The invention relates to a multi-
enzyme system for separating different tissues-derived
primary culture cells of a normal human and a
mammal. The
system comprises a plurality of 0.0125 to 0.125 weight percent of
trypsin, 0.02 to 0.2 weight percent of
collagenase I-IV, 0.0015 to 0.015 weight percent of
hyaluronidase, 0.02 to 0.2 weight percent of
neutral protease, 0.0015 to 0.015 weight percent of
deoxyribonuclease I, 1 to 10 U / ml
papain, 0.015 to 0.15 weight percent of
pronase, 5 to 50 U / ml
elastase and 0.01 to 0.1 weight percent of separase. The invention also relates to the application of the multi-
enzyme system and a kit formed by the multi-
enzyme system. The multi-
enzyme system of the invention has the characteristics of reasonable design and capacity of separating the different tissues-derived
primary culture cells of the normal human and the
mammal highly specifically and highly sensitively; and the separated
primary culture cells have the characteristics of
high survival rate, high purity, easy
cryopreservation and
recovery, can be used for medicament experimental evaluation, medicament
toxicity test and medicament therapeutic judgment, and are suitable for large-scale popularization and application.