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Biosensor as well as preparation method and application

A biosensor and reaction technology, applied in the field of biosensing, can solve the problems of small difference in color, photobleaching signal-to-noise ratio of biosensors, etc.

Active Publication Date: 2020-10-23
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: In view of the problems that the biosensor constructed by fluorescent molecules in the prior art is prone to photobleaching and has a low signal-to-noise ratio, and the slight difference in color is difficult to be recognized by the naked eye, the present invention provides a biosensor, which is based on Gold-silver core-shell-tetrahedral DNA assembly containing azobenzene molecules for rapid and highly sensitive detection of miR-21

Method used

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  • Biosensor as well as preparation method and application
  • Biosensor as well as preparation method and application
  • Biosensor as well as preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Prepare the gold-silver core-shell-tetrahedral structure DNA assembly biosensor containing azobenzene molecules according to the following steps:

[0048] (1) Preparation of gold-silver core-shell nanoparticles

[0049] Take sodium borohydride reduction to prepare 3nm gold seeds, use the seed growth method to grow the 3nm gold seeds into 30nm gold balls, add 0.01M silver nitrate solution drop by drop, the measured ultraviolet spectrum is 505nm, and the gold and silver nuclei with a size of 50nm are obtained Shell nanoparticles (Au@AgNCs).

[0050] The specific process is as follows:

[0051] a. Preparation of about 3nm GNPs

[0052] Select a clean and treated 20mL glass bottle as the reaction bottle. Put the cleaned magnets into the reaction bottle, and ensure that the entire reaction device is in the middle of stirring. Then add 0.1M CTAB (aqueous, aqueous: aq) 10mL and 0.01M HAuCl to the reaction flask 4 (aq) 0.25mL, during the process of sample addition, stir wi...

Embodiment 2

[0071] The preparation method of present embodiment is identical with embodiment 1, and difference is:

[0072] In step (3), the tetrahedral DNA was diluted at a ratio of 1:100000, and 200 μL of tetrahedral DNA with a concentration of 10 pM was dropped onto the gold-silver core-shell nanoparticles immobilized on the surface of ITO.

Embodiment 3

[0074] The preparation method of present embodiment is identical with embodiment 1, and difference is:

[0075] In step (3), the tetrahedral DNA was diluted at a ratio of 1:10000, and 200 μL of tetrahedral DNA with a concentration of 100 pM was dropped onto the gold-silver core-shell nanoparticles immobilized on the surface of ITO.

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Abstract

The invention discloses a biosensor as well as a preparation method and application thereof. The biosensor disclosed by the invention is an assembly comprising gold and silver core-shell nanoparticle-tetrahedral structure DNA containing azobenzene molecules. The preparation method of the biosensor comprises the following steps of: preparing the gold and silver core-shell nanoparticles; preparing tetrahedral structure DNA containing the azobenzene molecules; and (3) assembling the gold and silver core-shell nanoparticle-tetrahedron DNA assembly containing the azobenzene molecules. According tothe biosensor which is the assembly comprising the gold and silver core-shell nanoparticle-tetrahedral structure DNA containing azobenzene molecules, high-sensitivity detection of miR-21 can be achieved on the single-nanoparticle scale, the concentration of miR-21 and the red shift amount of a scattering spectrum of the gold-silver core-shell nanoparticle-tetrahedral structure DNA assembly containing the azobenzene molecules are in a linear relation, and important significance is achieved for diagnosis and prognosis treatment of early-stage cancer.

Description

technical field [0001] The invention relates to the field of biosensing, in particular to a biosensor for detecting miR-21 by constructing a tetrahedral DNA plasma assembly based on gold-silver core-shell nanoparticles, a preparation method and a use method. Background technique [0002] Regarding the diagnosis of tumors, the detection sensitivity of its biomarkers (such as: DNA, RNA or protein) is much higher than that of traditional analysis methods. MicroRNAs are a class of endogenous non-protein-coding RNAs with a length of 20-24 nucleotides. Usually in early development, cell proliferation, differentiation, apoptosis and other biological processes play a very important role. Abnormal expression of MicroRNAs occurs in precancerous malignant tumor cells and is associated with many cancers (such as: lung, liver cells, large intestine and breast cancer, etc.). MicroRNA-21 (miR-21) exists in many human cancer tissues mentioned above, especially the expression level in lung...

Claims

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Application Information

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IPC IPC(8): G01N21/552G01N21/49G01N21/39G01N1/28
CPCG01N21/554G01N21/49G01N21/39G01N1/28
Inventor 张磊陈雨王露露沈晶晶范曲立黄维
Owner NANJING UNIV OF POSTS & TELECOMM
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