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Terahertz metamaterial chip for cell invasion and migration unmarked detection

A metamaterial and terahertz technology, applied in the field of terahertz measurement, can solve the problems of difficult to evaluate and measure the migration and invasion of living cells, and achieve the effect of facilitating cell chemotaxis, material exchange, and convenient assembly.

Pending Publication Date: 2019-03-12
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing transmissive terahertz metamaterial cell detection chip often needs to wipe off the extracellular water of the cells, and relies on growing a single cell layer on the surface of the metamaterial, so it can only detect the response of static adherent cells to drug-induced apoptosis. , it is difficult to evaluate and measure the movement behavior of living cells such as migration and invasion

Method used

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  • Terahertz metamaterial chip for cell invasion and migration unmarked detection
  • Terahertz metamaterial chip for cell invasion and migration unmarked detection
  • Terahertz metamaterial chip for cell invasion and migration unmarked detection

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Experimental program
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Embodiment

[0050] Such as figure 1 and Figure 2-a , Figure 2-b As shown, the terahertz metamaterial chip used for label-free detection of cell invasion and migration ability includes a cell culture chamber 1 and a metamaterial chip layer 2 arranged up and down, both of which are square grooves with an opening, and their The opening side is relatively fastened to form a closed space, and a layer of porous film 3 is tightly pressed between the cell culture chamber 1 and the metamaterial chip layer 2, and the upper surface of the porous film 3 in the closed space is coated with a Matrigel matrix layer; A resonant ring 6 is arranged at the bottom of the groove of the metamaterial chip layer 2 , and a cell layer 5 is adhered to the upper surface of the resonant ring 6 .

[0051] The material of the cell culture chamber 1 is PDMS (polydimethylsiloxane), and the material of the porous membrane 3 is polycarbonate membrane. The overall size of the porous membrane 3 is consistent with that of...

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Abstract

The invention relates to a terahertz metamaterial chip for cell invasion and migration unmarked detection. A cell culture chamber, a porous film and a metamaterial chip layer are integrated, and by alocally enhanced electric field of the terahertz metamaterial in vertical micron distribution, in-situ unmarked detection of quantity of cells penetrating the porous film is performed to assess movement behaviors such as cell invasion and migration. A whole device is simple in structure, convenient to assemble, small in size, easy to integrate and reusable.

Description

technical field [0001] The invention belongs to the technical field of terahertz measurement, and relates to a terahertz metamaterial chip for label-free detection of cell invasion and migration abilities. Background technique [0002] Invasion and metastasis are the most important biological characteristics of malignant tumors, which are characterized by the malignant cells detaching from the primary site of the tumor, breaking through the basement membrane to infiltrate the surrounding mesenchyme, and entering the blood or lymph fluid to adhere, Proliferate and form tumor cell metastases; this is also an important cause of death in patients with malignant tumors. Accurate evaluation of the invasion and movement ability of tumor cells is very important for the study of tumor metastasis mechanism and the development of anti-tumor drugs. [0003] At present, the experimental methods for assessing cell invasion and motility in vitro mainly include: [0004] 1. Scratch test, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34
CPCC12M23/34C12M33/14C12M41/36
Inventor 赵祥府伟灵张立群王云霞熊瑜
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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