Plaque tissue digestion kit and application thereof

Abstract

The invention discloses a plaque tissue digestion kit. The plaque tissue digestion kit comprises L enzyme: Liberase Tm, E enzyme: elastinase, D enzyme: deoxyribonuclease I, and HBSS, wherein the HBSScontains BSA and EDTA. The invention further discloses application of the plaque tissue digestion kit in a plaque tissue. The kit is mainly invented for digesting the plaque tissue; the enzymes are combined, so that the plaque tissue can be quickly digested into single-cell suspension; biological reaction of the plaque tissue is researched on the basis of single cells, so that a condition that a flow cytometry upper machine or follow-up data analysis results are affected by a lot of calcified cell debris, large calcified tissues and the like is avoided.

Description

technical field [0001] The invention relates to the technical field of kits, in particular to a plaque tissue digestion kit and its application. Background technique [0002] The blood of our human body contains a certain amount of lipids, such as triglycerides and cholesterol, etc. Once the content of these lipids increases, it becomes "garbage" in the blood vessels. Among them, low-density protein cholesterol will damage the vascular endothelium after oxidation, resulting in the exposure of the subendothelial tissue, and lipids such as increased triglycerides and low-density protein cholesterol in the blood will enter the blood vessel wall through the damaged endothelium and deposit in blood vessels. Under the endothelium, thicken and harden the endothelium of the blood vessels. At the same time, platelets, which play a hemostatic function in the human body, will quickly adhere and gather in the damaged area, gradually forming atherosclerotic plaques. In the process of s...

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Problems solved by technology

[0003] In the process of plaque processing, if it is not handled well during the mincing process or digestion process, many calcified cell fragments and large pieces of calcified tissue will appear, which will prolong the operation time when filtering the cell suspension and subsequent flow cytometry When the cytometer is testing, the pipeline will be blocked, causing damage to the instrument

Although there are some tissue digestion kits on the market, the tissue processing process is complicated, and due to the particularity of the plaque, the digestion is not complete, and there are a large number of calcified cell fragments and large calcified tissues, which cannot achieve a good digestion effect. Tissue calcification is not well relieved

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