Method for preparing high-quality single-cell suspension by remarkably improving plaque digestion
A digestive juice and plaque technology, applied in the field of plaque digestion, can solve the problems of damage to precision instruments, inability to achieve digestion effect, a large number of cell fragments and dead cells
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[0032] The present invention will be further explained below in conjunction with the experimental group and the accompanying drawings. The following examples are only used to illustrate the present invention, but do not limit the implementation scope of the present invention.
[0033] Example Plaque Tissue Digestion
[0034] 1. Experimental materials
[0035] Enzymes involved in the following experimental groups, control group 1, and control group 2, collagen proteolytic enzyme (Collagenase Ⅰ, sigma, C0130-100MG), collagen proteolytic enzyme Ⅱ (Collagenase Ⅱ, sigma, C6885-100MG), collagen proteolytic enzyme Ⅲ (Collagenase Ⅺ, Solarbio, C8490-100mg), collagenase Ⅳ (CollagenaseⅣ, sigma, C5138-100MG), collagenase Ⅺ (Collagenase Ⅺ, sigma, C7657-100MG), the concentration is 1mg / ml. Trypsin (0.25% Trypsin-EDTA (1×), Gibco-25200072-500ml), hyaluronidase (Hyaluronidase, sigma, H3506-50MG).
[0036] Calcium Chloride (CaCl 2 , sigma, C1016-500g).
[0037] Deoxyribonuclease I (DNase ...
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