72 results about "Bacteriological contamination" patented technology

A microfluidic device for culturing cells, termed a microscale cell culture analog (μCCA), is provided. The microfluidic device allows multiple cell or tissue types to be cultured in a physiologically relevant environment, facilitates high-throughput operation and can be used for drug discovery. The microfluidic device uses gravity-induced fluidic flow, eliminating the need for a pump and preventing formation of air bubbles. Reciprocating motion between a pair of connected reservoirs is used to effect the gravity-induced flow in microfluidic channels. Bacterial contamination is reduced and high throughput enabled by eliminating a pump. The microfluidic device integrates a pharmacokinetic-pharmacodynamic (PK-PD) model to enable PK-PD analyses on-chip. This combined in vitro / in silico system enables prediction of drug toxicity in a more realistic manner than conventional in vitro systems.

The invention belongs to the field of edible fungi culture medium processing devices and particularly discloses a stirring device for culture materials of edible fungi. The stirring device comprises a rack, a stirring kettle and a heating tank, wherein the stirring kettle comprises a kettle body, a stirring shaft, a stirring blade and a stirring motor; the kettle body is obliquely fixed on the rack, is cylindrical and comprises a side wall, a sealing ring, an inner wall and an outer wall; a hollow interlayer is arranged between the inner wall and the outer wall which are connected via the sealing ring; a water outlet pipe is arranged on the sealing ring at the relatively low end of the kettle body; steam outlets are uniformly formed in the inner wall; a steam inlet pipe is arranged on the sealing ring at the relatively high end of the kettle body; the stirring blade is spirally wound and fixed on the stirring shaft; the stirring shaft is hinged to the side wall; a belt plate connected with the stirring motor via a belt is arranged on the stirring shaft. The stirring device provided by the invention can not only uniformly stir a culture medium but also effectively protect the culture medium from mould and bacterial contamination.

The invention provides a composite fresh-keeping method of coregonus peled. The composite fresh-keeping method comprises the following technology steps of (1) pretreating coregonus peled; (2) immersing the pretreated coregonus peled in a composite biological fresh-keeping agent; (3) performing vacuum packing; and (4) performing fresh-keeping storage with slurry ice. According to the composite fresh-keeping method, compounding sterilization and oxidation-resistant effects of the composite biological fresh-keeping agent are mainly used, so that growth and propagation of microorganisms in the coregonus peled are effectively restrained, and oxidizing action of fish meat lipid is alleviated; vacuum packing is adopted, so that good effects of protecting color, keeping fresh, guaranteeing quality, preventing bacteriological contamination and the like are achieved; and the fresh-keeping storage with the slurry ice is adopted, so that the fresh-keeping period of the coregonus peled is greatly prolonged, and the composite fresh-keeping method has favorable fresh-keeping effects. According to the method, the fresh-keeping technology and the low-temperature fresh-keeping technology of the biological fresh-keeping agent are performed simultaneously, so that synergistic reaction is completely exerted, and the best effect of fresh keeping can be realized based on that the quality and the freshness of the coregonus peled are guaranteed.

The present invention relates to a continuous method for producing products with molecules or macromolecules attached thereto and apparatus for carrying out this method. The method comprises the steps of: (a) placing the object on or in the proximity of a surface; (b) controlling the electrical potential of the surface with respect to its surroundings; (c) activating the object by exposing it to an electrical discharge; (d) contacting the object with the molecule or macromolecule to be attached. Such macromolecules include bacteriophage. Thus products of methods of the invention are for prevention and amelioration of bacterial contamination of the product of methods of the invention or materials in contact with said products.

The invention provides a toothpick in irreversible color change after use and a method for preparing the toothpick in irreversible color change after use. The method includes the steps: A, processing bamboos or woods to obtain a toothpick body; B, soaking the toothpick body in petal extract with acid indicator functions; C, drying and sterilizing to obtain the toothpick in irreversible color change after use. By soaking of the toothpick in the petal extract, the toothpick has an irreversible color change under the action of intraoral secretion, and consequently cross infection is avoided, and unscrupulous businesses can be prevented from toothpick recovery. The toothpick prepared by the preparation method has an excellent antibacterial effect intrinsically, bacterial contamination of the unused toothpick is avoided, and cross infection is avoided due to irreversible color change of the toothpick.

The present invention relates to compositions and methods for protection against bacterial contamination. The invention provides bactericidal yeast expressing bacteriocin proteins and methods of using the bactericidal yeast.

A treatment system (1) for cleaning biofilm-contaminated components, in particular for cleaning bacterially contaminated surfaces of bone implants or dental implants (30), should enable a particularly compatible and flexible treatment, Wherein the treatment system comprises at least two conductor elements (2, 4) which are connected to a power supply unit (10) to form an electric circuit, one of which can establish electrical contact with the part to be treated. To this end, according to the disclosure, at least one of said conductor elements (2, 4) is implemented as a diamond electrode (26).

The invention relates to a preparation method and antibacterial application of plesiomonas shigelloides phage endolysin. The amino acid sequence of the plesiomonas shigelloides phage endolysin is Seq ID NO.2, and the plesiomonas shigelloides phage endolysin can be prepared into medicine inhibiting bacterial contamination or overgrowth and is applied as bactericide. According to the endolysin, amido bonds between saccharide and peptide of bacterial cell walls or linked bonds between amino acid residues in peptide are hydrolyzed to achieve lysis of host cells finally. The endolysin can act on a host specifically within a short action time and has a wider action spectrum than phage. The endolysin has the advantages of being efficient, specific, free of resistance and the like, and successful application of the endolysin has been reported in the food industry and the pharmaceutical industry at present.

The invention relates to the field of biotechnology renewable energy, in particular to a method for producing endoglucanase by fermenting roughage substrates with a natural symbiotic mixed culture YakQH5, wherein the mixed culture YakQH5 is composed of anaerobic fungi (Neocallimastix frontalis) Composed of Methanobrevibacter gottschalkii, it can degrade different roughages and produce endoglucanase with high enzymatic activity, among which the activity of endoglucanase produced by degrading wheat hulls can reach 1170mU, which has achieved remarkable results Adding compound antibiotics in the fermentation process can also prevent the mixed culture YakQH5 from being polluted by bacteria during the fermentation process, and further improve the efficiency of anaerobic fermentation. The mixed culture adopted in the present invention can be preserved in vitro for survival and passage, which is convenient for popularization and provides convenience for production.

The invention discloses a porous grade composite medical gel scaffold constructed by 3D printing and a preparation method thereof. It is composed of the following raw materials according to the mass percentage: 7-30% of hydroxyapatite, and 0.7% of drug-loaded mesoporous silica ‑2%, gluconolactone 11‑16%, and the balance is sodium alginate. The present invention combines hydroxyapatite with alginate solution, and prepares alginate / hydroxyapatite hydrogel material through a simple composite gel approach, which can obtain good biological activity, compatibility, and drug loading and mitigation interpreted medical materials. The 3D printed gel solution in the present invention is added with drug-loaded mesoporous silica, which can play a thickening role and adjust the viscosity of the formula, so that the pre-crosslinked hydrogel can be printed under 3D printing conditions The gel stent is well formed; in addition, the drug load can realize the slow release of the drug, which can prevent the 3D stent from being contaminated by bacteria and play an antibacterial and sterilizing role.

The invention relates to a method for preparing composite hydrophobic nano powder-coated liquid marbles, which belongs to the technical field of coating materials. The invention uses nano-zinc oxide powder, nano-titanium dioxide powder and nano-alumina powder as raw materials to prepare a composite hydrophobic nano-powder that wraps liquid, and can effectively reduce the liquid in the marble by wrapping the hydrophobic nano-zinc oxide powder. The risk of harmful bacterial contamination, thereby improving the safety of liquid marbles, nano-titanium dioxide has a strong UV shielding effect, and is effective against Bacillus subtilis var. niger, Pseudomonas aeruginosa, E. The extinction rate reaches 98~99%, which can effectively improve the medical use and safety of liquid marbles. Nano-alumina is in the state of white fluffy powder, which has the advantages of large specific surface area, high porosity, strong adsorption and good formability. It can effectively adsorb on the surface of the liquid, and effectively adsorb and bond other particles on the surface of the liquid, thus forming a stable liquid marble.

The invention discloses a method for evaluating the pathogenicity of grape canker bacteria on grapes. The method is: take the bacterial strains cultured on the PDA plate, take the bacteria cake and place it in the improved Fries liquid medium for static culture for 14 days, centrifuge to collect the supernatant, filter with a pinhole filter to remove mycelia, spores and bacterial contamination , that is, the preparation of the crude toxin stock solution is completed. Select new young leaves of annual green branches, after surface disinfection, take out a leaf disc with a diameter of 10mm, absorb 10 μL of crude toxin stock solution and inoculate it in the central part of the leaf disc, measure the lesion area after 3 days, and evaluate the potency of grape canker sore crude toxin stock solution pathogenicity. The invention has the advantages of simple operation, low cost and good repeatability, and provides technical support for the research on the effect of the mycotoxin in the pathogenic process of grape canker bacterium and the research on the selection of grape varieties resistant to grape canker.

The invention discloses a mask outer packaging bag capable of invalidating regularly and a production method thereof, and relates to the field of packaging bags, a biodegradable film with good biodegradability is prepared firstly, then the biodegradable film is coated with an antibacterial and hydrophobic coating with good antibacterial and bacteriostatic performance and water-repellent and stain-resistant performance, and a timed invalidating film is obtained; the mask outer packaging bag capable of invalidating regularly is obtained by cutting the timed invalidating film into a packaging bag, the mask outer packaging bag capable of invalidating regularly has good antibacterial and hydrophobic performance, the antibacterial performance can ensure that the safety is good and bacterial pollution is avoided when the mask is loaded, and the hydrophobic performance can ensure that the packaging bag is not easy to stick dust and rot when meeting water, and the sanitation degree of the mask is further ensured; after the mask outer packaging bag capable of invalidating regularly is discarded, the biodegradable film is exposed after the mask outer packaging bag is crushed, and the mask outer packaging bag can be quickly degraded through good biodegradability.

Disclosed herein are catheter assemblies (100) with a locator tip (134) proximal to the distal tip (112) of a catheter tube (110). The locator tip is sized to remain outside a female urethra and configured to allow the catheter tube to pass there through. As an example, the locator tip allows a woman using a urinary catheter to target her urethra without exposing the catheter tube to tissue untilthe urethra is located and insertion is initiated. This avoids infecting the urethra with a catheter tube that has been contaminated by bacteria around the urethra.

The invention discloses a gas humidification liquid and a preparation method thereof. The gas humidification liquid is an aqueous solution containing at least ε-polylysine and sodium dehydroacetate as active ingredients, wherein the concentration of ε-polylysine is 3-60 μg / mL, and the concentration of sodium dehydroacetate is 50 μg / mL. ~440 μg / mL. The preparation method of the gas humidification liquid is as follows: First, dissolve other active ingredients except ε-polylysine in purified water at room temperature, heat up to 45-85°C, add ε-polylysine and stir to dissolve, and then Add purified water to the desired concentration, and sterilize to obtain a humidification solution. The humidifying solution of the present invention has good storage stability and is not easy to produce precipitates; the humidifying solution of the present invention has excellent antibacterial broad spectrum and excellent effect, effectively ensuring that the humidifying solution is not contaminated by bacteria during clinical use; in addition, the preparation method It is simple and fast, the active ingredients are easier to dissolve, and has good clinical value and social significance of popularization and use.