44 results about "Eisosome assembly" patented technology

The present invention provides assemblies of oligomeric amyloid beta protein and uses thereof.

The invention discloses a synthesis method of low-polyethylene-glycol functional amphiphilic pillar [5] arene compound (AP5-glycol), wherein AP5-glycol is self-assembled in water to form a vesicle, and the vesicle can generate responsive depolymerization (the vesicle becomes small or gradually disappears) after being affected by external physical stimulation such as heating, ultrasonic treatment, violent stirring and the like and can be rapidly formed again after the external stimulation is stopped, i.e., the two processes including vesicle formation and vesicle depolymerization are reversible and controllable. Therefore, the vesicle has excellent thermodynamic reversibility and controllability. In addition, KPF6 and benzo-18-crown-6 can be respectively used as a switch for depolymerizing and forming a vesicle self-assembly again. The characteristics can ensure that the vesicle has outstanding recyclability in application such as drug release or transfer and the like.

The invention discloses an expression vector of a nuclease protein Cas9 and a construction method and expression purification of the expression vector of the nuclease protein Cas9. According to the expression vector of the nuclease protein Cas9, an original thioredoxin sequence in pET32a is replaced with cDNA of an amplified thioredoxin-histidine-tag-tobacco-etch-virus-protease recognition site, and codons are optimized to a Cas9 gene sequence which is easy to express in a host and has nuclear localization signal peptide and 3xFLAG tag sequence fused in a C-terminal to be integrated into the thioredox in sequence. According to the expression vector of the nuclease protein Cas9 and the construction method and expression purification of the expression vector of the nuclease protein Cas9, codon optimization and thioredoxin fusion expression are used for obtaining a large amount of the nuclease Cas9 protein, under the condition that only an LB medium is used, 10 mg of a highly purified active Cas9 protein can be finally obtained per gram of escherichia coli Tuner (DE3) wet bacteria, and high expression of the high-fidelity nuclease protein Cas9 at a low cost is realized; and the requirements on the host are not high, and the obtained protein can be used for large-scale biological in vitro assembly and in vivo gene editing experiments.

The invention discloses a fluorescent probe based on polyoxometallate and an assembly thereof and an application of the fluorescent probe in spermine detection, and belongs to the technical field of fluorescent probes. According to the invention, a supramolecular assembly strategy is utilized, environment-sensitive polyoxometallate EuW10 is taken as a fluorophore, a peptide fragment GL-22 of HPV E6 is introduced to construct an assembly, and specific recognition and detection of spermine (Spm) are realized. The EuW10/GL-22 assembly can be used for effectively distinguishing spermine (Spm) and spermidine (Spd), so that the specific response to the Spm is realized. The linear response range of the EuW10/GL-22 assembly to spermine is 0.05 to 0.6 [mu] mol/L, and the detection limit is 2.2 nmol/L. The method is quick in response, high in sensitivity and strong in specificity in the aspect of detecting trace spermine biomarkers. Therefore, the detection performance of the probe can be effectively improved through a supramolecular assembly strategy, and high-sensitivity detection of the biogenic polyamine is realized.

The invention relates to an SERS biosensor; hairpin probe DNA is modified on the surface of Fe3O4; MCH is added for incubation to prevent non-specific binding; miRNA, auxiliary chain DA and auxiliary chain DB are added to generate an enzyme digestion product; hairpin DNA 1 and hairpin DNA 2 are modified on the surface of AuNP in advance; the dispersed AuNP modified by the hairpin DNA 1 and the hairpin DNA 2 is added into the incubated Fe3O4 to carry out a hybrid chain amplification reaction; reaction is carried out overnight; and centrifugal washing is carried out. According to the invention, in a nano-assembly SERS detection system, for unreacted gold nano-cluster elements and aggregates generated by non-triggering, Fe3O4 is adopted as a magnetic separation substrate; an assembly triggered by hybrid chain amplification is rapidly separated out through surface functionalization and exclusion of non-specific adsorption, so that the aggregates only containing gold nanoparticles on the Fe3O4 surface are obtained; a very high SERS signal is achieved; and myocardial infarction miRNA detection is achieved.

Disclosed herein are nanostructures and their use, where the nanostructures include a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides selected from 153_dn5A, 153_dn5A.1 and I53_dn5A.2, or variants thereof; and a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides being 153 dn5B or a variant thereof, wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure; and wherein the nanostructure displays multiple copies of one or more paramyxovirus and/or pneumovirus F proteins, or antigenic fragments thereof.

This teaching tool can include: a base having an A base opening, a B base opening, and a Rh base opening; a set of antibody assemblies including; a blood cell model having an set of openings for receiving at least one of a set of antigen assemblies wherein when the A antigen head of the blood cell antigen assembly is received into the A assembly, A agglutinate is represented, wherein the B antigen head of the blood cell antigen assembly is received into the B assembly, B agglutinate is represented, and wherein the Rh antigen head of the blood cell antigen assembly is received into the Rh assembly, Rh agglutinate is represented; and, a capillary representation defining a capillary cavity for comparison with A, B, or Rh agglutinated representations to determine if the A, B or Rh agglutinated can be received in the capillary representation.

According to a fusion design method disclosed by the invention, an alpha helical structure is utilized to fuse a tubulin alpha subunit of Proteobacter dejogii with a D structural domain of a protein Aby a same amino acid substitution method; fusion protein with high feasibility is screened out through alpha helix formation conditions, secondary structure analysis, energy calculation and kinetic simulation; experiment results prove that the screened fusion protein really has microtubule assembly characteristics and the activity of a D structural domain affinity VHIII single-chain antibody of protein A; and on the basis of a naturally existing self-assembly system, namely a microtubule site, other active protein subunits are fused on the microtubulin subunits, so that the activity of combining the microtubule self-assembly system with a single-chain antibody is improved under the condition of keeping the self-assembly activity of the microtubule subunits.