, a non-steroidal anti-inflammatory medicament, and glucocorticoid and salt thereof. The injection consists of the following components in percentage by weight: 5 to 15 percent of imidocarb hydrochloride, 5 to 10 percent of doxycycline hydrochloride, 2 to 20 percent of non-steroidal anti-inflammatory medicament, 0.05 to 0.5 percent of glucocorticoid and salt thereof, 0.5 to 5 percent of magnesium chloride, 0.1 to 0.2 percent of antioxidant, 0.01 to 0.05 percent of metal complexing agent, 5 to 20 percent of propylene glycol, and the balance of water for injection. The medicinal composition and the injection have the characteristics of reasonable prescription formulation, stable medicinal physical and chemical properties, accurate dose, quick response, long medicinal effect maintaining time, high bioavailability, safe use and exact curative effect.

The invention discloses a swine-derived eperythrozoon detecion kit, a method and applications. The kit comprises a TaqMan probe and a primer pair designed aimed at a swine-derived eperythrozoon G1 gene. The probe binds to the 658th-793th nucleotide sequence of the swine-derived eperythrozoon G1 gene specifically. The primer pair is used for amplification of the 658th-793th nucleotide sequence of the swine-derived eperythrozoon G1 gene. The swine-derived eperythrozoon real-time fluorescence quantification PCR detection kit based on the attachment protein G1 gene has characteristics of strong specificity, high sensitivity and good repeatability, can detect whether eperythrozoon is existed in a swine blood sample rapidly, accurately and with high throughput, can be applied at aspects of clinical diagnosis, molecular epidemiological investigation, therapeutic effect evaluation, pig farm purification of swine eperythrozoonosis and the like, and has an important meaning to early state rapid diagnosis, prevention and control and purification of swine eperythrozoonosis.

The invention belongs to the field of veterinary drugs, and specifically relates to a compound artemisinin multiphase lipidosome injection as well as a preparation method thereof. The injection comprises a medicinal component and an auxiliary material of the injection, wherein the medicinal component is prepared from artemisinin and diminazene aceturate, and the auxiliary material of the injection consists of lecithin, cholesterol, tween-80, a phosphate buffer and absolute ethyl alcohol. The injection comprises the following components in parts by weight: 1-30 parts of lecithin, 0.5-10 parts of cholesterol, 0.1-5 parts of tween-80, 0.5-5 parts of artemisinin, 0.5-5 parts of diminazene aceturate, 1-15 parts of absolute ethyl alcohol and 5-90 parts of the phosphate buffer. The compound artemisinin multiphase lipidosome injection prepared by the preparation method disclosed by the invention is remarkable in curative effect in treating eperythrozoonosis of livestock and poultry in clinical treatment of veterinarians, and is high in safety. Several methods are organically combined, i.e., an ethanol injection-ultrasonic treatment-homogenization method is adopted. The compound artemisinin multiphase lipidosome injection prepared by the method is high in encapsulation efficiency, good in stability, strong in operability and applicable to industrialized production on a large scale.

The invention discloses a herbal medicine formula for treating mycoplasma suis. Rhizoma smilacis glabrae is sweet, gentle and smooth in property, can act on livers and stomachs, has the functions of dehumidification, detoxifying and joint movement easing, and can mainly treat dampness, hotness, stranguria with turbid discharge, leucorrhea, carbuncle swelling, scrofula, mange, and limb contracture, physique contracture pain, scrofula, swelling and ulcer on the body surface caused by syphilis. The herbal medicine formula is mainly prepared by frying 60g of rhizoma smilacis glabrae, 60g of pokeberry root, 60g of safflower and 20g of ephedra in gentle fire till being slight brown, pouring 1,000ml of rice wine into the hot components, and bottling, sealing up and drinking after three days. The medicine wine is taken according to the proportion of 10-20ml per kilometer of the body weight, heated to be about 40 DEG C and taken in empty stomach, no food or water can be taken within 2 hours after taking the medicine, rain is prevented and wind is prevented as much as possible. The medicine is taken twice a day in the morning and at night for 3 days continuously.

The invention discloses a real-time fluorescent PCR (polymerase chain reaction) kit for detecting swine Eperythrozoon in umbilical cord blood and application thereof; the kit is a real-time fluorescent PCR kit developed according to swine Eperythrozoon membrane protein OxaA gene for the first time and comprises Taqman fluorescent probe and primer designed for swine Eperythrozoon OxaA gene sequences; sequences of the primer are shown as in SEQ ID NO: 1 and SEQ ID NO:2, and a sequence of the fluorescent probe is shown as in SEQ ID NO: 3. The real-time fluorescent PCR kit has the advantages of high specificity, high sensitivity, high accuracy and good repeatability, and is suitable for high-throughput sample detection. The kit of the invention is suitable for detecting Eperythrozoonosis of swine umbilical cord blood, and is also suitable for detecting conventional blood and tissue samples, guiding the prevention of such disease, providing molecular epidemiological investigation and assessing therapeutic effect; in addition, through umbilical cord blood it is also possible to monitor female piglets at the same time, and the kit is important to the early quick diagnosis and preventive control of Eperythrozoonosis.

The invention discloses a kit for indentifying the types of porcine derived eperythrozoons. The kit comprises a primer pair for amplifying a porcine derived eperythrozoon 16S rRNA gene sequence shown by SEQ ID NO:1 or SEQ ID NO:2 and restriction endonuclease Sca I or Ssp I. The kit for identifying the types of the porcine derived eperythrozoons on the basis of the 16S rRNA gene can be used for correctly and quickly identifying two types of eperythrozoons infecting pigs, including a mycoplasma suis and an M.parvum, is beneficial for the works of clinical diagnosis of porcine derived eperythrozoonosis, molecular epidemiology survey and pig farm purification, and has an important significance for early rapid diagnosis, prevention, control and purification of the porcine derived eperythrozoonosis.

The invention belongs to the field of veterinary medicines and particularly discloses a medicine for treating animal eperythrozoonosis. The medicine comprises the following effective components in parts by weight: 0.25-2.5 parts of artesunate, 0.1-1 part of arteether and 0.25-2.5 parts of artemether. The medicine disclosed by the invention belongs to a natural medicine, has the advantages of being free from pollution, low in toxic and side effects, less in residual quantity of poisons and the like, has a good treatment effect on the eperythrozoonosis and can avoid the use of antibiotics.

The invention relates to a traditional Chinese medicine composition for treating porcine eperythrozoonosis. The traditional Chinese medicine composition is prepared by mixing the following components in parts by weight: 15-35 parts of folium artemisiae argyi, 15-35 parts of pyrrosia lingua, 15-45 parts of radix gentianae, 15-55 parts of ginger, 15-65 parts of radix paeoniae alba, 15-55 parts of radix ampelopsis, 15-75 parts of geranium wilfordii, 15-75 parts of hypericum japonicum, 15-45 parts of liquorice and 15-45 parts of fructus kochiae. The traditional Chinese medicine composition has the beneficial effects that the traditional Chinese medicine composition is reasonable in formula, simple to prepare, free of toxic and side effects, free of pollution, free of medicine residues, free of medicine resistance, high in bioavailability and very obvious in curative effect, the traditional Chinese medicine composition can be super finely ground, and clinically-proven results show that the traditional Chinese medicine composition has the special treatment effect on porcine eperythrozoonosis.

A pharmaceutical composition for treating porcine eperythrozoonosis, an injection and a preparation method thereof. The pharmaceutical composition comprises imidazuron hydrochloride, doxycycline hydrochloride, non-steroidal anti-inflammatory drugs, glucocorticoids and salts thereof. The injection consists of the following components by weight percentage: 5-15% of imidazolium hydrochloride, 5-10% of doxycycline hydrochloride, 2-20% of non-steroidal anti-inflammatory drugs, 0.05% of glucocorticoids and their salts ~0.5%, magnesium chloride 0.5~5%, antioxidant 0.1~0.2%, metal complexing agent 0.01~0.05%, propylene glycol 5~20%, and the balance is water for injection. The product of the invention is characterized in that the composition of the prescription is reasonable, the physical and chemical properties of the medicine are stable, the dosage is accurate, the onset of action is fast, the maintenance time of the medicine effect is long, the bioavailability is high, the use is safe, and the curative effect is definite.

Disophenol is an anthelmintic veterinary medicine and mainly used for treating trematodiasis and gastrointestinal nematodosis of various animals. A chemical name of the disophenol is 2,6-diiodo-4-nitrophenol, and a molecular formula of the disophenol is C6H3I2NO3. By application of the disophenol to preparation of medicines for treating animal eperythrozoonosis, animal eperythrozoon can be strongly inhibited and killed, and extraordinary effects are achieved.