A kind of culture and cryopreservation method of amniotic mesenchymal stem cells
A technology of amniotic mesenchymal stem cells and culture methods, applied in the field of culture and cryopreservation of amniotic mesenchymal stem cells, can solve the problems of low yield, long operation time, complicated process, etc., and achieve high yield and good repeatability , active effect
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Embodiment 1
[0035] This embodiment provides a method for culturing amniotic mesenchymal stem cells, comprising the following steps:
[0036](1) Separation of amniotic membrane tissue: Soak the taken placental tissue in PBS buffer solution, sample blood water as the sample source microbial detection sample, then soak the placental tissue for 2 minutes, and then soak the placental tissue in 75% alcohol for about 1 minute, and use After washing the placental tissue repeatedly with normal saline, the amniotic tissue was gently torn off with tissue forceps, soaked in PBS, and used for the separation of amniotic mesenchymal stem cells, and the normal saline that washed the placenta was finally sampled to detect microorganisms;
[0037] (2) Isolation of amniotic membrane mesenchymal stem cells: cut the amniotic membrane tissue into 1-2mm 2 Add small pieces into a 50mL sterile centrifuge tube, and add an equal volume of mixed enzyme digestion solution (dissolve neutral protease, deoxyribonuclease...
Embodiment 2
[0045] This embodiment provides a method for culturing amniotic mesenchymal stem cells, comprising the following steps:
[0046] (1) Separation of amniotic membrane tissue: Soak the taken placental tissue in PBS buffer, sample blood water as the sample source microbial detection sample, then soak the placental tissue for 3 minutes, then soak the placental tissue with 75% alcohol for about 2 minutes, and use After washing the placental tissue repeatedly with normal saline, the amniotic tissue was gently torn off with tissue forceps, soaked in PBS, and used for the separation of amniotic mesenchymal stem cells, and the normal saline that washed the placenta was finally sampled to detect microorganisms;
[0047] (2) Isolation of amniotic membrane mesenchymal stem cells: cut the amniotic membrane tissue into 1-2mm 2 Add small pieces into a 50mL sterile centrifuge tube, and add 0.8 times the volume of amniotic membrane tissue mixed enzyme digestion solution (dissolve neutral protea...
Embodiment 3
[0055] This embodiment provides a method for culturing amniotic mesenchymal stem cells, comprising the following steps:
[0056] (1) Separation of amniotic membrane tissue: Soak the taken placental tissue in PBS buffer, sample blood water as the sample source microbial detection sample, then soak the placental tissue for 3 minutes, then soak the placental tissue with 75% alcohol for about 2 minutes, and use After washing the placental tissue repeatedly with normal saline, the amniotic tissue was gently torn off with tissue forceps, soaked in PBS, and used for the separation of amniotic mesenchymal stem cells, and the normal saline that washed the placenta was finally sampled to detect microorganisms;
[0057] (2) Isolation of amniotic membrane mesenchymal stem cells: cut the amniotic membrane tissue into 1-2mm 2 Add small pieces into a 50mL sterile centrifuge tube, and add 1.2 times the volume of amniotic membrane tissue mixed enzyme digestion solution (dissolve neutral protea...
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